IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth

Vert, Grégory; Grotz, Natasha; Dédaldéchamp, Fabienne; Gaymard, Frédéric; Guerinot, Mary Lou; Briat, Jean François; Curie, Catherine

doi:10.1105/tpc.001388

Cited by 1,426 publications

(1,431 citation statements)

References 42 publications

Supporting

Mentioning

1,337

Contrasting

Unclassified

Order By: Relevance

“…In summary, the GUS activities driven by the subgroup Ib BHLH promoters overlapped with GUS activity patterns observed for pFIT and pIRT1 (see also Vert et al 2002;Jakoby et al 2004). pFIT-GUS staining seemed to occur closer to the tip relative to staining patterns of the four BHLH promoters tested.…”

Section: Analysis Of Subgroup Ib Bhlh Promoter-driven Gus Activitysupporting

confidence: 57%

“…0 Fe means 0 M Fe plus 50 M ferrozine. Mean values § SD were determined for three to eight measurements tip and in the upper root zones, induced by low iron and down-regulated in Wt loss of function mutants (Eide et al 1996;Robinson et al 1999;Vert et al 2002;Connolly et al 2003;Colangelo and Guerinot 2004;Jakoby et al 2004). FIT was also mainly expressed in roots near the root tip and in the upper root zones, induced by low iron and its promoter was down regulated in Wt loss of function mutants (Colangelo and Guerinot 2004;Jakoby et al 2004;this work).…”

Section: Discussionmentioning

confidence: 99%

“…To test whether other iron mobilization mutants with chlorotic phenotypes were capable of expressing and upregulating subgroup Ib BHLH genes, we compared gene expression in frd1-1 mutant plants (FRO2 mutant, devoid of iron reductase activity; Robinson et al 1999), irt1-1 mutant plants (devoid of iron transporter activity; Vert et al 2002), and man1 mutant plants (also known as frd3-3, improper iron transport to the shoot; Rogers et al 2002). Generally, these mutant plants were more chlorotic than wild type plants upon iron deWciency.…”

Section: Bhlh038 Bhlh039 Bhlh100 and Bhlh101 Gene Expression In Iromentioning

confidence: 99%

“…This iron mobilization system is strictly regulated. At low iron supply FRO2 and IRT1 genes are induced whereas upon suYcient iron supply the genes are down regulated (Eide et al 1996;Robinson et al 1999;Vert et al 2002). A central transcriptional regulator in this process in tomato is the basic helix-loop-helix (bHLH) factor FER; the A. thaliana ortholog is encoded by the gene FIT (= fer-like iron deWciency-induced transcription factor, previously known as FRU and FIT1, gene number At2 g28160; Bauer et al 2004;Colangelo and Guerinot 2004;Jakoby et al 2004;Yuan et al 2005; FIT was renamed by Bauer, Guerinot and Ling).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Hong-yu

Klatte

Jakoby

et al. 2007

Planta

224

202

View full text Add to dashboard Cite

Networks of transcription factors control physiological, developmental and environmental responses. Root iron acquisition responses are controlled by the essential bHLH protein FIT. Recently, two group Ib BHLH genes were reported to be iron deWciency-regulated. Here, we studied expression patterns of these two group Ib BHLH genes and of their two closest homologs to analyze whether their regulation would support a function in iron deWciency responses. We found that BHLH038, BHLH039, BHLH100 and BHLH101 (comprising a subgroup of BHLH Ib genes) were up regulated by iron deWciency in roots and leaves. Single insertion mutants had no visible phenotype and were capable of inducing root iron acquisition responses, presumably due to functional redundancy. SpeciWc metal treatments like nickel, high zinc or high copper resulted in induction of the four BHLH Ib genes whereas high iron, low copper and low zinc repressed gene expression. Induction of the four BHLH Ib genes was also found in multiple iron acquisition mutants including Wt. Ectopic activation of FIT did not suppress the four BHLH Ib genes. Split-root analyses using promoter-GUS lines showed that FIT and BHLH100 promoters were controlled by diVerent local and systemic signals involved in their regulation by iron. These results indicated that the four BHLH Ib genes were induced independently from FIT by conditions causing iron deWciency. Taken together, BHLH038, BHLH039, BHLH100 and BHLH101 function diVerently from FIT and may be involved in mediating a signal related to iron deWciencyinduced stress and/or internal iron homeostasis.

show abstract

Section: Analysis Of Subgroup Ib Bhlh Promoter-driven Gus Activitysupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Bhlh038 Bhlh039 Bhlh100 and Bhlh101 Gene Expression In Iromentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Hong-yu

Klatte

Jakoby

et al. 2007

Planta

224

202

View full text Add to dashboard Cite

show abstract

“…FRO2 encodes a major ferric chelate reductase controlling the amount of iron absorbed from the soil (Robinson et al, 1999;Connolly et al, 2003). IRT1 encodes a member of the ZIP (Zrt/Irt-like Protein) family of proteins used for the transport of Fe 2+ into cells across the plasma membrane (Vert et al, 2002). However, the IRT1 protein is not specific for iron.…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

View full text Add to dashboard Cite

These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

show abstract

Genetics of Metal Tolerance and Accumulation in Higher Plants

Datta

Sarkar

2004

Water Encyclopedia

View full text Add to dashboard Cite

Phytoremediation of metals is rapidly developing as a cost‐effective and environmentally friendly solution for cleaning up heavy‐metal‐contaminated sites. The most important requirements for effective phytoremediation are fast‐growing, high biomass plants that uptake and accumulate large amounts of toxic metals in their aboveground harvestable parts. A relatively small group of hyperaccumulator plants sequesters metals in their shoot tissues at high concentrations. In recent years, major scientific progress has been made in understanding the physiological mechanisms of metal uptake and transport in these plants. The majority of metal hyperaccumulators are slow growing and typically have low biomass, so bioengineering of nonaccumulators that have high biomass is essential for more effective phytoremediation. Biotechnology offers the opportunity to transfer hyperaccumulator phenotypes into fast‐growing, high biomass plants that could be highly effective in phytoremediation. However, comprehensive knowledge of the genetics of metal tolerance and accumulation is essential to design transgenic plants capable of phytoremediation. After an initial focus on plants with an immediate promise of application, research is now being directed at understanding the underlying mechanisms involved in heavy metal hyperaccumulation in plants. Once the rate‐limiting steps for uptake, translocation, and detoxification of metals in hyperaccumulator plants are identified, more informed construction of transgenic plants could become rather routine, which will help tremendously in more effective use of phytoremediation technology for cleaning up heavy‐metal‐contaminated sites.

show abstract

IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth

Cited by 1,426 publications

References 42 publications

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

Genetics of Metal Tolerance and Accumulation in Higher Plants

Contact Info

Product

Resources

About