Is "Campylobacter upsaliensis" an unrecognised cause of human diarrhoea?

Goossens, Herman; Vlaes, L; Boeck, M. De; Levy, Jack S.; Mol, Patrick De; Butzler, Jean‐Paul; Kersters, Karel; Pot, Bruno; Vandamme, Peter

doi:10.1016/0140-6736(90)90359-d

Cited by 93 publications

(45 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others have found that a filtration technique was important for sufficient isolation of C. upsaliensis (15,23). Goossens (13) found a pronounced difference between selective media and the filtration technique in favor of the filtration, but the two selective media that were used contained 30 and 32 mg of cefoperazone/liter, which probably could have been inhibitory to the growth of C. upsaliensis.…”

Section: Discussionmentioning

confidence: 99%

“…In early studies, only C. jejuni and C. coli were reported. However, in 1983, a new species named C. upsaliensis was isolated for the first time from canine feces (37) and further characterized and described in both human feces in 1990 (15) and dog feces in 1991 (36). Since then, C. upsaliensis has been increasingly prevalent in dogs, according to published reports, and even more prevalent than C. jejuni, comprising 64 to 82% (4,27,35,37) of the strains isolated from dogs.…”

mentioning

confidence: 99%

“…In surveys, Campylobacter spp. have been isolated from between 2.8 and 10.5% of gastroenteritis cases in Utah (17), Australia (19), Belgium (15), Sweden (24), and Denmark (10) and from 21.8% of children in South Africa (20). The majority of campylobacteriosis cases in the reported surveys are caused by Campylobacter jejuni or Campylobacter coli, whereas Campylobacter upsaliensis accounts for less than 1% of the total number of cases, except for the South African survey, in which C. upsaliensis was isolated from 4.9% of 20,458 children with gastroenteritis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark

et al. 2004

View full text Add to dashboard Cite

The Campylobacter excretion patterns of 26 domestic pet dogs were described in a longitudinal study. The dogs entered the study between 3 and 8 months of age and were monitored until 2 years of age. They were tested monthly for Campylobacter carriage in stool samples that were cultured on the Campylobacter-selective media CAT and modified CCDA agar at 37 and 42°C. This study comprised 366 fecal swab samples, of which 278 (76.2%) were found to be Campylobacter positive, with the following distribution of species: 75.0% Campylobacter upsaliensis, 19.4% Campylobacter jejuni, 2.1% Campylobacter lari, 0.7% Campylobacter coli, and 2.8% Campylobacter spp. Isolates were typed by pulsed-field gel electrophoresis (PFGE) to elucidate the strain excretion pattern. All study dogs excreted Campylobacter spp. during the study period. At 3 months of age, 60% of the dogs carried Campylobacter, increasing to nearly 100% carriers at 1 year of age, whereafter the carriage rate decreased to 67% at 24 months of age. The PFGE types showed that individual dogs were often colonized by unique strains of C. upsaliensis for several months, up to 21 months or longer. These C. upsaliensis strains were either clonal (or underwent concurrent minor mutative changes) or independent strains. In contrast, the excreted C. jejuni isolates were much more diverse and, in most cases, only seen in one sample from each dog. A high degree of diversity among different dogs was seen. We conclude that young domestic pet dogs excreted Campylobacter spp. during the majority of their puppyhood and adolescent period. In general C. upsaliensis strains were excreted for months, with short-term interruptions by or cocolonization with other transitory Campylobacter spp., predominantly C. jejuni. C. jejuni was more prevalent in dogs between 3 months and 1 year of age than in dogs between 1 and 2 years of age.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Some of these involve the direct microscopic detection of the microorganism in stool, the recovery of the organism from culture following the use of a filtration method, or the use of a specialized selective medium for the enhanced recovery of Campylobacter from stool (9). Most clinical laboratories do not use the direct microscopic or filtration method, because microscopy is insensitive (22,25,33), and filtration is cumbersome and may lack sensitivity (11). The use of a selective medium is recommended for the optimal recovery of Campylobacter from stool samples (9).…”

mentioning

confidence: 99%

Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of Campylobacter Enteric Infections

et al. 2010

View full text Add to dashboard Cite

Campylobacter enteritis is a food-borne or waterborne illness caused almost exclusively by Campylobacter jejuni and, to a lesser extent, by Campylobacter coli. These organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the United States and the leading cause of enteric infection throughout the world. The conventional approach to the laboratory diagnosis of Campylobacter enteritis is based on the recovery of the organism from a stool specimen, which requires the use of a specialized medium incubated at 42°C for several days in an artificially created microaerophilic environment. Recently, several commercially available enzyme immunoassays (EIAs) have been developed for the direct detection of C. jejuni and C. coli in stool specimens. This study compared conventional culture with three EIA methods, the Premier CAMPY EIA (Meridian Bioscience, Cincinnati, OH), the ProSpecT Campylobacter EIA (Remel, Lenexa, KS), and the ImmunoCard STAT! CAMPY test (Meridian Bioscience, Cincinnati, OH), for the detection of C. jejuni and C. coli in 485 patient stool samples. Discordant results were arbitrated by using an in-house, real-time PCR assay that was developed and validated by a public health reference laboratory. Following analyses of the discrepant specimens by PCR, the sensitivity and specificity of both the Premier CAMPY and ProSpecT Campylobacter EIAs were 99.3% and 98%, respectively, while the ImmunoCard STAT! CAMPY test had a sensitivity of 98.5% and a specificity of 98.2%. By use of the PCR test as the reference standard, culture detected 127 of 135 Campylobacter-positive stool specimens, yielding a sensitivity of 94.1%. These results showed that the three EIAs evaluated in this study provide a rapid and reliable alternative for the laboratory diagnosis of enteric infections with C. jejuni and C. coli and that conventional culture may no longer be recognized as the "gold standard" for diagnosis.

show abstract

“…C. laridis is a recognized bird-associated species [8] and has previously been isolated from a resident with enteritis in the affected area (S. J. Hudson, personal communication). It is common practice in routine diagnostic laboratories to use only one selective media which may be inadequate to isolate some Camrpylobacter species [9]. Further the identification of campylobacters is often rudimentary and the hippurate test may give doubtful results [10].…”

Section: Discussionmentioning

confidence: 99%

Jackdaws and magpies as vectors of milkborne human campylobacter infection

et al. 1991

View full text Add to dashboard Cite

SUMMARYIn 1990 we reported that milk bottles pecked by jackdaws and magpies were a probable source of human campylobacter infection. During April to June 1990 an extended study of campylobacter infections was carried out in the Gateshead area. Prior to the study a health education programme was undertaken in an attempt to reduce human infection. Fifty-nine cases of human infection were recorded and 52 were interviewed. Thirty were entered into a case control study which demonstrated a very strong association between consumption of pecked milk and human campylobacter infection (X2 = 12 6, P < 00004). It was estimated that between 500 and 1000 jackdaws (Corvus monedula) were present in the area where milk bottles were pecked and 63 isolates of campylobacter were made from the bill and cloaca. Target bottles were put out in the early mornings and campylobacters were isolated from 12 of 123 pecked bottles. Typing of the campylobacters revealed a wide distribution of strains amongst birds, pecked milk and human infections. The health education programme had only limited success.

show abstract

Is "Campylobacter upsaliensis" an unrecognised cause of human diarrhoea?

Cited by 93 publications

References 16 publications

Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark

Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark

Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of Campylobacter Enteric Infections

Jackdaws and magpies as vectors of milkborne human campylobacter infection

Contact Info

Product

Resources

About