Irradiation-induced hippocampal neurogenesis impairment is associated with epigenetic regulation of bdnf gene transcription

Ji, Shengjun; Tian, Ye; Lu, Yi; Sun, Rui; Ji, Jianfeng; Zhang, Liyuan; Duan, Shumin

doi:10.1016/j.brainres.2014.06.035

Cited by 39 publications

(43 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the downregulation of EDNRA, a tumor suppressor, contributes to colorectal cancer progression (21). A decrease in the expression level of BDNF is associated with irradiation-induced hippocampal neurogenesis impairment in Sprague Dawley rats (39). Therefore, radiotherapy in rectal cancer may also cause side effects via regulating the aforementioned genes.…”

Section: Discussionmentioning

confidence: 99%

Identification of molecular characteristics induced by radiotherapy in rectal cancer based on microarray data

Chen

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. The present study aimed to reveal the molecular characteristics induced by radiotherapy in rectal cancer at the transcriptome level. Microarray data (ID, GSE26027) downloaded from the Gene Expression Omnibus database were re-analyzed to identify differentially expressed genes (DEGs) between rectal cancer tissues during and prior to radiotherapy. The DEGs were then inputted into the database for annotation, visualization and integrated discovery, an online tool to perform enrichment analyses, and into the search tool for the retrieval of interacting genes/proteins database to identify protein-protein interactions (PPIs). Subsequently, a PPI network was constructed, which was screened for densely connected modules. Furthermore, protein domain enrichment analysis was performed. In total, 690 DEGs, including 179 upregulated and 511 downregulated DEGs, were found in rectal cancer tissues during and prior to radiotherapy. The upregulated DEGs were significantly enriched in 'positive regulation of transport' and 'regulation of cardiac muscle contraction', while the downregulated DEGs were most markedly enriched in 'cell migration', 'cell-cell signaling', 'extracellular matrix organization' and 'blood vessel development', including prostaglandin-endoperoxide synthase 2, transforming growth factor β-induced, 68 kDa endothelin receptor type A, brain-derived neurotrophic factor, TIMP metallopeptidase inhibitor 1, and serpin family E member 1, which were the top 6 hub nodes in the PPI network. Furthermore, 2 protein domains were significantly enriched by PPI modules, including: The collagen triple helix repeat (CTHR) family members collagen type (COL) 5A2, COL9A3, COL6A3, COL21A1, COL5A3, COL11A1, COL7A1 and CTHR-containing-1; and the olfactory receptor family (OR) members OR7E24, OR7A17, OR6A2, OR1F1, OR10H3 and OR7A10. A total of 7 upregulated DEGs were characterized as tumor suppressor genes, and 8 downregulated DEGs were characterized as oncogenes. The biological processes or protein domains enriched by upregulated or downregulated DEGs may improve the understanding of molecular characteristics in response to radiotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of molecular characteristics induced by radiotherapy in rectal cancer based on microarray data

Chen

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

show abstract

“…However, the effect of dose on associative memory has not always been negative. A dosage of 2 Gy/ 1 f was used in Ji's research and Zhang's research, and did not induce any impairment of associative memory [35,51]. In Caceres's research, whole brain irradiation of 5 Gy/1f in Wistar rats improved the associative memory by increasing the latency to enter the dark compartment [34].…”

Section: Passive and Active Avoidance Testmentioning

confidence: 99%

“…The irradiation doses utilized ranged from 2 Gy/1f [35,51] to 30 Gy/1f [51] (BED=3.33 Gy and 330Gy respectively), among which 5 Gy/1 f [34,59] and 10 Gy/1 f [35,51,63] (BED=13.33 Gy and 43.33Gy respectively) were the most frequently used. Decreased latency to enter the dark compartment and increased latency to enter the light compartment indicated impaired associative memory.…”

Section: Passive and Active Avoidance Testmentioning

confidence: 99%

“…On the contrary, a dosage of 5 Gy/1 f in Oh's research impaired the associative memory of C57BL/6J mice 17 days post-irradiation by decreasing the latency to enter the dark compartment [59]. Larger doses, such as 10 Gy/5 f [63], 10 Gy/1 f [51], 36 Gy/ 8 f [50] and 30 Gy/1f [51] (BED=16.67 Gy, 43.33 Gy, 90 Gy and 330 Gy respectively), induced the impairment of associative memory by decreasing the latency to enter the dark compartment, except that 10 Gy/1 f and 20 Gy/1 f did not change the latency to enter the dark compartment after two months postirradiation [35]. Different fractionations may yield different results in the passive and active avoidance test.…”

