Abstract. We prove that the singular locus of rank 2 instanton sheaf E on P 3 which is not locally free has pure dimension 1. Moreover, we also show that the dual and double dual of E are isomorphic locally free instanton sheaves, and that the sheaves Ext 1 (E, O P 3 ) and E ∨∨ /E are rank 0 instantons. We also provide explicit examples of instanton sheaves of rank 3 and 4 illustrating that all of these claims are false for higher rank instanton sheaves.