iRoot FM exerts an antibacterial effect on <i>Porphyromonas endodontalis</i> and improves the properties of stem cells from the apical papilla

Bi, Jianzhong; Liu, Y.; Liu, X. M.; Jiang, Li; Chen, Xu

doi:10.1111/iej.12923

Cited by 16 publications

(23 citation statements)

References 25 publications

(49 reference statements)

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“…A summary of the methodology used by the studies included in the review is shown in Table 1. Eight out of the ten included studies used human stem cells from the apical papilla (hSCAPs) from impacted immature third molars as their cell variant for analysis [32][33][34][35][36]38,39,41]. One of the studies used hSCAPs as their cell variant but did not specify its dental origin [40].…”

Section: Cell Variant and Originmentioning

confidence: 99%

“…The first category corresponds to the analyses measuring hSCAP cell viability, migration, and proliferation, for which a wide range of assays were used: flow cytometry [33,41], MTT assay [32,36], wound healing assay [32,35], transwell migration assay [32,40]; cell proliferation assays using trypan blue [33], WST-1 [40], Coulter counter [41], and BrdU labeling [32]; and cell viability assays using OZBlue [34], Alamar blue [37], CCK-8 [38], and XTT [39]. The second category was reserved for those analyses quantifying hSCAP expression of activity-related markers, majorly carried out using qRT-PCR [32][33][34][35][36]38,39,41], and followed by Western blot [32,38] and ELISA [38]. Analyses of the mineralization potential of hSCAPs represented the third category and were carried out exclusively using alizarin red staining (ARS) [32][33][34][35][36]38,41].…”

Section: Activity Analysismentioning

confidence: 99%

“…The second category was reserved for those analyses quantifying hSCAP expression of activity-related markers, majorly carried out using qRT-PCR [32][33][34][35][36]38,39,41], and followed by Western blot [32,38] and ELISA [38]. Analyses of the mineralization potential of hSCAPs represented the third category and were carried out exclusively using alizarin red staining (ARS) [32][33][34][35][36]38,41]. Activity analyses alongside with their duration and a description of the study associated with them are presented in Table 1.…”

Section: Activity Analysismentioning

confidence: 99%

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Viability and Stimulation of Human Stem Cells from the Apical Papilla (hSCAPs) Induced by Silicate-Based Materials for Their Potential Use in Regenerative Endodontics: A Systematic Review

et al. 2020

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Blood clot formation in the apical third of the root canal system has been shown to promote further root development and reinforcement of dentinal walls by the deposition of mineralized tissue, resulting in an advancement from traditional apexification procedures to a regenerative endodontic treatment (RET) for non-vital immature permanent teeth. Silicate-based hydraulic biomaterials, categorized as bioactive endodontic cements, emerged as bright candidates for their use in RET as coronal barriers, sealing the previously induced blood clot scaffold. Human stem cells from the apical papilla (hSCAPs) surviving the infection may induce or at least be partially responsible for the regeneration or repair shown in RET. The aim of this study is to present a qualitative synthesis of available literature consisting of in vitro assays which analyzed the viability and stimulation of hSCAPs induced by silicate-based hydraulic biomaterials. A systematic electronic search was carried out in Medline, Scopus, Embase, Web of Science, Cochrane and SciELO databases, followed by a study selection, data extraction, and quality assessment following the PRISMA protocol. In vitro studies assessing the viability, proliferation, and/or differentiation of hSCAPs as well as their mineralization potential and/or osteogenic, odontogenic, cementogenic and/or angiogenic marker expression in contact with commercially available silicate-based materials were included in the present review. The search identified 73 preliminary references, of which 10 resulted to be eligible for qualitative synthesis. The modal materials studied were ProRoot MTA and Biodentine. Both bioceramic materials showed significant positive results when compared to a control for hSCAP cell viability, migration, and proliferation assays; a significant up-regulation of hSCAP odontogenic/osteogenic marker (ALP, DSPP, BSP, Runx2, OCN, OSX), angiogenic growth factor (VEGFA, FIGF) and pro-inflammatory cytokine (IL-1α, IL-1β, IL-6, TNF-α) expression; and a significant increase in hSCAP mineralized nodule formation assessed by Alizarin Red staining. Commercially available silicate-based materials considered in the present review can potentially induce mineralization and odontogenic/osteogenic differentiation of hSCAPs, thus prompting their use in regenerative endodontic procedures.

