Irradiation system of ions (H–Xe) for biological studies near the Bragg peak

Konishi, Teruaki; Yasuda, N.; Takeyasu, Akihiro; Ishizawa, Sachi; Fujisaki, Takashi; Matsumoto, K.; Furusawa, Yoshiya; Satô, Yukio; Hieda, Kotaro

doi:10.1063/1.2134408

Cited by 18 publications

(8 citation statements)

References 19 publications

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“…Irradiation was performed at the Medium Energy Beam (MEXP) course [ 16 ] at the Heavy-Ion Medical Accelerator in Chiba (HIMAC) [ 17 ] in the National Institute of Radiological Sciences (NIRS), Chiba, Japan. The cells were irradiated with a horizontal beam and set in perpendicular to the beam direction.…”

Section: Methodsmentioning

confidence: 99%

Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector

Kodaira¹,

Konishi²,

Kobayashi³

et al. 2014

Journal of Radiation Research

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The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion-induced biological effect. Single ion traversal through a cellular nucleus produces complex and massive DNA damage at a nanometer level, leading to cell inactivation, mutations and transformation. We present a novel approach that uses a fluorescent nuclear track detector (FNTD) for the simultaneous detection of the geometrical images of ion traversals and DNA damage in single cells using confocal microscopy. HT1080 or HT1080–53BP1-GFP cells were cultured on the surface of a FNTD and exposed to 5.1-MeV/n neon ions. The positions of the ion traversals were obtained as fluorescent images of a FNTD. Localized DNA damage in cells was identified as fluorescent spots of γ-H2AX or 53BP1-GFP. These track images and images of damaged DNA were obtained in a short time using a confocal laser scanning microscope. The geometrical distribution of DNA damage indicated by fluorescent γ-H2AX spots in fixed cells or fluorescent 53BP1-GFP spots in living cells was found to correlate well with the distribution of the ion traversals. This method will be useful for evaluating the number of ion hits on individual cells, not only for micro-beam but also for random-beam experiments.

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Section: Methodsmentioning

confidence: 99%

Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector

Kodaira¹,

Konishi²,

Kobayashi³

et al. 2014

Journal of Radiation Research

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“…CR-39 samples were irradiated to 60 MeV nitrogen ions at the medium beam energy course [23] of the HIMAC (Heavy Ion Medical Accelerator in Chiba) in NIRS (National Institute of Radiological Sciences). The exposed heavy ion density varied between 10 11 and 10 13 cm À2 .…”

Section: Methodsmentioning

confidence: 99%

Mass spectrometry analysis of etch products from CR-39 plastic irradiated by heavy ions

Kodaira¹,

Nanjo²,

Kawashima³

et al. 2012

Nuclear Instruments and Methods in Physics Research Section B:

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“…Heavy ions were imported to the Medium energy beam (MEXP) course 17,18) in Heavy Ion Medical Accelerator in Chiba (HIMAC) 18,19) at National Institute of Radiological Sciences (NIRS). The irradiation system of the MEXP course has been described in detail elsewhere.…”

Section: Irradiation Setupsmentioning

confidence: 99%

Visualization of Heavy Ion Tracks by Labeling 3'-OH Termini of Induced DNA Strand Breaks

Konishi¹,

Takeyasu²,

Natsume³

et al. 2011

JRR

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African green monkey kidney cells, CV-1, were irradiated with Carbon ions (LET: 735 keV/µm Argon ions (LET: 3,000 keV/µm) to visualize ion tracks through the cell nucleus by labeling the 3'-OH termini result of DNA strand breaks. The 3'-OH termini of DNA were labeled with BrdU-triphosphate catalyzed by TdT. This method of TUNEL (TdT-mediated dUTP Nick End labeling) is based on the specific binding of TdT to 3'-OH termini of DNA. Subsequent immuno-fluorescent staining with the primary monoclonal antibody against BrdU, followed by a secondary antibody of Alexa Fluor 488, was performed to visualize the BrdU labeled DNA termini. Images of the cell nuclei were acquired by confocal laser microscopy. When cell monolayers were irradiated perpendicularly with argon ions, induced DSBs in cell nuclei were identifiable as fluorescent spots. In another irradiation setup, when cells were irradiated at a small angle with incident argon ions, DNA strand breaks were detected as fluorescent stripes across the cell nucleus. These results demonstrate the induction of 3'-OH termini at sites of DNA strand breaks along Argon ion tracks.

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Irradiation system of ions (H–Xe) for biological studies near the Bragg peak

Cited by 18 publications

References 19 publications

Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector

Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector

Mass spectrometry analysis of etch products from CR-39 plastic irradiated by heavy ions

Visualization of Heavy Ion Tracks by Labeling 3'-OH Termini of Induced DNA Strand Breaks

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