Irradiation pretreatment enhances the therapeutic efficacy of platelet-membrane- camouflaged antitumor nanoparticles

Abstract

Backgroud: Cell membrane-based nanocarriers are promising candidates for delivering antitumor agents. The employment of a simple and feasible method to improve the tumor-targeting abilities of these systems is appealing for further application. Herein, we prepared a platelet membrane (PM)-camouflaged antitumor nanoparticle. The effects of irradiation pretreatment on tumor targeting of the nanomaterial and on its antitumor action were evaluated.Results: The biomimetic nanomaterial constructed by indocyanine green, poly(d,l-lactide-co-glycolide), and PM is termed PINPs@PM. A 4-Gy X-ray irradiation enabled the endocytic enhancement of PINPs@PM by 4T1 breast cancer cells. PINPs@PM produced hyperthermia and reactive oxygen species upon excitation by near-infrared irradiation, which were detrimental to the cytoplasmic lysosome and resulted in cell death. Irradiation pretreatment thus strengthened the antitumor activity of PINPs@PM in vitro. Mice experiments revealed that irradiation enhanced the tumor targeting capability of PINPs@PM in vivo. When the same dose of PINPs@PM was intravenously administered, irradiated mice had a better outcome than did mice without X-ray pretreatment. Conclusion: The study demonstrates an effective strategy combining irradiation pretreatment and PM camouflage to deliver antitumor nanoparticles, which may be instrumental for targeted tumor therapy.

Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection

“…Flow cytometry was performed as we have recently described. 38 Briefly, HK-2 was seeded into 6-well plates at a density of 2 × 10 5 cells per well and cultured overnight in DMEM containing 10% FBS. The cells were coincubated with 100 ng mL –1 S1 and D614G-S1 for 24 h in the absence and presence of NP (100, 50, and 10 μg mL –1 , based on the membrane protein) pretreatment.…”

Chiral nanomaterials for tumor therapy: autophagy, apoptosis, and photothermal ablation

“…Photothermal conversion then increases the temperature of the tumor tissue, thereby damaging tumor cells through protein denaturation and membrane disruption [ 74 , 75 ]. In recent years, several studies have demonstrated that the heat generated by nano-photothermal materials not only has the effect of directly killing tumor cells, it can also inhibit tumor metastasis [ 76 – 78 ]. In addition, nano-photothermal materials can also be used in bioimaging, and they may also be modified in such a way as to impart properties that may be beneficial to chemotherapy, radiotherapy, and immunotherapy, making them effective multifunctional diagnostic and therapeutic agents against tumors [ 79 , 80 ].…”

Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles

“…The resulting cell membrane camouflaged NPs preserve the physicochemical properties of the NP core while possessing the complex components of a natural cell membrane, which might endow the NPs with many desirable biological functions. Recently, a variety of cells except red blood cells (RBCs), including platelets, immune cells (e.g., macrophages), and cancer cells have been used to obtain membrane materials [26][27][28][29]. Compared with single cell membrane, hybrid cell membrane-coating approach can endow synthetic NPs with multifunctional and complex cell-like functions [30].…”