Iron stores assessment in alcoholic liver disease

Matos, Luís Costa; Batista, Paulo; Monteiro, Nuno P.; Ribeiro, João; Cipriano, Maria Augusta; Henriques, Pedro; Girão, Fernando; Carvalho, Armando

doi:10.3109/00365521.2013.781217

Cited by 22 publications

(6 citation statements)

References 29 publications

(30 reference statements)

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“…Excessive iron intake and iron overload are harmful for hepatic homeostasis. [ 62,63 ] The iron over‐intake risk should be assessed for the supplement of Lf, as an iron‐binding protein. The iron saturation rate of native bovine Lf is 15–20%, corresponding to 0.25 mg of iron in per gram of Lf.…”

Section: Discussionmentioning

confidence: 99%

Daily Dose of Bovine Lactoferrin Prevents Ethanol‐Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota

Liu

Qian

Yang

et al. 2021

Molecular Nutrition Food Res

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Scope Lactoferrin (Lf) possess a protective potential to liver, but whether it can prevent alcoholic liver injury (ALI) remains unclear. Methods and Results Four groups of male C57BL/6J mice are fed with different diets, namely, AIN‐93G diet for control (CON) and ethanol (EtOH) groups, and AIN‐93G diet with 0.4% and 4% casein replaced by Lf for low‐dose Lf (LLf) and high‐dose Lf (HLf) groups, respectively. ALI is induced by giving 20% ethanol ad libitum combined with four “binges”. Lf can remarkably decrease EtOH‐induced mortality. Lf promotes aldehyde dehydrogenase‐2 (ALDH2) expression and suppressing cytochrome P450 2E1 (CYP2E1) overexpression, resulting in the reduced hepatic superoxide and inflammation levels, which ultimately leads to the hepatic injury alleviation. However, HLf increases acetyl‐CoA carboxylase and fatty acid synthase protein levels, which suggests that excessive intake may weaken the beneficial effects of Lf. Moreover, LLf increases the relative abundances of Akkermansia and Lactobacillus. Additionally, the study shows that Lf likely exerts action in its digestive product forms rather than intact Lf molecular in normal condition. Conclusion LLf can ameliorate ALI, which is associated with the regulation of hepatic alcohol metabolism and the modulation of gut microbiota. However, excessive Lf intake may result in a diminished benefit.

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Section: Discussionmentioning

confidence: 99%

Daily Dose of Bovine Lactoferrin Prevents Ethanol‐Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota

Liu

Qian

Yang

et al. 2021

Molecular Nutrition Food Res

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“…Today, Deugnier and Turlin’s classification of Perls’ staining is the best-validated biochemical method for determining liver iron in tissue specimens, in both hemochromatotic and nonhemochromatotic iron-overload disorders, but it is still mainly used for research purposes because of its complexity [20, 26, 27, 28]. Validation of Scheuer’s classification by comparison with biochemical determination of liver iron content was, until recently, based mainly on a report published in the 1990s by Scheuer’s group in thalassemia major patients treated with the chelator desferrioxamine, but recent publications have also shown its validity in alcoholic liver disease and sickle cell disease [29, 30, 31]. It is also noteworthy that recent publications on the epidemiology of iron-overload diseases in African-American and cirrhotic explants of alpha1-antitrypsin deficiency used Perls’ histological grading as the mainstay of their research [32, 33].…”

Section: Discussionmentioning

confidence: 99%

Signal-intensity-ratio MRI accurately estimates hepatic iron load in hemodialysis patients

Rostoker

Laroudie²,

Blanc

et al. 2017

Heliyon

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BackgroundIron overload, diagnosed by means of magnetic resonance imaging (MRI), is an increasingly recognized disorder in hemodialysis patients. Specific MRI protocols have been shown to provide a reliable estimation of tissue iron content in non-renal patient populations but have not been validated in dialysis patients. Such validation studies require liver biopsy for histological comparison, but this invasive and risky procedure raises ethical concerns, especially regarding frail patients with end-stage renal disease.Materials and methodsWe compared in a pilot study Scheuer’s histological classification and Deugnier and Turlin’s histological classification of iron overload (Perls staining) with signal-intensity-ratio MRI values obtained with the Rennes University algorithm in 11 hemodialysis patients in whom liver biopsy was formally indicated for their medical follow-up.ResultsFor Scheuer’s histological classification, the Wilcoxon non-parametric matched-pairs test showed no significant difference in the ranking of iron overload by the two methods eg histology and MRI (sum of ranks = 1.5; p = 1). The MRI and Scheuer’s histological classifications were tightly correlated (rho = 0.866, p = 0.0035, Spearman’s coefficient), as were the absolute liver iron concentrations (LIC) at MRI (rho = 0.860, p = 0.0013, Spearman’s coefficient). The absolute liver iron concentrations at MRI were also highly correlated with Deugnier and Turlin’s histological scoring (rho = 0.841, p = 0.0033, Spearman’s coefficient).ConclusionsThis pilot study shows that liver iron determination based on signal-intensity-ratio MRI (Rennes University algorithm) very accurately identifies iron load in hemodialysis patients, by comparison with liver histology.

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“…Under normal conditions, levels of serum ferritin show a close correlation with iron stores in liver biopsy samples [ 34 ], the “gold standard” for measuring the amount of iron in the body. However, serum ferritin levels can be profoundly affected by the presence of inflammation, since serum ferritin is an acute-phase protein.…”

Section: Ferritin: the Ubiquitous Iron Storage Proteinmentioning

confidence: 99%

Limitations of Serum Ferritin in Diagnosing Iron Deficiency in Inflammatory Conditions

Dignaß

Farrag

Stein

2018

International Journal of Chronic Diseases

181

213

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Patients with inflammatory conditions such as inflammatory bowel disease (IBD), chronic heart failure (CHF), and chronic kidney disease (CKD) have high rates of iron deficiency with adverse clinical consequences. Under normal circumstances, serum ferritin levels are a sensitive marker for iron status but ferritin is an acute-phase reactant that becomes elevated in response to inflammation, complicating the diagnosis. Proinflammatory cytokines also trigger an increase in hepcidin, which restricts uptake of dietary iron and promotes sequestration of iron by ferritin within storage sites. Patients with inflammatory conditions may thus have restricted availability of iron for erythropoiesis and other cell functions due to increased hepcidin expression, despite normal or high levels of serum ferritin. The standard threshold for iron deficiency (<30 μg/L) therefore does not apply and transferrin saturation (TSAT), a marker of iron availability, should also be assessed. A serum ferritin threshold of <100 μg/L or TSAT < 20% can be considered diagnostic for iron deficiency in CHF, CKD, and IBD. If serum ferritin is 100–300 μg/L, TSAT < 20% is required to confirm iron deficiency. Routine surveillance of serum ferritin and TSAT in these at-risk groups is advisable so that iron deficiency can be detected and managed.

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Iron stores assessment in alcoholic liver disease

Cited by 22 publications

References 29 publications

Daily Dose of Bovine Lactoferrin Prevents Ethanol‐Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota

Daily Dose of Bovine Lactoferrin Prevents Ethanol‐Induced Liver Injury and Death in Male Mice by Regulating Hepatic Alcohol Metabolism and Modulating Gut Microbiota

Signal-intensity-ratio MRI accurately estimates hepatic iron load in hemodialysis patients

Limitations of Serum Ferritin in Diagnosing Iron Deficiency in Inflammatory Conditions

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