Irradiação gama para mutagênese in vitro em bananeira 'Terra Maranhão'

Pestana, Rosa Karla Nogueira; Amorim, Édson Perito; Silva, Sebastião de Oliveira e; Neto, Augusto Tulmann

doi:10.1590/s0100-204x2010001100015

Cited by 3 publications

(5 citation statements)

References 4 publications

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“…The dose of gamma irradiation used was 20 Gy, which was the dose previously selected in a sensitivity test (Pestana et al, 2010). Fifty buds were used as controls, which were sent to CENA/USP, without exposure to [ 60 Co].…”

Section: Gamma Irradiation Of In Vitro Budsmentioning

confidence: 99%

Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy

Reis¹,

Amorim²,

V.B.O.³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The objective of this study was to evaluate genetic variability and select putative mutants of Terra Maranhão plantain cultivar (AAB genome) subjected to gamma radiation based on agronomic data and inter simple sequence repeat molecular marker profiles using the Ward-MLM strategy. A total of 233 irradiated plants and 41 controls were assessed. The agronomic and molecular data were subjected to the Ward-MLM statistical algorithm in the SAS program. Cluster analysis was performed by the average distance method (UPGMA), based on the distance matrix of the Gower algorithm, and the cophenetic correlation coefficient calculated using the R software. The distance between the putative mutants ranged from 0.321 to 0.524, with an average distance of 0.426, and a cophenetic correlation coefficient of 0.79. Three putative mutants, which were selected based on the best agronomic traits and low height, will undergo further evaluation in the next stages of the banana breeding program at Embrapa. These results describe the first attempt of using combined data of Terra Maranhão plantain cultivar for the purpose of selecting mutants and (2015) assessing genetic variability.

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Section: Gamma Irradiation Of In Vitro Budsmentioning

confidence: 99%

Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy

Reis¹,

Amorim²,

V.B.O.³

et al. 2015

Genet. Mol. Res.

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show abstract

“…The dose used was 20 Gy, which was previously identified in the sensitivity test (Pestana et al, 2010). Fifty buds were used as controls, which were also sent to CENA/USP; however, they were not exposed to […”

Section: Gamma Irradiation Of In Vitro Budsmentioning

confidence: 99%

“…Therefore, in cases where the natural genetic variability is limited or there are difficulties related to the applications of traditional breeding methods, inducing mutations is tool that may help to overcome these barriers (Pestana et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Selection of putative Terra Maranhão plantain cultivar mutants obtained by gamma radiation

et al. 2015

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ABSTRACT. The aim of this study was to select putative Terra Maranhão plantain cultivar mutants obtained by gamma radiation, with good agronomic traits and short height. A total of 315 buds were irradiated in vitro with gamma rays in doses of 20 Gy and were subcultivated and evaluated in the field over 2 production cycles. The clones were evaluated to select the best 10% of the plants. Cultivation was undertaken at a spacing of 3 x 4 m, and fertilization was carried out according to the technical recommendations for the crop. A total of 111 irradiated plants and 41 controls were evaluated in the field. Among the irradiated plants selected, genotypes that exhibited reduced height were observed. The genotypes Irra 04, Irra 13, Irra 19, and Irra 21 exhibited a height of 3.6 m, which was below the mean value of the controls selected. Other irradiated genotypes selected such as Irra 14 and Irra 16, with a height of 3.65 m, are promising because, in addition to reduced height, they exhibited good bunch weight and shorter period to flowering in relation to the mean value of the controls, which is a significant factor for the next stages in breeding. These results confirm the possibility of inducing mutations in Terra type banana plants to obtain desirable agronomic traits and short height.

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“…Em situações como esta, e no caso onde existem limitações na variabilidade genética natural disponível, ou dificuldades relacionadas às aplicações de métodos tradicionais de melhoramento, uma ferramenta que pode superar estas barreiras é a indução de mutação (Pestana et al, 2010).…”

Section: Introductionunclassified

“…Em bananeira, alguns trabalhos têm sido realizados visando à geração de variabilidade genética para o melhoramento, a partir de irradiação com agentes físicos e ou químicos (Ho et al, 2001;Pestana et al, 2010). No entanto, apenas duas cultivares desenvolvidas por meio de raios gama foram registradas na Agência Internacional de Energia Atômica, a primeira a 'Klue Hom Thong KU1' que apresenta elevada massa do cacho e a 'Novaria' com ciclo precoce e qualidade dos frutos (Fao, 2011).…”

Section: Introductionunclassified

Caracterização agronômica de mutantes de bananeira obtidos por meio da radiação gama

et al. 2012

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A bananeira é uma das fruteiras de maior importância econômica, explorada quase exclusivamente por pequenos produtores. Constitui-se em fonte contínua de alimento e renda. Embora o Brasil figure como um dos maiores produtores de banana, a bananicultura nacional enfrenta sérios problemas nas fases de produção e pós-colheita, que limitam sua inserção no mercado internacional. Um dos fatores que levam a grandes perdas na produção é o tombamento resultante da altura elevada da planta das principais cultivares comerciais. Uma estratégia para a solução deste problema é a redução do porte por indução de mutação. O objetivo deste trabalho foi caracterizar mutantes de banana tipo Prata (cv´s Pacovan e Preciosa) irradiadas, durante dois ciclos de produção, visando à seleção de plantas com porte baixo e boas características agronômicas. Plantas in vitro das duas cultivares foram irradiadas com raios gamas nas doses de 20 Gy ('Pacovan' -200 mudas) e 30 Gy ('Preciosa' -200 mudas), subcultivadas por quatro vezes e posteriormente avaliadas em campo durante dois ciclos de produção. Foram selecionados quatro mutantes de cada cultivar com altura inferior à média de altura das testemunhas, após dois ciclos de avaliação. Observou-se que em alguns desses mutantes houve maior precocidade e maior massa do cacho quando comparados com as testemunhas. Pelos resultados, é possível selecionar plantas mutantes com características agronômicas superiores, tanto para a 'Pacovan' quanto para a 'Preciosa', submetidas à radiação gama.Palavras-chave: Musa spp., mutagênese in vitro, seleção de mutantes. Agronomic characterization of banana mutants obtained by gamma radiation AbstractBanana is one of the most economically important fruit, explored almost exclusively by small producers as a continuous source of food and income. Although Brazil is one of the main banana producers, the national banana production is undergoing serious problems especially in the phases of production and post-harvest limiting its participation in the international market. One of the main factors leading to great production losses is the toppling over due to the tall height of plants of main commercial cultivars. A strategy to solve this problem is reducing height by inducing mutation. The objective of the present work was to characterize irradiated Prata type banana mutants (cvs. Pacovan and Preciosa) during two production cycles in order to select short plants in height with good agronomic characteristics. In vitro plants of both cultivars were irradiated with gamma rays in the doses of 20 Gy (´Pacovan´, 200 plants) and 30 Gy (´Preciosa´, 200 plants) subcultivated four times and afterwards evaluated in the field during two production cycles. Four possible mutants were selected from each cultivar with height smaller than the average height of the controls after two evaluation cycles. It was observed that some of these mutants presented greater precocity and bunch weight compared to the controls. From the results obtained it is possible to select mutant plants with superio...

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Irradiação gama para mutagênese in vitro em bananeira 'Terra Maranhão'

Cited by 3 publications

References 4 publications

Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy

Genetic dissimilarity and selection of putative mutants of Terra Maranhão plantain cultivar using the Ward-MLM strategy

Selection of putative Terra Maranhão plantain cultivar mutants obtained by gamma radiation

Caracterização agronômica de mutantes de bananeira obtidos por meio da radiação gama

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