Iron‐sulfur cluster carrier proteins involved in the assembly of <i>Escherichia coli</i> NADH:ubiquinone oxidoreductase (complex I)

Burschel, Sabrina; Decovic, Doris Kreuzer; Nuber, Franziska; Stiller, Marie; Hofmann, Maud; Zupok, Arkadiusz; Siemiatkowska, Beata; Górka, Michał; Leimkühler, Silke; Friedrich, Thorsten

doi:10.1111/mmi.14137

Cited by 29 publications

(24 citation statements)

References 82 publications

(101 reference statements)

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“…This in turn is necessary for the function of the TCA cycle and the glyoxylate shunt to produce amino acids . The growth monitoring during 24 h showed that the Δ ndh strain had no growth defect compared to the parental strain, as observed before . In contrast, both the Δ nuoC strain and the nuoC+D strain did not grow within the first 16 h of the experiment, while eventually the Δ nuoC strain showed a slight increase in cell density (Fig.…”

Section: Resultssupporting

confidence: 75%

“…The functionality of complex I was initially tested by monitoring the NADH oxidase activity, which is catalysed by complex I and the cytochrome bo 3 and cytochrome bd oxidases. The strain Dndh, lacking the alternative NADH dehydrogenase, showed an activity of 0.2 UÁmg À1 (Table 5), which also has been observed in a previous study [31]. The negative control strain lacking complex I (strain DnuoC-L) had only a residual oxidase activity of 4%.…”

Section: Removal or Separation Of Nuoc Causes Complete Loss Of Complesupporting

confidence: 85%

“…The sucrose gradients were prepared and NADH/ferricyanide oxidoreductase activity was determined basically as described [31]. Briefly, membrane extracts from cells grown under the appropriate growth conditions were prepared after cell rupture with an Emulsiflex (EF-C5; Avestin Europe, Mannheim, Germany) in the presence of PMSF (0.1 mM) and DNase at 160 000 g. The sediment was resuspended in buffer A (50 mM MES/NaOH, 50 mM NaCl) with 1% (w/v) DDM and 5 mM MgCl 2 .…”

Section: Sucrose Gradient Centrifugationmentioning

confidence: 99%

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The N‐terminal domains of the paralogous HycE and NuoCD govern assembly of the respective formate hydrogenlyase and NADH dehydrogenase complexes

et al. 2020

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Formate hydrogenlyase (FHL) is the main hydrogen‐producing enzyme complex in enterobacteria. It converts formate to CO2 and H2 via a formate dehydrogenase and a [NiFe]‐hydrogenase. FHL and complex I are evolutionarily related and share a common core architecture. However, complex I catalyses the fundamentally different electron transfer from NADH to quinone and pumps protons. The catalytic FHL subunit, HycE, resembles NuoCD of Escherichia coli complex I; a fusion of NuoC and NuoD present in other organisms. The C‐terminal domain of HycE harbours the [NiFe]‐active site and is similar to other hydrogenases, while this domain in NuoCD is involved in quinone binding. The N‐terminal domains of these proteins do not bind cofactors and are not involved in electron transfer. As these N‐terminal domains are separate proteins in some organisms, we removed them in E. coli and observed that both FHL and complex I activities were essentially absent. This was due to either a disturbed assembly or to complex instability. Replacing the N‐terminal domain of HycE with a 180 amino acid E. coli NuoC protein fusion did not restore activity, indicating that the domains have complex‐specific functions. A FHL complex in which the N‐ and C‐terminal domains of HycE were physically separated still retained most of its FHL activity, while the separation of NuoCD abolished complex I activity completely. Only the FHL complex tolerates physical separation of the HycE domains. Together, the findings strongly suggest that the N‐terminal domains of these proteins are key determinants in complex assembly.

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Section: Resultssupporting

confidence: 75%

Section: Removal or Separation Of Nuoc Causes Complete Loss Of Complesupporting

confidence: 85%

Section: Sucrose Gradient Centrifugationmentioning

confidence: 99%

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The N‐terminal domains of the paralogous HycE and NuoCD govern assembly of the respective formate hydrogenlyase and NADH dehydrogenase complexes

et al. 2020

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“…NADH (Nicotinamide adenine dinucleotide) reacts with free radicals to inhibit lipid peroxidation and protect mitochondrial membranes and mitochondrial function. Studies have found that NADH can reduce the oxidative stress of cells caused by various factors such as radiation, drugs, toxic substances, strenuous exercise, ischemia, etc., thereby protecting vascular endothelial cells, liver cells, cardiomyocytes, fibroblasts, and neurons [23]. Research by He et al showed that iTRAQ technology identified 212 different proteins in duck meat under heat stress conditions, of which 84% of down-regulated proteins involved enzymes that regulated the redox reaction process, such as COXs, NADH metabolism, and ATP synthesis/binding related enzymes [24].…”

