Iron reducing sludge as a source of electroactive bacteria: assessing iron reduction in biofilm bacteria, planktonic cells and isolates from a microbial fuel cell

González-Paz, J. R.; Becerril-Varela, Karina; Guerrero–Barajas, Claudia

doi:10.1007/s00203-022-03253-6

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…The obtained results were higher compared to the results obtained by González-Paz et al (2022) [54], who used iron-reducing bacteria with a mineral medium, obtaining the highest voltage (0.3 V) using a salt bridge. Huang et al (2023) [55] evaluated the use of a consortium of filamentous sulphate-reducing microorganisms from sediments, applied in a sediment fuel cell with an inorganic substrate source, obtaining a maximum voltage of 100 mV.…”

Section: Voltage Productioncontrasting

confidence: 64%

Exploration of In Vitro Voltage Production by a Consortium of Chemolithotrophic Microorganisms Using Galena (PbS) as a Sulphur Source

Gaucin Gutiérrez,

Rojas-Contreras,

Zazueta-Álvarez

et al. 2024

Clean Technol.

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Sulphur plays a fundamental role in the biological processes of chemolithotrophic microorganisms. Due to the redox characteristics of sulphur, microorganisms use it for metabolic processes. Such is the case of the dissimilatory processes in the anaerobic respiration of reducing microorganisms. The production of electrical energy from the metabolism of native microorganisms using sulphur as substrate from inorganic mineral sources in the form of Galena (PbS) was achieved using MR mineral medium with 15% (w/v) of PbS mineral concentrate. At 400 h of growth, the highest voltage produced in an experimental unit under anaerobic conditions was 644 mV. The inoculum was composed of microorganisms with spiral morphology, and at the final stages of energy production, the only microorganism identified was Bacillus clausii. This microorganism has not been reported in bioelectrochemical systems, but it has been reported to be present in corrosive environments and reducing anoxic environments.

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Section: Voltage Productioncontrasting

confidence: 64%

Exploration of In Vitro Voltage Production by a Consortium of Chemolithotrophic Microorganisms Using Galena (PbS) as a Sulphur Source

Gaucin Gutiérrez,

Rojas-Contreras,

Zazueta-Álvarez

et al. 2024

Clean Technol.

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“…Wu et al (2018) managed to remove 78.8% of Cu in an MFC when using sludge as a substrate, mentioning that the decrease in Cu was due to the reductive reactions in the oxidation process of organic compounds, where the substrate was oxidized by the microorganisms electrochemically in order to produce electrons and protons [53]. Gonzalez et al (2022) reduced the percentage of iron by 60% using sludge as a substrate in microbial fuel cells, mentioning that this reduction was due to the presence of planktonic cells [54]. Becerril et al (2021) achieved iron reductions in the range of 80-90% in their MFC when using wastewater as a substrate, mentioning that the acetate present in their samples was used by the exoelectrogenic bacteria as a source of preferred carbon [55].…”

Section: Resultsmentioning

confidence: 99%

Reduction of Toxic Metal Ions and Production of Bioelectricity through Microbial Fuel Cells Using Bacillus marisflavi as a Biocatalyst

Segundo,

De La Cruz-Noriega,

Luis

et al. 2024

Molecules

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Industrialization has brought many environmental problems since its expansion, including heavy metal contamination in water used for agricultural irrigation. This research uses microbial fuel cell technology to generate bioelectricity and remove arsenic, copper, and iron, using contaminated agricultural water as a substrate and Bacillus marisflavi as a biocatalyst. The results obtained for electrical potential and current were 0.798 V and 3.519 mA, respectively, on the sixth day of operation and the pH value was 6.54 with an EC equal to 198.72 mS/cm, with a removal of 99.08, 56.08, and 91.39% of the concentrations of As, Cu, and Fe, respectively, obtained in 72 h. Likewise, total nitrogen concentrations, organic carbon, loss on ignition, dissolved organic carbon, and chemical oxygen demand were reduced by 69.047, 86.922, 85.378, 88.458, and 90.771%, respectively. At the same time, the PDMAX shown was 376.20 ± 15.478 mW/cm2, with a calculated internal resistance of 42.550 ± 12.353 Ω. This technique presents an essential advance in overcoming existing technical barriers because the engineered microbial fuel cells are accessible and scalable. It will generate important value by naturally reducing toxic metals and electrical energy, producing electric currents in a sustainable and affordable way.

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“…One focus of research is the application of MFCs or MECs to search for and evaluate suitable electroactive bacterial strains and consortia [5]. Among others, Geobacter sulfurreducens can be used in MECs as a biocatalyst for hydrogen formation at the cathode [6].…”

Section: Introductionmentioning

confidence: 99%

Current to Biomass: Media Optimization and Strain Selection from Cathode-Associated Microbial Communities in a Two-Chamber Electro-Cultivation Reactor

et al. 2023

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Cathode-associated microbial communities (caMCs) are the functional key elements in the conversion of excess electrical energy into biomass. In this study, we investigated the development of electrochemical caMCs based on two-chamber microbial electrolytic cells (MECs) after optimization of media composition. Microbial communities obtained from a historical soil sample were inoculated into the cathode chamber of MECs. The inorganic medium with (A) carbon dioxide in air or (B) 100 mM sodium bicarbonate as carbon source was used in the absence of any organic carbon source. After 12 days of operation, the experimental results showed that (1) the bacterial community in group B exhibited lush growth and (2) a single strain TX168 Epilithonimonas bovis isolated from group A indicated electrochemical activity and synthesized large volumes of biomass using sodium bicarbonate. We also analyzed the caMCs of the MECs and reference samples without electro-cultivation using 16S rRNA gene sequencing. The results showed that the caMCs of MECs in groups A and B were dominated by the genera Acinetobacter and Pseudomonas. The caMCs were further inoculated and cultured on different agars to isolate specific electroactive bacterial strains. Overall, our study highlights the possibility of converting excess energy into biomass by electro-cultivation and the importance of selecting appropriate media to enrich specific microbial communities and single strains in MECs.

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Iron reducing sludge as a source of electroactive bacteria: assessing iron reduction in biofilm bacteria, planktonic cells and isolates from a microbial fuel cell

Cited by 5 publications

References 29 publications

Exploration of In Vitro Voltage Production by a Consortium of Chemolithotrophic Microorganisms Using Galena (PbS) as a Sulphur Source

Exploration of In Vitro Voltage Production by a Consortium of Chemolithotrophic Microorganisms Using Galena (PbS) as a Sulphur Source

Reduction of Toxic Metal Ions and Production of Bioelectricity through Microbial Fuel Cells Using Bacillus marisflavi as a Biocatalyst

Current to Biomass: Media Optimization and Strain Selection from Cathode-Associated Microbial Communities in a Two-Chamber Electro-Cultivation Reactor

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