Is Cell‐to‐Cell Contact Necessary for the Expression of the N‐Gene in <i>Nicotiana tabacum</i> cv. Xanthi nc. Plants Infected by TMV?

Gulyás, Anna; Farkas, Gabriella

doi:10.1111/j.1439-0434.1978.tb04211.x

Cited by 22 publications

(3 citation statements)

References 4 publications

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“…Thus, the process of virus movement may trigger hypersensitivity, which implicates intercellular signaling in this type of HR. Support for this possibility comes from experiments in which cell‐to‐cell contacts were disrupted in tobacco carrying the N gene, resulting in the prevention of necrotic lesion formation in infected leaves (Gulyas and Farkas, 1978). Likewise, TMV will not induce hypersensitive cell death in N genotype tobacco protoplasts whose plasmodesmata are not intact (Otsuki et al ., 1972), while the HR does occur in N genotype tobacco callus cultures whose plasmodesmata are intact (Beachy and Murakishi, 1971).…”

Section: Introductionmentioning

confidence: 99%

Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor

Cooper¹

2001

The Plant Journal

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SummaryHypersensitive reactions to plant diseases are typically mediated by R genes. Many R genes that have been cloned only confer resistance to a particular pathogen. However, Chenopodium spp. have multivirus hypersensitive resistance, thus making the understanding of this broad-spectrum resistance mechanism attractive. Using tobacco mosaic virus (TMV) tagged with green¯uorescent protein to follow infection over time, cDNA-AFLP to ®nd genes up-regulated during virus infection in C. amaranticolor and quantitative RT±PCR to accurately measure gene expression at different time points, the ®rst dissection of this signi®cant defense response pathway is presented. The detected diseaseexpressed sequences in C. amaranticolor (DESCA) are similar to those that encode p450 monooxegenases, hypersensitivity-related genes, cellulases, ABC transporters, receptor-like kinases, serine/threonine kinases, phosphoribosylanthranilate transferases and hypothetical R genes, many of which are associated with pathogen defense in other plants. The expressions of these DESCA genes are also induced by infection with the taxonomically distinct tobacco rattle virus (TRV) in C. amaranticolor. In particular, DESCA1, one of the gene fragments from C. amaranticolor that lacks similarity to any other sequence in the GenBank database, is induced at least 200 fold 4 d after infection (dai) by both TMV and TRV. The potential role of DESCA genes in a C. amaranticolor multivirus defense response with regard to their levels and time of gene expression is discussed.

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Section: Introductionmentioning

confidence: 99%

Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor

Cooper¹

2001

The Plant Journal

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“…Par ailleurs Tabsorption du precurseur par les cellules varie avec la pression osmotique du milieu d'incubation (RUESINK 1978). De fortes pressions diminuent Li vitesse d'absorption et en meme temps empechent le deroulement normal de la reaction d'hypersensibilite , GULYAS et FARKAS 1978, GOUTTS 1978, KOPP et al 1979. G*est pourquoi nous avons essaye une methode de flottation de tissus sur milieu isotonique qui, a notre avis, devait permettre de surmonter ces difficultes.…”

Section: Resultats Et Discussion 1 Mise Au Point D'une Methode De Munclassified

Multiplication du Virus de la Mosaïque du Tabac dans des Hôtes à Réponse Systémique ou Nécrotique: Approche Biochimique à l'Étude de la Résistance Hypersensible aux Virus

Konaté

Kopp

Fritig

1982

Journal of Phytopathology

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“…The reason for this HR inactivation at high temperatures is not known. Previous studies suggested that high temperatures inhibit an early event in the initiation of HR induced by TMV ( Gulyas & Farkas 1978; Samuel 1931; Takahashi 1975). More recent studies have suggested that the temperature sensitivity of the N ‐mediated response is manifested at the level of interaction between the virus and the defence response mechanism ( Padgett et al .…”

Section: Discussionmentioning

confidence: 97%

The helicase domain of the TMV replicase proteins induces the N‐mediated defence response in tobacco

et al. 1999

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Tobacco mosaic virus (TMV) induces the hypersensitive response (HR) in tobacco plants containing the N gene. This defence response is characterized by cell death at the site of virus infection and inhibition of viral replication and movement. A previous study indicated that a portion of the TMV replicase containing a putative helicase domain is involved in HR induction. Here, this observation is confirmed and extended by showing that non-viral expression of a 50 kDa TMV helicase fragment (p50) is sufficient to induce the N-mediated HR in tobacco. Like the HR elicited by TMV infection, transgenic expression of p50 induces a temperature-sensitive defence response. We demonstrate that recombinant p50 protein has ATPase activity, as suggested by the presence of conserved sequence motifs found in ATPase/helicase enzymes. A point mutation that alters one of these motifs abolishes ATPase activity in vitro but does not affect HR induction. These results suggest that features of the TMV helicase domain, independent of its enzymatic activity, are recognized by N-containing tobacco to induce TMV resistance.

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Is Cell‐to‐Cell Contact Necessary for the Expression of the N‐Gene in Nicotiana tabacum cv. Xanthi nc. Plants Infected by TMV?

Cited by 22 publications

References 4 publications

Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor

Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor

Multiplication du Virus de la Mosaïque du Tabac dans des Hôtes à Réponse Systémique ou Nécrotique: Approche Biochimique à l'Étude de la Résistance Hypersensible aux Virus

The helicase domain of the TMV replicase proteins induces the N‐mediated defence response in tobacco

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