Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro

Zhu, Tingting; Yan, Wu; Zhou, Xiangyu; Yang, Yeming; Wang, Yao

doi:10.1007/s10103-019-02750-3

Cited by 28 publications

(19 citation statements)

References 37 publications

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“…It was thought that these stem cells took the path for cell differentiation. Thus, there was not any signi cant increase in cell viability [22]. On the contrary, triple blue light application in our study revealed successful outcomes in cell proliferation of keratinocytes at 5 J/cm 2 energy density, with a rate of nearly 150%.…”

Section: Discussioncontrasting

confidence: 63%

“…Generally, red-LEDs have been handled in clinics and scienti c researches [22], but the information for the use of blue, green, and orange-LEDs are limited. Even so, it is known that low energy blue, green, and orange LEDs can promote several biological effects on the tissue and cellular levels [16,17,22]. Thus, it was stated that orange light can be a promising wavelength to combat skin aging [17].…”

Section: Discussionmentioning

confidence: 99%

“…Although the proliferative effects at the wavelengths of the red and near-infrared region were proven in many studies [18][19][20], it is thought that there is a need for a design of a more e cient optical system to obtain consistent outcomes. In addition to this problem, the majority of the existing studies still evaluates the possible effect of the wavelengths in the range of 600 to 700 nm [21], but only the minor researches show the promise of other wavelengths in the visible spectrum, such as the wavelengths in the range of 400 to 600 nm [16,17,22].…”

Section: Discussionmentioning

confidence: 99%

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Portable Multicolor LED-Based System for the Photobiomodulation Therapy on Wound Healing Process In Vitro

Topaloğlu

Balkaya

Çevik

2021

Preprint

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Photobiomodulation is a practical and non-invasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600–700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460–475 nm as blue, 515–535 nm as green, and 585–595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and cell migration (scratch) assay, respectively. It was observed that orange-LEDs were designated as the most triggering wavelength in terms of cell proliferation. Also, it was revealed with this device that different wavelengths can reach the intended accelerated wound healing process, so this optical system will be an advantageous design for future practical photobiomodulation studies in vitro.

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Section: Discussioncontrasting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Portable Multicolor LED-Based System for the Photobiomodulation Therapy on Wound Healing Process In Vitro

Topaloğlu

Balkaya

Çevik

2021

Preprint

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“…A recent study showed that blue light irradiation inhibited gingiva-derived MSCs proliferation in 2D culture, as indicated by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]-test (MTT), and promoted osteogenesis. 82 According to the results of several viability tests, changes in the physiological activity of cells in the same hydrogel samples varied greatly [Figs. 4(c)-4(e)].…”

Section: Discussionmentioning

confidence: 99%

Beyond 2D: effects of photobiomodulation in 3D tissue-like systems

et al. 2020

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Significance: Currently, various scaffolds with immobilized cells are widely used in tissue engineering and regenerative medicine. However, the physiological activity and cell viability in such constructs might be impaired due to a lack of oxygen and nutrients. Photobiomodulation (PBM) is a promising method of preconditioning cells to increase their metabolic activity and to activate proliferation or differentiation. Aim: Investigation of the potential of PBM for stimulation of cell activities in hydrogels. Approach: Mesenchymal stromal cells (MSCs) isolated from human gingival mucosa were encapsulated in modified fibrin hydrogels with different thicknesses and concentrations. Constructs with cells were subjected to a single-time exposure to red (630 nm) and near-infrared (IR) (840 nm) low-intensity irradiation. After 3 days of cultivation, the viability and physiological activity of the cells were analyzed using confocal microscopy and a set of classical tests for cytotoxicity. Results: The cell viability in fibrin hydrogels depended both on the thickness of the hydrogels and the concentration of gel-forming proteins. The PBM was able to improve cell viability in hydrogels. The most pronounced effect was achieved with near-IR irradiation at the 840-nm wavelength. Conclusions: PBM using near-IR light can be applied for stimulation of MSCs metabolism and proliferation in hydrogel-based constructs with thicknesses up to 3 mm.

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“…Other studies have now also reported on osteo-and odonto-genic differentiation outcomes following irradiation by R/NIR light [10,[17][18][19][20][21][22]. Blue light (400-500 nm) has recently been shown capable of up-regulating the osteogenic potential of MSCs [23][24][25][26][27]. Even though the PBM mechanisms are not fully elucidated [9], the most widely accepted theory for the R/NIR PBM effects is in response to light absorption by cytochrome c oxidase (COX); which subsequently leads to stimulation of the respiratory chain and associated adenosine tri-phosphate (ATP) production [28].…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation of mineralisation in mesenchymal stem cells

Mohamad

Milward

Kuehne

et al. 2021

Photochem Photobiol Sci

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Mesenchymal stem cells (MSCs) and photobiomodulation (PBM) both offer significant therapeutic potential in regenerative medicine. MSCs have the ability to self-renew and differentiate; giving rise to multiple cellular and tissue lineages that are utilised in repair and regeneration of damaged tissues. PBM utilises light energy delivered at a range of wavelengths to promote wound healing. The positive effects of light on MSC proliferation are well documented; and recently, several studies have determined the outcomes of PBM on mineralised tissue differentiation in MSC populations. As PBM effects are biphasic, it is important to understand the underlying cellular regulatory mechanisms, as well as, provide accurate details of the irradiation conditions, to optimise and standardise outcomes. This review article focuses on the use of red, near-infra-red (R/NIR) and blue wavelengths to promote the mineralisation potential of MSCs; and also reports on the possible molecular mechanisms which underpin transduction of these effects. A variety of potential photon absorbers have been identified which are reported to mediate the signalling mechanisms, including respiratory chain enzymes, flavins, and cryptochromes. Studies report that R/NIR and blue light stimulate MSC differentiation by enhancing respiratory chain activity and increasing reactive oxygen species levels; however, currently, there are considerable variations between irradiation parameters reported. We conclude that due to its non-invasive properties, PBM may, following optimisation, provide an efficient therapeutic approach to clinically support MSC-mediated hard tissue repair. However, to optimise application, further studies are required to identify appropriate light delivery parameters, as well as elucidate the photo-signalling mechanisms involved.

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Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro

Cited by 28 publications

References 37 publications

Portable Multicolor LED-Based System for the Photobiomodulation Therapy on Wound Healing Process In Vitro

Portable Multicolor LED-Based System for the Photobiomodulation Therapy on Wound Healing Process In Vitro

Beyond 2D: effects of photobiomodulation in 3D tissue-like systems

Photobiomodulation of mineralisation in mesenchymal stem cells

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