Irradiated pollen for haploid production

Sestili, S.; Ficcadenti, N.

doi:10.1007/978-94-017-1860-8_15

Cited by 18 publications

(12 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plants obtained from pollen irradiated at 50 and 150 Gy suggest these are insufficient doses for pollen sterility (Froelicher et al, 2007). According to Sestili and Ficcadenti (1996), low levels of irradiation may damage only part of the generative nucleus while maintaining its capacity to fertilize the egg cell and lead to hybridization.…”

Section: Discussionmentioning

confidence: 99%

Production of Haploids in Persian Walnut through Parthenogenesis Induced by Gamma-irradiated Pollen

Grouh¹,

Vahdati²,

Lotfi³

et al. 2011

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

We report the first successful regeneration of haploid lines in persian walnut (Juglans regia) developed by in situ parthenogenesis followed by embryo rescue. Female flowers of cultivars Hartley and Pedro and two native Iranian selections (Z63 and Z67) were pollinated using pollen of selections Z53 and Z30 that had been irradiated with gamma rays at five doses (50, 150, 300, 600, and 900 Gy). Gamma-irradiated pollen induced fruit set and development of some parthenogenetic embryos. The immature embryos were excised 30 and 45 days after pollination, cultured in vitro, and then stratified for 30 days at 4 °C to overcome dormancy. Ploidy level of the resulting plantlets was determined by chromosome counting and flow cytometry. Haploid plants were obtained from ‘Hartley’, ‘Pedro’, Z63, and Z67 after pollination using pollen irradiated at 300 and 600 Gy. Plants obtained from pollen irradiated at 50 and 150 Gy were all diploid. Molecular marker analysis using four simple sequence repeat (SSR) markers also showed that all the diploid plants recovered were zygotic and no spontaneous double haploid plants were obtained in this work. Also, the haploid plantlets presented only one allele of their female parents. These profiles confirmed the parthenogenetic origin of the obtained haploid plants. The techniques used to induce haploid walnut plants by irradiated pollen were successful and could be used in breeding programs and accelerate genome analysis in this plant in which the genome size is approximately three times the size of the human genome.

show abstract

Section: Discussionmentioning

confidence: 99%

Production of Haploids in Persian Walnut through Parthenogenesis Induced by Gamma-irradiated Pollen

Grouh¹,

Vahdati²,

Lotfi³

et al. 2011

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…The poor hybrid embryo development in vitro (Fig. 4) could be explained that higher radiation may damage part of generative nucleus while maintaining its capacity to fertilize the egg cell, and lead to hybridization (Daskalov et al 1979;Sestili and Ficcadenti 1996). On all counts, further study is required to investigate the physiological and molecular mechanisms of pollen treatment with electrostatic fields and irradiation and the subsequent expression of this treatment on embryo germination and development.…”

Section: A Series Of Experiments On Overcoming Incompatibilities Betwmentioning

confidence: 96%

Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue

Wen

Chen

Liu

et al. 2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Embryo rescue technique was used successfully to produce interspecific hybrids by crossing peach (P. persica) as a female parent with apricot (P. armeniaca) and plum (P. salicica). In those crosses that had 'Yuhualu' or 'Zhonghuashoutao' as female parents, hybrid embryos aborted from the 7th or 8th week after pollination mainly due to post-pollination incompatibility. An embryo rescue protocol was established to rescue such embryos and recover hybrid plants. Modified half-strength MS medium containing 4 mg l -1 6-BA and 0.5 mg l -1 IBA produced up to 90% germination in the embryos. Modified MS medium with 1.0 mg l -1 6-BA and 1.0 mg l -1 IBA gave the highest bud induction and multiplication whereas modified MS medium containing 0.5 mg l -1 IAA and 0.2 mg l -1 NAA gave the best rooting percentage. All the hybrids obtained using this embryo rescue technique were verified using simple sequence repeat (SSR) markers. A series of pollen treatments were carried out to partially overcome pre-pollination incompatibility, and it was found accidentally that pollen treatment with electrostatic field not only improved pollen germination but also increased the multiplication coefficient of embryo-induced shoots.

show abstract

“…The pollination with irradiated pollen, at low irradiation levels, induces the growth of parthenocarpic fruit without zygotic embryos and the presence of some parthenogenic embryos of female gametophytic origin. Several factors can affect the haploid embryo generation rate, like the physiological stage of mother plants (Sauton, 1988a;Sestili and Ficcadenti, 1996), genotype (Cuny et al, 1992;Sauton, 1988b), pollen irradiation level (Cuny et al, 1992), or in vitro culture conditions (Sestili and Ficcadenti, 1996). Compared with other species of the Cucurbitaceae family, melon has a relatively better response than watermelon or zucchini to in situ-induced parthenogenesis (Przyborowski, 1996;Sauton and Dumas de Vaulx, 1987).…”

mentioning

confidence: 99%

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Gonzalo¹,

Claveria²,

Monforte³

et al. 2011

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Melon (Cucumis melo) is one of the principal vegetable crops for fresh market, for which a large number of breeding programs, oriented to generate inbred pure lines and hybrids, is established worldwide. The process to obtain and select these lines has been highly accelerated by the use of biotechnological techniques such as the generation of doubled haploid line (DHL) populations and molecular markers. Moreover, the use of DHLs in genetic studies is a useful tool because of their complete homozygosity and the permanent availability of plant material perpetuated by seed. In this work, the parthenogenetic response of 17 melon genotypes and the F1 hybrid PI 161375 × Spanish cultivar Piel de Sapo (PS) was studied considering three stages along the in vitro DHL generation process. The response of the analyzed melon cultivars was heterogeneous through the DHL generation with different limiting steps for each genotype. The response of the PI 161375 × PS hybrid was more similar to the male (PS) than the female parent (PI 161375), although the response of the maternal genotype was higher for some stages. This points to the important role of alleles from both parents in the different steps of the DHL generation process, and it could explain the identification of six genomic regions with distorted allelic segregation skewed toward PS or PI 161375. This hybrid was used to generate a population of 109 DHLs, the gametophytic origin of which was confirmed by flow cytometry and molecular markers.

show abstract

Irradiated pollen for haploid production

Cited by 18 publications

References 58 publications

Production of Haploids in Persian Walnut through Parthenogenesis Induced by Gamma-irradiated Pollen

Production of Haploids in Persian Walnut through Parthenogenesis Induced by Gamma-irradiated Pollen

Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Contact Info

Product

Resources

About