Section: Discussionmentioning

confidence: 99%

Proteomic study of hypothalamus in pigs exposed to heat stress

Yong

et al. 2020

BMC Vet Res

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Background: With evidence of warming climates, it is important to understand the effects of heat stress in farm animals in order to minimize production losses. Studying the changes in the brain proteome induced by heat stress may aid in understanding how heat stress affects brain function. The hypothalamus is a critical region in the brain that controls the pituitary gland, which is responsible for the secretion of several important hormones. In this study, we examined the hypothalamic protein profile of 10 pigs (15 ± 1 kg body weight), with five subjected to heat stress (35 ± 1°C; relative humidity = 90%) and five acting as controls (28 ± 3°C; RH = 90%). Result: The isobaric tags for relative and absolute quantification (iTRAQ) analysis of the hypothalamus identified 1710 peptides corresponding to 360 proteins, including 295 differentially expressed proteins (DEPs), 148 of which were up-regulated and 147 down-regulated, in heat-stressed animals. The Ingenuity Pathway Analysis (IPA) software predicted 30 canonical pathways, four functional groups, and four regulatory networks of interest. The DEPs were mainly concentrated in the cytoskeleton of the pig hypothalamus during heat stress. Conclusions: In this study, heat stress significantly increased the body temperature and reduced daily gain of body weight in pigs. Furthermore, we identified 295 differentially expressed proteins, 147 of which were down-regulated and 148 up-regulated in hypothalamus of heat stressed pigs. The IPA showed that the DEPs identified in the study are involved in cell death and survival, cellular assembly and organization, and cellular function and maintenance, in relation to neurological disease, metabolic disease, immunological disease, inflammatory disease, and inflammatory response. We hypothesize that a malfunction of the hypothalamus may destroy the host physical and immune function, resulting in decreased growth performance and immunosuppression in heat stressed pigs.

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“…NADH (Nicotinamide adenine dinucleotide) reacts with free radicals to inhibit lipid peroxidation and protect mitochondrial membranes and mitochondrial function. Studies have found that NADH can reduce the oxidative stress of cells caused by various factors such as radiation, drugs, toxic substances, strenuous exercise, ischemia, etc., thereby protecting vascular endothelial cells, liver cells, cardiomyocytes, broblasts, and neurons [23]. Research by He et al showed that iTRAQ technology identi ed 212 different proteins in duck meat under heat stress conditions, of which 84% of downregulated proteins involved enzymes that regulated the redox reaction process, such as COXs, NADH metabolism, and ATP synthesis/binding related enzymes [24].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Study of Hypothalamus in Pigs Exposed to Heat Stress

Yong

et al. 2020

Preprint

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Background : With evidence of warming climates, it is important to understand the effects of heat stress in farm animals in order to minimize production losses. Studying the changes in the brain proteome induced by heat stress may aid in understanding how heat stress affects brain function. The hypothalamus is a critical region in the brain that controls the pituitary gland, which is responsible for the secretion of several important hormones. In this study, we examined the hypothalamic protein profile of 10 pigs (15 ± 1 kg body weight), with five subjected to heat stress (35 ± 1 °C; relative humidity = 90%) and five acting as controls (28 ± 3°C; RH = 90%). Result: The isobaric tags for relative and absolute quantification (iTRAQ) analysis of the hypothalamus identified 1710 peptides corresponding to 360 proteins, including 295 differentially expressed proteins (DEPs), 148 of which were up-regulated and 147 down-regulated, in heat-stressed animals. The Ingenuity Pathway Analysis (IPA) software predicted 30 canonical pathways, four functional groups, and four regulatory networks of interest. The DEPs were mainly concentrated in the cytoskeleton of the pig hypothalamus during heat stress. Conclusions: In this study, heat stress significantly increased the body temperature and reduced daily gain of body weight in pigs. Furthermore, we identified 295 differentially expressed proteins, 147 of which were down-regulated and 148 up-regulated in hypothalamus of heat stressed pigs. The IPA showed that the DEPs identified in the study are involved in cell death and survival, cellular assembly and organization, and cellular function and maintenance, in relation to neurological disease, metabolic disease, immunological disease, inflammatory disease, and inflammatory response. We hypothesize that a malfunction of the hypothalamus may destroy the host physical and immune function, resulting in decreased growth performance and immunosuppression in heat stressed pigs.

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Iron‐sulfur cluster carrier proteins involved in the assembly of Escherichia coli NADH:ubiquinone oxidoreductase (complex I)

Cited by 29 publications

References 82 publications

The N‐terminal domains of the paralogous HycE and NuoCD govern assembly of the respective formate hydrogenlyase and NADH dehydrogenase complexes

The N‐terminal domains of the paralogous HycE and NuoCD govern assembly of the respective formate hydrogenlyase and NADH dehydrogenase complexes

Proteomic study of hypothalamus in pigs exposed to heat stress

Proteomic Study of Hypothalamus in Pigs Exposed to Heat Stress

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