Is bioaugmentation a feasible strategy for pollutant removal and site remediation?

Fantroussi, Saïd El; Agathos, Spiros N.

doi:10.1016/j.mib.2005.04.011

Cited by 586 publications

(287 citation statements)

References 58 publications

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“…It is commonly known that most of the laboratory constructed (or studied) strains are not able to survive under natural, environmental conditions. In many field experiments, a rapid decline in the inoculum size or massive cell death have frequently been observed (El Fantroussi and Agathos 2005), and one of the best ways to avoid such problems is the application of the plasmid-mediated bioaugmenation approach.…”

Section: Discussionmentioning

confidence: 99%

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Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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Plasmid pSinA of Sinorhizobium sp. M14 (Alphaproteobacteria) is the first described, natural, self-transferable plasmid harboring a complete set of genes for oxidation of arsenite. Removal of this plasmid from cells of the host strain caused the loss of resistance to arsenic and heavy metals (Cd, Co, Zn and Hg) and abolished the ability to grow on minimal salt medium supplemented with sodium arsenite as the sole energy source. Plasmid pSinA was introduced into other representatives of Alphaproteobacteria which resulted in acquisition of new abilities concerning arsenic resistance and oxidation, as well as heavy metals resistance. Microcosm experiments revealed that plasmid pSinA can also be transferred via conjugation into other indigenous bacteria from microbial community of As-contaminated soils, including representatives of Alpha-and Gammaproteobacteria. Analysis of "natural" transconjugants showed that pSinA is functional (expresses arsenite oxidase) and is stably maintained in their cells after approximately 60 generations of growth under nonselective conditions. This work clearly demonstrates that pSinA is a self-transferable, broad-host-range plasmid, which plays an important role in horizontal transfer of arsenic metabolism genes.

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Section: Discussionmentioning

confidence: 99%

“…In many bioaugmentation field experiments, a rapid decline in the inoculum size or massive cell death have frequently been observed (El Fantroussi and Agathos 2005). The use of bacterial strains that contain conjugal or mobilizable plasmids carrying the desired genes (e.g.…”

Section: Functionality Of the Psina Tra Modulementioning

confidence: 99%

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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“…In order to reach 88% of the removal efficiency, as shown in Table 2, seven days were required for the strain P. aeruginosa G23. Apparently, our strain had the lower growth rate, which is due to incomplete adaptation (El Fantroussi et al, 2005). It is known that in order to reach more efficient removal of pollutant bacterium must be pre-adapted in the medium containing the contaminant, which makes the bacteria more competitive.…”

Section: Biodegradation In Model Solutions With Animal Fatsmentioning

confidence: 88%

“…First, lipases play a key role in the metabolism of strains that destroy FOGs. Second, a poor performance of indigenous microorganisms in FOGs polluted environment could be effectively intensified by using bioaugmentation strategy (El Fantroussi et al, 2005) which needs additions of new effective strains. Third, some inoculated microorganisms in modern biotechnological preparations produced for biological treatment of FOGs wastes, are not resistant in open systems and require periodic additions of inocula to achieve a high performance treatment (Loperena et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Screening of lipid degrading microorganisms for wastewater treatment

Sarmurzina¹,

Kozhakhmetov²,

Anuarbekova³

et al. 2013

MJM

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Aims: Fats, oils and greases (FOG) are poorly removable materials in wastewater treatment systems. The aim of this work is to find the most suitable strain(s) for a biological treatment technology of FOGs polluted wastewaters. Methodology and results:The 142 microorganisms from polluted environment were screened for lipase activity (LA) by sequentially using assays on agar-Tween 80, agar-fats, and turbidimetrically measuring the quantity of calcium salts with fatty acids. The isolates G23, G30, and Zb32 showed highest units of LA and were identified by sequence analysis of 16S rRNA genes. Lipid masses were determined gravimetrically after chloroform/ethyl alcohol extraction. In the model solutions with animal fats the strain Pseudomonas aeruginosa G23 reduced mass fractions of mutton fat, beef tallow, and lard by 79±5%, 88±4%, and 80±6% respectively. Under the same conditions Aeromonas punctata G30 reduced: 65±3%, 60±8%, and 75±4 %, and P. aeruginosa Zb32 reduced: 47±5 %, 52±6% and 73±7%. In the model solutions with FOGs trap specimens as a carbon source from the local cafeteria the strains P. aeruginosa G23, A. punctata G30, and P. aeruginosa Zb32 reduced a lipid mass fraction by 61.5±7%, 45.2±5%, and 37.5±3% respectively. Conclusion, significance and impact of study: The strain P. aeruginosa G23 is the most effective lipid-degrading microorganism and the best candidate to use in biological treatment technology of FOGs polluted wastewater in Kazakhstan.

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“…Successful performance of the PRBBs requires a continuous maintenance of the microorganisms capable of utilizing the target contaminants, which in turn requires a continuous supply of the necessary nutrients and the target contaminants (Fantroussi and Agathos, 2005). However, in groundwater, the contaminants are often present as discontinuous plumes.…”

Section: Gel Electrophoresismentioning

confidence: 99%

In situ Groundwater Remediation Using Enricher Reactor – Permeable Reactive Biobarrier

Kasi¹,

McEvoy²,

Padmanabhan³

et al. 2010

proc water environ fed

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Permeable reactive biobarrier (PRBB) is a flow-through zone where microorganisms degrade contaminants in groundwater. Discontinuous presence of contaminants in groundwater causes performance loss of a PRBB in removing the target contaminant. A novel enricher reactor (ER) -PRBB system was developed to treat groundwater with contaminants that reappear after an absence period. ER is an offline reactor for enriching contaminant degraders, which were used for augmenting PRBB to maintain its performance after a period of contaminant absence. The ER-PRBB concept was initially applied to remove benzene that reappeared after absence periods of 10 and 25 days. PRBBs without ER augmentation experienced performance losses of up to 15% higher than ER-PRBBs.The role of inducer compounds in the ER to enrich bacteria that can degrade a mixture of benzene, toluene, ethylbenzene, and xylene (BTEX) was investigated with an objective to minimize the use of toxic chemicals as inducers. Three inducer types were studied: individual BTEX compounds, BTEX mixture, and benzoate (a non toxic and a common intermediate for BTEX biodegradation). Complete BTEX removal was observed for degraders enriched on all three inducer types; however, the removal rates were dependent on the inducer type. Degraders enriched on toluene and BTEX had the highest degradation rates for BTEX of 0.006 to 0.014 day -1 and 0.006 to 0.012 day -1 , respectively, while degraders enriched on benzoate showed the lowest degradation rates of 0.004 to 0.009 day -1 .The ER-PRBB technique was finally applied to address the performance loss of a PRBB due to inhibition interactions among BTEX, when the mixture reappeared after a iv 10 day absence period. The ER-PRBBs experienced minimal to no performance loss, while PRBBs without ER augmentation experienced performance losses between 11% and 35%. Presence of ethanol during the BTEX absence period increased the performance loss of PRBB for benzene removal. PRBBs augmented with degraders enriched on toluene alone overcame the inhibition interaction between benzene and toluene indicating that toluene can be used as a single effective inducer in an ER. The ER-PRBB was demonstrated to be a promising remediation technique and has potential for applications to a wide range of organic contaminants.v ACKNOWLEDGEMENTS

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Is bioaugmentation a feasible strategy for pollutant removal and site remediation?

Cited by 586 publications

References 58 publications

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Screening of lipid degrading microorganisms for wastewater treatment

In situ Groundwater Remediation Using Enricher Reactor – Permeable Reactive Biobarrier

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