Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

Borcherding, Jennifer; Baltrušaitis, Jonas; Chen, Haihan; Stebounova, Larissa V.; Wu, Chia‐Ming; Rubasinghege, Gayan; Mudunkotuwa, Imali A.; Caraballo, Juan C.; Zabner, Joseph; Grassian, Vicki H.; Comellas, Alejandro P.

doi:10.1039/c3en00029j

Cited by 97 publications

(75 citation statements)

References 47 publications

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“…A previous report showed that the biofilm mass formed in the presence of TiO 2 NPs appeared to increase in LM2 cultures at all nanoparticles doses utilized in a dose-dependent manner during 24 h incubations with significant values for 80, 8, and 0.8 mg/mL (Ammendolia et al, 2014). It has been shown that the presence of iron, especially FeCl 3 , enhances biofilm formation in P. aeruginosa (Patriquin et al, 2008;Borcherding et al, 2014). Our previous study also reported that EPS production increased with increasing Cr(VI) concentration in the growth medium (Harish et al, 2012).…”

Section: Discussionmentioning

confidence: 90%

Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations

Kumari

Kumar

Mathur

et al. 2014

Environmental Research

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Section: Discussionmentioning

confidence: 90%

Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations

Kumari

Kumar

Mathur

et al. 2014

Environmental Research

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“…the complexity of the interfacial region between the nanoparticle surface and the aqueous phase surrounding it. 24,25 These studies start with a thin film of hydrated nanoparticles placed on to an ATR crystal. 5 Often these media formulations are prepared to simulate and/or be compatible with different types of biological and environmental systems and consist of a variety of inorganic and organic ligands that include, but are not limited to chlorides, phosphates, carbonates, organic acids, natural organic matter (NOMs), steroids, amino acids, proteins and lipids.…”

Section: Surface Transformations Of Nanomaterials In Different Biologmentioning

confidence: 99%

Biological and environmental media control oxide nanoparticle surface composition: the roles of biological components (proteins and amino acids), inorganic oxyanions and humic acid

Mudunkotuwa

Grassian

2015

Environ. Sci.: Nano

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Nano Impact Statement: Surfaces play an important role in the toxicity and fate of nanomaterials. However, what exactly is adsorbed on the surface of nanonomaterials in different biological and environmental media is often unknown. In this study, the surface composition and speciation of oxide nanoparticles -TiO 2 and α α α α-Fe 2 O 3 -in a range of different media. The extent of surface adsorption depends on the solution phase composition and the affinity of different components to adsorb to the nanoparticle surface. Examples presented here show that there are a range of possible surface interactions, adsorption energetics and adsorption modes including reversible adsorption, irreversible adsorption and co-adsorption. AbstractCurrent practices of initial nanoparticle characterization with respect to particle size, shape, surface and bulk composition prior to experiments to test, for example, cellular interaction or toxicity, will not accurately describe nanomaterials in a given medium. The use of initial characterization data in subsequent analyses inherently assumes that nanoparticles are static entities. However, nanoparticle characterization, which is crucial in all studies related to their applications and implications, should also include information about the dynamics of the interfacial region between the nanomaterial surface and the surrounding medium. The objective of this tutorial review is to highlight the importance of in situ characterization of metal oxide nanoparticle surfaces in complex media. In particular, several examples of TiO 2 (5 nm) and α-Fe 2 O 3 (2 nm) nanoparticles, in different environmental and biological media, are presented so as to show the importance of the milieu on oxide surface composition. The surface composition is shown to be controlled by the adsorption of biological components (proteins and amino acids), inorganic oxyanions (phosphates and carbonates) and environmental ligands (humic acid). The extent of surface adsorption depends on the solution phase composition and the affinity of different components to adsorb to the nanoparticle surface. The examples presented here show that there is a range of possible surface interactions, adsorption energetics and adsorption modes including reversible adsorption, irreversible adsorption and co-adsorption.

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“…17 In contrast, it has been reported that iron-containing nanoparticles may influence the ability of bacteria to absorb iron for growth, increase virulence, and inhibit AMP function. 18 Here, we present the intrinsic antifungal activity of the citric acid-coated MnFe 2 O 4 -NPs and the improvement of their antifungal activity when these nanoparticles were conjugated with a 12 aa peptide named Cm-p5. 19 This peptide is derived from Cm-p1, which is an MS-MS sequence found in a tryptic chromatographic fraction from the whole animal extract (Cenchritis muricatus [Gastropoda: Littorinidae], Linnaeus, 1758), which was positive for antimicrobial activity.…”

mentioning

confidence: 99%

“…[55][56][57][58] Indeed, Borcherding et al reported that iron-containing nanoparticles affect the activity of soluble AMPs, probably influenced by the ability of bacteria to scavenge iron for growth from nanoparticles and the adsorption of peptides onto nanoparticle surfaces. 18 It is obvious that AMPs with different physicochemical properties can be affected differently by immobilization reactions. 59 In this sense, Cm-p5 is not an amphipathic peptide, and its antifungal activity does not depend on its amino-terminal group.…”

mentioning

confidence: 99%

The intrinsic antimicrobial activity of citric acid-coated manganese ferrite nanoparticles is enhanced after conjugation with the antifungal peptide Cm-p5

López-Abarrategui¹,

Figueroa-Espí

Lugo-Alvarez³

et al. 2016

IJN

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Diseases caused by bacterial and fungal pathogens are among the major health problems in the world. Newer antimicrobial therapies based on novel molecules urgently need to be developed, and this includes the antimicrobial peptides. In spite of the potential of antimicrobial peptides, very few of them were able to be successfully developed into therapeutics. The major problems they present are molecule stability, toxicity in host cells, and production costs. A novel strategy to overcome these obstacles is conjugation to nanomaterial preparations. The antimicrobial activity of different types of nanoparticles has been previously demonstrated. Specifically, magnetic nanoparticles have been widely studied in biomedicine due to their physicochemical properties. The citric acid-modified manganese ferrite nanoparticles used in this study were characterized by high-resolution transmission electron microscopy, which confirmed the formation of nanocrystals of approximately 5 nm diameter. These nanoparticles were able to inhibit Candida albicans growth in vitro. The minimal inhibitory concentration was 250 µg/mL. However, the nanoparticles were not capable of inhibiting Gram-negative bacteria ( Escherichia coli ) or Gram-positive bacteria ( Staphylococcus aureus ). Finally, an antifungal peptide (Cm-p5) from the sea animal Cenchritis muricatus (Gastropoda: Littorinidae) was conjugated to the modified manganese ferrite nanoparticles. The antifungal activity of the conjugated nanoparticles was higher than their bulk counterparts, showing a minimal inhibitory concentration of 100 µg/mL. This conjugate proved to be nontoxic to a macrophage cell line at concentrations that showed antimicrobial activity.

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Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

Cited by 97 publications

References 47 publications

Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations

Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations

Biological and environmental media control oxide nanoparticle surface composition: the roles of biological components (proteins and amino acids), inorganic oxyanions and humic acid

The intrinsic antimicrobial activity of citric acid-coated manganese ferrite nanoparticles is enhanced after conjugation with the antifungal peptide Cm-p5

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