IS 1236, a newly discovered member of the IS3 family, exhibits varied patterns of insertion into the Acinetobacter calcoaceticus chromosome

Gerischer, Ulrike; Da, D'Argenio; Ln, Ornston

doi:10.1099/13500872-142-7-1825

Cited by 35 publications

(48 citation statements)

References 15 publications

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“…IS1236 was first discovered in studies of mutations that prevent the degradation of p-hydroxybenzoate (18,19). The spontaneous insertion of IS1236 plays a significant role in the inactivation of genes for aromatic compound catabolism in two different chromosomal regions of ADP1 (18,19,26,41).…”

Section: Resultsmentioning

confidence: 99%

“…In A. baylyi ADP1, there is a single type of IS element, IS1236 (5,18,19,25). In the chromosome, there are six copies of IS1236, five of which are identical.…”

mentioning

confidence: 99%

“…2), two adjacent copies (IS1236_2 and IS1236_3) can function as a composite transposon (41). IS1236 belongs to the large IS3 family, members of which transpose using a replicative excision and conservative integration mechanism, also known as the copy-andpaste method of transposition (15,18). As a result, the element is retained in its original locus as well as being inserted at a new location (8,15,30,37,38).…”

mentioning

confidence: 99%

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Analysis of IS 1236 -Mediated Gene Amplification Events in Acinetobacter baylyi ADP1

et al. 2012

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Recombination between insertion sequence copies can cause genetic deletion, inversion, or duplication. However, it is difficult to assess the fraction of all genomic rearrangements that involve insertion sequences. In previous gene duplication and amplification studies of Acinetobacter baylyi ADP1, an insertion sequence was evident in approximately 2% of the characterized duplication sites. Gene amplification occurs frequently in all organisms and has a significant impact on evolution, adaptation, drug resistance, cancer, and various disorders. To understand the molecular details of this important process, a previously developed system was used to analyze gene amplification in selected mutants. The current study focused on amplification events in two chromosomal regions that are near one of six copies of the only transposable element in ADP1, IS 1236 (an IS 3 family member). Twenty-one independent mutants were analyzed, and in contrast to previous studies of a different chromosomal region, IS 1236 was involved in 86% of these events. IS 1236 -mediated amplification could occur through homologous recombination between insertion sequences on both sides of a duplicated region. However, this mechanism presupposes that transposition generates an appropriately positioned additional copy of IS 1236 . To evaluate this possibility, PCR and Southern hybridization were used to determine the chromosomal configurations of amplification mutants involving IS 1236 . Surprisingly, the genomic patterns were inconsistent with the hypothesis that intramolecular homologous recombination occurred between insertion sequences following an initial transposition event. These results raise a novel possibility that the gene amplification events near the IS 1236 elements arise from illegitimate recombination involving transposase-mediated DNA cleavage.

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Section: Resultsmentioning

confidence: 99%

“…In A. baylyi ADP1, there is a single type of IS element, IS1236 (5,18,19,25). In the chromosome, there are six copies of IS1236, five of which are identical.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of IS 1236 -Mediated Gene Amplification Events in Acinetobacter baylyi ADP1

et al. 2012

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“…As example; the same expanded-spectrum beta-lactamase PER-1 gene was found in a chromosomally-integrated transposon in some A. baumannii isolates, and on a conjugative plasmid in others (Poirel et al, 2005); transposon Tn5613 of unknown function (but closely linked to various aromatic catabolic genes) is integrated into the chromosome of Acinetobacter sp. ADP1 (section 6.1.1) (Gerischer et al, 1996;Barbe et al, 2004); and conjugative transposon Tn2009 of A. junii 329 is located on the chromosome, is indistinguishable from a Streptococcus pneumoniae element (indicationg recent horizontal exchange), and can be transferred to various other Gram negative bacteria (Ojo et al, 2006).…”

Section: Transposons and Integronsmentioning

confidence: 99%

“…The ADP1 genome has a small fraction of repetitive sequences (1.6%) ranging from a few short repeats to several complex ones. The longest repeated sequence is known as transposon Tn5613 (Gerischer et al, 1996;Barbe et al, 2004). Genome analysis has also revealed two principal prophage regions.…”

mentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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