Section: Passive and Active Avoidance Testmentioning

confidence: 99%

“…The decreased time spent in novel location indicates impaired cognitive function. As for the passive and active avoidance test, latency to enter the dark compartment and light compartment are the two most commonly used endpoints [50,51]. Decreased latency to enter the dark compartment and increased latency to enter the light compartment indicate impaired associative memory.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Current Utilization of Cognitive Tests in the Research of Radiation-Induced Cognitive Dysfunction in Rodent Models

Li¹,

Chen²,

Zhang³

2017

Brain Disord Ther

Self Cite

View full text Add to dashboard Cite

Recent clinical studies suggest that radiation-induced damage to the hippocampus plays a considerable role in the cognitive dysfunction of patients after cranial irradiation. In particular, deficits in learning, memory, and spatial processing observed in patients who received WBI are thought to be related to hippocampal injury [7]. The mechanisms of radiation-induced cognitive dysfunction are not yet fully understood. According to the available knowledge, radiation-induced cognitive dysfunction is hypothesized to result from dynamic interactions between multiple cell types: vascular and glial clonogens, neurogenesis, neural function and neuroinflammation [8][9][10]. Therefore, studies into the mechanisms and preventive measures of radiation-induced cognitive dysfunction are of paramount importance to decrease the side effects of WBI and increase the quality of life of patients. To achieve this goal, widely acknowledged animal models and universally utilized cognitive tests are the prerequisite and foundation. The Establishment of Rodent ModelsRodents, including mice and rats, are the most commonly utilized animal models in medical research given their genetic background, anatomical structure, operability, and relatively low cost of use [11]. Experimental data indicated that rodents showed similar anatomical AbstractWhole brain irradiation using low LET rays has remained the mainstay to treat some primary and metastatic brain tumors. Radiation-induced cognitive dysfunction is a progressive and irreversible late side effect after whole brain irradiation and inevitably decreases the quality of life of cancer survivors. To address this negative issue, many studies have been performed to explore the mechanisms of radiation-induced cognitive dysfunction and to develop efficacious preventive and treating measures. The prerequisite and foundation of implementing a persuasive and profound study to investigate radiation-induced cognitive dysfunction is the utilization of widely acknowledged animal models and universally applied cognitive tests. In this review, articles studying radiation-induced cognitive dysfunction from 2011 to 2016 were collected. The establishment of animal models and detailed utilization of cognitive tests were analyzed and summarized. This review summarized the general range of irradiation doses and time intervals utilized and the effects of these two factors on the results of cognitive tests. changes and physiopathological mechanisms to human beings after cranial irradiation [12]. In addition, the effects of radiation on rodents could be assessed over relatively short time periods-weeks to months rather than years to decades. Anatomical and functional changes of rodents after cranial irradiation are dependent on age, dose, and sex, which are compatible with the risk factors in human patients [13]. Given that these observations are representative of the effects seen in patients, the rodent model would enable the efficient study of mechanisms and treatments.

show abstract

Superoxide Dismutase Family of Enzymes in Brain Neurogenesis and Radioprotection

Nguyen

Srinivasan

Huang

2016

Oxidative Stress in Applied Basic Research and Clinical Practice

View full text Add to dashboard Cite

Irradiation-induced hippocampal neurogenesis impairment is associated with epigenetic regulation of bdnf gene transcription

Cited by 39 publications

References 39 publications

Identification of molecular characteristics induced by radiotherapy in rectal cancer based on microarray data

Identification of molecular characteristics induced by radiotherapy in rectal cancer based on microarray data

The Current Utilization of Cognitive Tests in the Research of Radiation-Induced Cognitive Dysfunction in Rodent Models

Superoxide Dismutase Family of Enzymes in Brain Neurogenesis and Radioprotection

Contact Info

Product

Resources

About