2002
DOI: 10.1080/00365520212494
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Iron Overload Decreases the Protective Effect of Tumour Necrosis Factor-α on Rat Hepatocytes Exposed to Oxidative Stress

Abstract: TNF-alpha protects rat hepatocytes from normal but not iron-loaded rats from oxidative stress. The protection may be due to an induction of the ferritin synthesis.

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Cited by 8 publications
(9 citation statements)
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“…Cultures were incubated in 3 ml 199 Earles modified salts medium (Gibco BRL, UK) in the presence of 10% FCS (fetal calf serum) for 4 h. The medium was then changed to one containing 5% BSA (bovine serum albumin). Medium was refreshed after 17 and 41 h. Cells were harvested at the time points 0, 1, 3,5,7,9,11,13,15,17,41 and 65 h from the start of incubation with 5% BSA ( Fig. 1(a)).…”
Section: Incubation Of Hepatocytes In 5% Bsa For Different Lengths Ofmentioning
confidence: 99%
See 3 more Smart Citations
“…Cultures were incubated in 3 ml 199 Earles modified salts medium (Gibco BRL, UK) in the presence of 10% FCS (fetal calf serum) for 4 h. The medium was then changed to one containing 5% BSA (bovine serum albumin). Medium was refreshed after 17 and 41 h. Cells were harvested at the time points 0, 1, 3,5,7,9,11,13,15,17,41 and 65 h from the start of incubation with 5% BSA ( Fig. 1(a)).…”
Section: Incubation Of Hepatocytes In 5% Bsa For Different Lengths Ofmentioning
confidence: 99%
“…Freshly isolated hepatocytes were plated and cultured in the 199 Earles modified salts medium containing 10% FCS for 4 h. Then the medium was changed to one containing 5% BSA, and cells were cultured over night (17 h). The cell cultures were then incubated for 48 h in 199 Earles modified salts medium containing 5% BSA with 10 ng/ml of TNF-alpha, 1 mM DFO, 100 mM FAC, or left untreated [13]. Alternatively, the cells were cultured in the same medium containing either 5% BSA or 10% FCS with the addition of TNF-alpha (10 ng/ml), IL-6 (20 ng/ml), LPS (0.1 mg/ml), DFO (1 mM), FAC (100 mM) (all from Sigma, St Louis, MO, USA) for 48 h, or left untreated.…”
Section: Incubation Of Hepatocytes In 5% Bsa and Three Different Seramentioning
confidence: 99%
See 2 more Smart Citations
“…The transient induction of zinc, copper, and iron in the liver after injury may provide an excess pool of intracellular metals for these proteins as well as the formation of free radicals causing liver damage [25]. Also, the transient saturation of these proteins with metals in response to the injury may alter their structure, function, expression, and intracellular location, which might be associated with phenotypic changes in the liver [26,27].…”
Section: Discussionmentioning
confidence: 99%