Iron regulation of chloroplast photosynthetic function: Mediation of PS I development

Pushnik, James C.; Miller, Gene W.

doi:10.1080/01904168909363962

Cited by 74 publications

(34 citation statements)

References 16 publications

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“…The expression of Fdh in Fe-deficient barley roots suggests that Fe deficiency caused changes similar to the ones caused by anoxia through changes in heme biosynthesis. For example, Fe regulates the biosynthesis of ␦-aminolevulinic acid (by ␦-aminolevulinic acid synthase; Pushnik and Miller, 1989) and protoporphyrinogen IX (by co-proporphyrinogen III oxidase). These are the common precursors of protoporphyrin IX, from which chlorophyll a in the chloroplasts and heme in the mitochondria are synthesized.…”

Section: Discussionmentioning

confidence: 99%

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

et al. 1998

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To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel electrophoresis. Peptide sequence analysis of induced proteins revealed that formate dehydrogenase (FDH), adenine phosphoribosyltransferase, and the Ids3 gene product (for Fe deficiency-specific) increased in Fe-deficient roots. FDH enzyme activity was detected in Fe-deficient roots but not in Fe-sufficient roots. A cDNA encoding FDH (Fdh) was cloned and sequenced. Fdh expression was induced by Fe deficiency. Fdh was also expressed under anaerobic stress and its expression was more rapid than that induced by Fe deficiency. Thus, the expression of Fdh observed in Fe-deficient barley roots appeared to be a secondary effect caused by oxygen deficiency in Fe-deficient plants.In Fe-deficient calcareous soils graminaceous plants secrete mugineic acid family phytosiderophores, which are natural Fe chelators, from the roots (Takagi, 1976) to solubilize Fe required for plant growth. This Fe-acquisition mechanism in graminaceous plants is called strategy II and in nongraminaceous plants it is called strategy I (Takagi et al., 1984;Marschner et al., 1986). The pathway of the biosynthesis of mugineic acid family phytosiderophores has been established Nishizawa, 1987, 1989;Shojima et al., 1989Shojima et al., , 1990Mori et al., 1990;Ma and Nomoto, 1993). Among the enzymes involved in this biosynthetic pathway, Higuchi et al. (1994Higuchi et al. ( , 1996 purified nicotianamine synthase and Kanazawa et al. (1994) purified nicotianamine aminotransferase. Comparison of 2D profiles of proteins in barley (Hordeum vulgare L.) roots under Fesufficient and Fe-deficient conditions (Suzuki et al., 1995(Suzuki et al., , 1997 allowed us to identify a 36-kD protein that was specifically induced by Fe deficiency. In addition, several genes related to the Fe-deficiency response have been reported: Ids1 (Okumura et al., 1991), Ids2 (Okumura et al., 1994), and Ids3 (Nakanishi et al., 1993. In this study, we characterized several other proteins induced by Fedeficiency stress in barley roots, one of which was identified as FDH. FDH was induced not only by Fe deficiency but also by anaerobic stress. The relationship between Fe deficiency and anaerobic stress in barley roots is discussed. MATERIALS AND METHODS Plant Material and Growth ConditionsSeeds of barley (Hordeum vulgare L. cv Ehimehadaka no. 1) were germinated at room temperature on paper towels soaked with distilled water. Plants were transferred 4 d after germination to a plastic net floating on tap water at pH 5.5 in a greenhouse under natural light. On d 10, plants were transferred to a continuously aerated nutrient solution of the following composition: 0.7 mm K 2 SO 4 , 0.1 mm KCl, 0.1 mm KH 2 PO 4 , 2.0 mm Ca(NO 3 ) 2 , 0.5 mm MgSO 4 , 10 m H 3 BO 3 , 0.5 m MnSO 4 , 0.2 m CuSO 4 , 0.5 m ZnSO 4 , 0.01 m (NH 4 ) 6 Mo 7 O 24 , and 0.1 mm Fe-EDTA. The pH of the culture solution was adjusted to 5.5 da...

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Section: Discussionmentioning

confidence: 99%

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

et al. 1998

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“…A low drainage Also a low bioavailability of micronutrients can directly reduce the chlorophyll biosynthesis. Indeed, Fe is involved directly in electron transport reactions and is essential for the production of chlorophyll 21,22 as cofactor of cytochromes (electron transfer proteins) required for chlorophyll synthesis. Copper is also directly involved in electron transport reactions as it is cofactor of photosynthetic electron transfer protein (plastocyanin) 23,24 .…”

Section: Resultsmentioning

confidence: 99%

Morphological and Physiological Responses of Barley Plants Grown on Soils Characterised by Metal Toxicity and Metal Deficiency

et al. 2007

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Aim of this work was to investigate which are the effects on barley crops grown on two different soils: a soil lacking in Cu, an essential micronutrient (A) and a naturally polluted soil rich in lead, zinc, copper (B). In particular we investigated the relationship between some ecophysiological parameters such as biomass, chlorophyll concentration and guaiacolo peroxidase activity and the chemical-physical properties of the soils like pH, organic matter and heavy metal content. Because metals uptake by plants is strongly correlated with the bioavailable fraction rather then their total amount in a soil, we have measured also metal exchangeable forms, using a single extraction method (MgCl2 as extractant). Plants grown on soil B showed a metal content higher than background limits, whereas plants grown on soil A were characterised by a background Fe and Zn concentrations and by a tolerant Pb concentration. Conversely, Cu content in tissues of plants grown in soil A is found to be under the background limits. Copper-deficiency plants present chlorotic leaves followed by a reduced clorophyll content, while plants grown on metals contaminated soil showed an increase of peroxidase activity.

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“…Iron is also essential for the biosynthesis of chlorophyll (Pushnik and Miller, 1989). It plays an important role in biological redox systems and enzyme activation.…”

Section: +mentioning

confidence: 99%

Nutrient Stress

Reddy

2006

Physiology and Molecular Biology of Stress Tolerance in Plants

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Iron regulation of chloroplast photosynthetic function: Mediation of PS I development

Cited by 74 publications

References 16 publications

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

Morphological and Physiological Responses of Barley Plants Grown on Soils Characterised by Metal Toxicity and Metal Deficiency

Nutrient Stress

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