Iron potentiates bacterial lipopolysaccharide-induced nitric oxide formation in animal organs

Kubrina, L. N.; Mikoyan, V. D.; Mordvintcev, Peter I.; Vanin, Anatoly F.

doi:10.1016/0167-4889(93)90050-y

Cited by 54 publications

(24 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Fig. 3), which is in agreement with the data reported previously by other investigators using diethyldithiocarbamate (DETC) as a spintrapping reagent in which they detected the presence of the [(MGD)2-Fe2'-NO] complex in various tissues [24]. Among all tissues examined thus far, the liver tissue retained the highest EPR signal intensity of the [(MGD),-Fe"-NO] complex ( Fig.…”

Section: Resultssupporting

confidence: 82%

Spin trapping of nitric oxide produced in vivo in septic‐shock mice

1994

View full text Add to dashboard Cite

A nitric oxide (•NO) spin‐trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy has been employed to measure the in vivo production of •NO in lipopolysaccharide (LPS)‐treated mice. The in vivo spin‐trapping of •NO was performed by injecting into mice a metal—chelator complex, consisting of N‐methyl‐d‐glucamine dithiocarbamate (MGD) and reduced iron (Fe2+), that binds to •NO and forms a stable, water‐soluble [(MGD)2‐Fe2+‐NO] complex, and by monitoring continuously the in vivo formation of the latter complex using an S‐band EPR spectrometer. At 6 h after intravenous injection of LPS, a three‐line EPR spectrum of the [(MGD)2‐Fe2+‐NO] complex, was observed in the blood circulation of the mouse tail; the [(MGD)2‐Fe2+] complex was injected subcutaneously 2 h before EPR measurement. No signal was detected in control groups. Administration of N G‐monomethyl‐l‐arginine, an •NO synthase inhibitor, caused a marked reduction in the in vivo EPR signal of the [(MGD)2‐Fe2+‐NO] complex, suggesting that the •NO detected is synthesized via the arginine‐nitric oxide synthase pathway. The results presented here demonstrated, for the first time, the in vivo real time measurement of •NO in the blood circulation of conscious, LPS‐treated animals.

show abstract

Section: Resultssupporting

confidence: 82%

Spin trapping of nitric oxide produced in vivo in septic‐shock mice

1994

View full text Add to dashboard Cite

show abstract

“…At 77 K, the MNIC-DETC complex showed a characteristic triplet with a N = 12.5 G [33,34], seen in Figure 1. Figure 2 represents the concentrations of ž NO-spin trap adduct measured in all experimental groups 3 weeks after the induction of diabetes.…”

Section: Effect Of Treatments On Nitric Oxide Productionmentioning

confidence: 99%

Beneficial effects of aminoguanidine on the cardiovascular system of diabetic rats

Stadler

Jenei

Somogyi

et al. 2005

Diabetes Metab. Res. Rev.

View full text Add to dashboard Cite

show abstract

“…Some contradictory reports also appeared on the roles of exogenous iron in dithiocarbamates NO scavenging. While iron-citrate administration was found to potentiate bacterial LPS-induced NO production in most organs of treated mice [105], the increase of the NO-Fe 2+ -(DETC) 2 complex EPR signal induced by iron administration was correlated to an enhancement of the DETC spin-trapping ability [106]. These differences in analysis could also be related to uneven distribution of NO synthesis in organs as well as in circulating blood.…”

Section: Possible Artefacts In the Use Of Iron-dithiocarbamate Complementioning

confidence: 99%

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Henry¹,

Guissani²

2000

Analusis

View full text Add to dashboard Cite

In this overview of NO spin-trapping methods, we have pointed out their main advantages over frozen solution EPR spectroscopy detection of nitrosylated metalloproteins: improved sensitivity, potential specificity and time-resolution. A few simple ideas concerning EPR methodology have been recalled. Examples of possible artefacts, sometimes unexpected, which can easily be encountered in any analytical methods have been described. New methodological developments have also been mentioned.

show abstract

Iron potentiates bacterial lipopolysaccharide-induced nitric oxide formation in animal organs

Cited by 54 publications

References 13 publications

Spin trapping of nitric oxide produced in vivo in septic‐shock mice

Spin trapping of nitric oxide produced in vivo in septic‐shock mice

Beneficial effects of aminoguanidine on the cardiovascular system of diabetic rats

Contribution of spin-trapping EPR techniques for the measurement of NO production in biological systems

Contact Info

Product

Resources

About