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Section: Cell Variant and Originmentioning

confidence: 99%

Section: Activity Analysismentioning

confidence: 99%

Section: Activity Analysismentioning

confidence: 99%

See 1 more Smart Citation

Viability and Stimulation of Human Stem Cells from the Apical Papilla (hSCAPs) Induced by Silicate-Based Materials for Their Potential Use in Regenerative Endodontics: A Systematic Review

et al. 2020

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“…SCAP were primary cultured and characterized as described previously (Bi et al 2018). Informed consent was obtained from patients or parents (patients <18 years old).…”

Section: Primary Isolation and Culture Of Scapmentioning

confidence: 99%

“…This experiment was approved by the Ethics Committee of the School of Stomatology, China Medical University, Shenyang, China (201315). SCAP were primary cultured and characterized as described previously (Bi et al 2018). The apical papilla was digested with 3 mg mL À1 Collagenase I (Worthington Biochemical Co., Lakewood, CO, USA) and 4 mg mL À1 Dispase II (Boehringer Ingelheim, Mannheim, Germany).…”

Section: Primary Isolation and Culture Of Scapmentioning

confidence: 99%

Potential immunomodulatory effects of stem cells from the apical papilla on Treg conversion in tissue regeneration for regenerative endodontic treatment

Liu

et al. 2019

Int Endodontic J

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Aim To evaluate the expression of Foxp3‐positive lymphocytes around newly formed tissue after regenerative endodontic treatment (RET) in vivo and investigate the effects of stem cells from the apical papilla (SCAP) on the conversion of CD4+CD25− T cells to CD4+CD25+Foxp3+ regulatory T cells (Tregs) in vitro. Methodology Three 6‐month‐old beagles with nine doubled‐rooted premolars in each dog were randomly assigned to the RET group and the control group. RET was performed after apical periodontitis had been induced in the experimental immature teeth. Three months later, the expression of Foxp3 was detected in the histological sections by immunofluorescent staining. Human SCAP and CD4+CD25− T cells from mice spleens (1 : 1 and 1 : 5) were co‐cultured in cell–cell contact or in Transwells, respectively, for 24 and 72 h in vitro. The percentage of Tregs was evaluated by flow cytometry. The results were analysed using the Fisher's exact test and analysis of variance. P < 0.05 was regarded as statistically significant. Results Inflammatory cells were present with tissue regeneration in the RET group, and Foxp3‐positive T cells were enriched around the newly formed tissues. SCAP promoted Treg conversion after 72 h in vitro. Cell–cell contact played an important role after the 24 h co‐culture, whilst soluble factors were also involved after 72 h (P < 0.05). Conclusions SCAP promoted the conversion of pro‐inflammatory T cells to Tregs in vitro. Tregs were enriched around the regenerating tissues in the root canals after RET, which may create a suitable immune microenvironment for the differentiation of SCAP. This study provides an underlying mechanism for tissue regeneration during RET.

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In vitro and in vivo evaluation of iRoot BP Plus as a coronal sealing material for regenerative endodontic procedures

Yang,

Shen

et al. 2024

Clin Oral Invest

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Objectives To investigate in vitro effects of a nanoparticle bioceramic material, iRoot BP Plus, on stem cells from apical papilla (SCAP) and in vivo capacity to induce pulp-dentin complex formation. Materials and methods The sealing ability of iRoot BP Plus was measured via scanning electron microscopy (SEM). SCAP were isolated and treated in vitro by iRoot BP Plus conditioned medium, with mineral trioxide aggregate (MTA) conditioned medium and regular medium used as controls, respectively. Cell proliferation was assessed by BrdU labeling and MTT assay and cell migration was evaluated with wound healing and transwell assays. Osteo/odontogenic potential was evaluated by Alizarin red S staining and qPCR. Pulp-dentin complex formation in vivo was assessed by a tooth slice subcutaneous implantation model. Results iRoot BP Plus was more tightly bonded with the dentin. There was no difference in SCAP proliferation between iRoot BP Plus and control groups (P > 0.05). iRoot BP Plus had a greater capacity to elevated cell migration (P < 0.05) and osteo/odontogenic marker expression and mineralization nodule formation of SCAP compared with MTA groups (P < 0.05). Furthermore, the new continuous dentine layer and pulp-like tissue was observed in the iRoot BP Plus group in vivo. Conclusions iRoot BP Plus showed excellent sealing ability, promoted the migration and osteo/odontogenesis of SCAP and induced pulp-dentin complex formation without affecting the cell proliferation, which indicated iRoot BP Plus was a promising coronal sealing material in REPs. Clinical relevance The coronal sealing materials play crucial roles for the outcomes of REPs. This study showed that iRoot BP Plus has good coronal sealing and promote pulp-dentin complex formation compared with MTA, providing experimental evidences for the clinical application of iRoot BP Plus as a promising coronal seal material in REPs.

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iRoot FM exerts an antibacterial effect on Porphyromonas endodontalis and improves the properties of stem cells from the apical papilla

Abstract: iRoot FM exhibited excellent antibacterial activity against P. endodontalis and could improve the proliferation and differentiation of SCAP. The findings provide evidence that iRoot FM has potential as an intracanal medicament for endodontic procedures in immature permanent teeth.

Cited by 16 publications

References 25 publications

Viability and Stimulation of Human Stem Cells from the Apical Papilla (hSCAPs) Induced by Silicate-Based Materials for Their Potential Use in Regenerative Endodontics: A Systematic Review

Viability and Stimulation of Human Stem Cells from the Apical Papilla (hSCAPs) Induced by Silicate-Based Materials for Their Potential Use in Regenerative Endodontics: A Systematic Review

Potential immunomodulatory effects of stem cells from the apical papilla on Treg conversion in tissue regeneration for regenerative endodontic treatment

In vitro and in vivo evaluation of iRoot BP Plus as a coronal sealing material for regenerative endodontic procedures

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