Irreversible electropermeabilization of the human pathogen Candida albicans: an in-vitro experimental study

Novickij, Vitalij; Grainys, Audrius; Švedienė, Jurgita; Markovskaja, Svetlana; Paskevicius, Audrius; Novickij, Jurij

doi:10.1007/s00249-014-0996-3

Cited by 7 publications

(4 citation statements)

References 32 publications

(23 reference statements)

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“…However, the number of works focusing on the potential of EP for treatment of surface infections and multi-drug resistant bacterial strains is very limited. It is an emerging field and the feasibility of electroporation for treatment of surface bacterial infections, wound sterilization, and biofilm eradication was highlighted only recently and the first proof of concept was shown both in vitro and in vivo [ 25 , 26 , 27 ]. At the current state of knowledge, the additive effect of EP with antibiotics is unknown, however, in our previous work we have shown a proof of concept that electroporation can be used to sensitize drug-resistant Candida albicans , that is, PEF can be used to increase the sensitivity of pathogens to drugs to which the strains are initially resistant [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation

et al. 2018

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Treatment of bacteria-associated infections is complicated and antibiotic treatment alone is often inadequate to overcome biofilm infections. Physical methods allow overcoming this problem and propose solutions that are non-dependent on drug resistance. In this work, we investigated the feasibility of pulsed electric fields for sensitization of MRSA to common antibiotics. We analyzed the efficacy of inactivation of methicillin-resistant Staphylococcus aureus in 5–20 kV/cm electric field separately and in combination with gentamicin, doxycycline, ciprofloxacin, sulfamethoxazole, and vancomycin. Combined treatment allowed using up to 1000-fold smaller concentrations of antibiotics to induce the same inactivation of S. aureus.

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Section: Introductionmentioning

confidence: 99%

Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation

et al. 2018

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“…Novickij et al investigated combinations of electroporation with antifungal agents on C. albicans. Their results suggest that electroporation can be used as a tool to suppress C. albicans [24,25]. What is more, studies published in recent years provide cumulative evidence that cold atmospheric plasma (CAP) is a promising tool for membrane permeabilization and inactivation [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Effects of nanosecond pulsed electric fields (nsPEFs) on the human fungal pathogenCandida albicans:anin vitrostudy

Guo

Dang

Wang

et al. 2018

J. Phys. D: Appl. Phys.

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Candida albicans is the leading human fungal pathogen that causes many life-threatening infections. Notably, the current clinical trial data indicate that Candida species shows the emerging resistance to anti-fungal drugs. The aim of this study was to evaluate the antifungal effects of nanosecond pulsed electric fields (nsPEFs) as a novel drug-free strategy in vitro. In this study, we investigated the inactivation and permeabilization effects of C. albicans under different nsPEFs exposure conditions (100 pulses, 100 ns in duration, intensities of 20, 40 kV cm −1 ). Cell death was studied by annexin-V and propidium iodide staining. The changes of intracellular Ca 2+ concentration after nsPEFs treatment were observed using Fluo-4 AM. Results show that C. albicans cells and biofilms were both obviously inhibited and destroyed after nsPEFs treatment. Furthermore, C. albicans cells were significantly permeabilized after nsPEFs treatment. Additionally, nsPEFs exposure led to a large amount of DNA and protein leakage. Importantly, nsPEFs induced a field strength-dependent apoptosis in C. albicans cells. Further experiments revealed that Ca 2+ involved in nsPEFs induced C. albicans apoptosis. In conclusion, this proof-of-concept study provides a potential alternative drug-free strategy for killing pathogenic Candida species.

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“…The dynamics of the inactivation of T. rubrum in PEF are unknown and the synergistic effects of PEF with antifungal agents were not previously studied. Nevertheless, the PEF treatment has been successfully used for the inactivation of the Escherichia coli , Pseudomonas putida , Candida albicans , and Salmonella enterica …”

Section: Introductionmentioning

confidence: 99%

Controlled inactivation of Trichophyton rubrum using shaped electrical pulse bursts: Parametric analysis

Novickij

Grainys

Švedienė

et al. 2016

Biotechnology Progress

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The dermatophytes infect the skin by adherence to the epidermis followed by germination, growth, and penetration of the fungal hyphae within the cells. The aim of this study was to investigate the efficacy of the pulsed electric fields (PEF) of controlled inactivation of Trichophyton rubrum (ATCC 28188). In this work, we have used bursts of the square wave PEF pulses of different intensity (10-30 kV/cm) to induce the irreversible inactivation in vitro. The electric field pulses of 50 µs and 100 µs have been generated in bursts of 5, 10, and 20 pulses with repetition frequency of 1 Hz. The dynamics of the inactivation using different treatment parameters were studied and the inactivation map for the T. rubrum has been defined. Further, the combined effect of PEF with the antifungal agents itraconazole, terbinafine, and naftifine HCl was investigated. It has been demonstrated that the combined effect results in the full inactivation of T. rubrum colony. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1056-1060, 2016.

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Irreversible electropermeabilization of the human pathogen Candida albicans: an in-vitro experimental study

Cited by 7 publications

References 32 publications

Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation

Induction of Different Sensitization Patterns of MRSA to Antibiotics Using Electroporation

Effects of nanosecond pulsed electric fields (nsPEFs) on the human fungal pathogenCandida albicans:anin vitrostudy

Controlled inactivation of Trichophyton rubrum using shaped electrical pulse bursts: Parametric analysis

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