Is bioengineering a possibility in gastrointestinal disorders?

Bitar, Khalil N.; Zakhem, Elie

doi:10.1586/17474124.2015.1103178

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…The cells must be expanded in vitro while maintaining their in vivo properties, which is complex with individually isolated/PSC-generated cell types. In vivo , the cells require interaction with other cell populations to maintain and regulate their phenotype and function ( Bitar and Zakhem, 2015 ). Isolated smooth muscle cells (ISMCs) from rat intestines have shown to develop an altered immature phenotype that favors proliferation over differentiation when cultured in vitro .…”

Section: Gastrointestinal Tractmentioning

confidence: 99%

Regenerative medicine technologies applied to transplant medicine. An update

Petrosyan

Montali²,

Peloso³

et al. 2022

Front. Bioeng. Biotechnol.

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Regenerative medicine (RM) is changing how we think and practice transplant medicine. In regenerative medicine, the aim is to develop and employ methods to regenerate, restore or replace damaged/diseased tissues or organs. Regenerative medicine investigates using tools such as novel technologies or techniques, extracellular vesicles, cell-based therapies, and tissue-engineered constructs to design effective patient-specific treatments. This review illustrates current advancements in regenerative medicine that may pertain to transplant medicine. We highlight progress made and various tools designed and employed specifically for each tissue or organ, such as the kidney, heart, liver, lung, vasculature, gastrointestinal tract, and pancreas. By combing both fields of transplant and regenerative medicine, we can harbor a successful collaboration that would be beneficial and efficacious for the repair and design of de novo engineered whole organs for transplantations.

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Section: Gastrointestinal Tractmentioning

confidence: 99%

Regenerative medicine technologies applied to transplant medicine. An update

Petrosyan

Montali²,

Peloso³

et al. 2022

Front. Bioeng. Biotechnol.

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“…Tissue engineering provides an alternative approach that has been shown to be promising in the field of gut bioengineering (Bitar and Zakhem, ). This group has previously developed a technique to engineer different gut smooth muscle layers with the appropriate alignment (Hecker et al, ; Raghavan et al, ).…”

Section: Introductionmentioning

confidence: 99%

Biomechanical properties of an implanted engineered tubular gut-sphincter complex

Zakhem

Bahrawy

Orlando

et al. 2016

J Tissue Eng Regen Med

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Neuromuscular diseases of the gut alter the normal motility patterns. Although surgical intervention remains the standard treatment, preservation of the sphincter attached to the rest of the gut is challenging. The present study aimed to evaluate a bioengineered gut-sphincter complex following its subcutaneous implantation for 4 weeks in rats. Engineered innervated human smooth muscle sheets and innervated human sphincters with a predefined alignment were placed around tubular scaffolds to create a gut-sphincter complex. The engineered complex was subcutaneously implanted in the abdomen of the rats for 4 weeks. The implanted tissues were vascularized. In vivo manometry revealed luminal pressure at the gut and the sphincter zone. Tensile strength, elongation at break and Young's modulus of the engineered complexes were similar to those of native rat intestine. Histological and immunofluorescence assays showed maintenance of smooth muscle circular alignment in the engineered tissue, maintenance of smooth muscle contractile phenotype and innervation of the smooth muscle. Electrical field stimulation induced relaxation of the smooth muscle of both the sphincter and the gut parts. Relaxation was partly inhibited by nitric oxide inhibitor indicating nitrergic contribution to relaxation. The present study has demonstrated for the first time a successfully developed and subcutaneously implanted a tubular human-derived gut-sphincter complex. The sphincteric part of Tubular Gut-Sphincter Complex (TGSC) maintained the basal tone characteristic of a native sphincter. The gut part also maintained its specific neuromuscular characteristics. The results of this study provide a promising therapeutic approach to restore gut continuity and motility. Copyright © 2016 John Wiley & Sons, Ltd.

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Bioengineering the gut: future prospects of regenerative medicine

Bitar

Zakhem

2016

Nat Rev Gastroenterol Hepatol

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Functions of the gastrointestinal tract include motility, digestion and absorption of nutrients. These functions are mediated by several specialized cell types including smooth muscle cells, neurons, interstitial cells and epithelial cells. In gastrointestinal diseases, some of the cells become degenerated or fail to accomplish their normal functions. Surgical resection of the diseased segments of the gastrointestinal tract is considered the gold-standard treatment in many cases, but patients might have surgical complications and quality of life can remain low. Tissue engineering and regenerative medicine aim to restore, repair, or regenerate the function of the tissues. Gastrointestinal tissue engineering is a challenging process given the specific phenotype and alignment of each cell type that colonizes the tract - these properties are critical for proper functionality. In this Review, we summarize advances in the field of gastrointestinal tissue engineering and regenerative medicine. Although the findings are promising, additional studies and optimizations are needed for translational purposes.

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Is bioengineering a possibility in gastrointestinal disorders?

Cited by 3 publications

References 20 publications

Regenerative medicine technologies applied to transplant medicine. An update

Regenerative medicine technologies applied to transplant medicine. An update

Biomechanical properties of an implanted engineered tubular gut-sphincter complex

Bioengineering the gut: future prospects of regenerative medicine

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