Iron overload correlates with serum liver fibrotic markers and liver dysfunction: Potential new methods to predict iron overload‐related liver fibrosis in thalassemia patients

Wang, Man; Liu, Rongrong; Liang, Yao; Yang, Gaohui; Huang, Yumei; Yu, Chunlan; Sun, Kaiqi; Lai, Yongrong; Xia, Yang

doi:10.1177/2050640616646525

Cited by 13 publications

(14 citation statements)

References 54 publications

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“…Intensified liver Fe uptake may lead to hepatic Fe overload [50]. It was shown that liver iron overload was associated with elevated blood levels of ALT [51]. However, in our previous studies, we did not find this relationship [47,49].…”

Section: Introductioncontrasting

confidence: 57%

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

et al. 2020

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The influence of probiotic supplementation on iron metabolism remains poorly investigated. However, a range of studies, especially on Lactobacillus plantarum 299v (Lp229v), have indicated a possible positive impact of probiotics on iron absorption. The aim of the study was to determine the effect of multistrain probiotic supply on iron balance. Thirty Wistar rats were randomized into three groups: placebo (KK group), and multistrain probiotic per os in a daily dose of 2.5 × 109 colony forming units (CFU) (PA group) or 1 × 1010 CFU (PB group). Multistrain probiotic consisted of nine bacterial strains: Bifidobacterium bifidum W23, B. lactis W51, B. lactis W52, Lactobacillus acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lactococcus lactis W19, and Lc. lactis W58, in equal proportions. After six weeks, blood and organ samples were collected. No differences were found between the three groups in terms of serum concentrations of hepcidin (HEPC), lactoferrin (LTF), homocysteine (HCY), ferritin (Ft), or erythroferrone (ErFe), or in liver content of divalent metal transporter 1 (DMT1), transferrin receptors 1 and 2 (TfR), or ZRT/IRT-like protein 14 (ZIP14) proteins. In the overall sample, positive correlations were noted between the serum concentrations of hepcidin and lactoferrin, and hepcidin and ferritin; serum concentration of hepcidin and DMT1 and TfR1 in the liver; and serum concentration of erythroferrone and TfR2 in the liver. The correlations of serum hepcidin and erythroferrone with liver DMT1 and TfR represent significant mechanisms of Fe homeostasis. Our study has shown that multistrain probiotic supplementation used in the experiment did not disrupt the biochemical and hepatic regulatory processes of Fe balance and did not demonstrate significant influence on selected parameters of Fe metabolism.

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Section: Introductioncontrasting

confidence: 57%

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

et al. 2020

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“…The ages of the patients and control groups were distributed between 1 year to 44 years. The more significant majority of the infected persons with HCV was in the middle age groups (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) years old, while the lowest rate of infected persons was in the age group (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44) years of old. Gender did not differ significantly among thalassemia patients with or without HCV infection and healthy subjects (Table2).…”

Section: Molecular Rt-pcr Resultsmentioning

confidence: 99%

“…At a global level, between 4.4 % and 85.4 % of the thalassemia patients are positive for anti-HCV antibodies 18 . The distribution of patients according to age were as follow: 73.0% were in the range of (15-29) years; 18.0% in the range (1-14) yearsand 9% inthe range (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44) years. In our study, the mean age of thalassaemic patients with anti-HCV was20±7.0 years.…”

Section: Discussionmentioning

confidence: 99%

“…There are apparent differences in the distribution of thalassemia patients infected with hepatitis C according to the age groups. The highest percent of incidence in the age group (15-29 years) as it was estimated at 73% while the lowest percentage is in the age groups (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44). This may be because early detection of the disease gives more significant opportunity for treatment and cure.…”

Section: Discussionmentioning

confidence: 99%

“…Our patients, especially those with HCV infection, did have a significant depletion of vitamins E and C, indicating that iron overload and HCV infection were more influential than supplementing these nutritional factors in transfusion-dependent thalassemia major patients. HCV infection is an important cause of liver damage especially in the thalassemic patient with multi-blood transfusion; the HCV-related liver damage is characterized by increased iron storage (possibly induced by the virus), which elicits free-radical-mediated peroxidation 37 .A considerable decrease of arachidonic acid and other polyunsaturated fatty acids of the red blood cell membrane, and a decreased level of plasma vitamin E in cirrhotic patients with HCV infection suggest that stimulated polyunsaturated fatty acids oxidation may be associated with the progression of HCV infection in chronic liver disease 38 . At the same time, liver iron storage may be necessary for determining the prognosis of HCV-related chronic hepatitis 39 .In our study, levels of vitamins E and C were significantly lower in the significant thalassemia patients with HCV infection.…”

Section: Discussionmentioning

confidence: 99%

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The Role of Oxidative Stress Markers in Î’-Thalassemic Iraqi Patients Infected With Hepatitis C Virus Diagnosed by Rt-PCR

Ani

Al-Ouqaili²,

Awad

2019

Int J Life Sci Pharm Res

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Blood transfusion is associated with the risk of infection, especially hepatitis C virus and may lead to peroxidative tissue injury by secondary iron overload in β-thalassemic patients. This study aims to detect HCV-RNA copies in thalassemic patients detected by RT-PCR qualitatively and quantitatively. Also, to measure the role of enzymatic and non-enzymatic anti-oxidants markers in β-thalassemia patients infected with HCV. A total of 200 β-thalassemic patients were collected and analyzed for anti-HCV antibodies using Rapid immunochromatographic assay and enzyme-linked Immunosorbent assay methods. Then, the nucleic acids HCV-RNA of positive samples were extracted by modern automated technique. After that, Amplification for HCV-RNA was amplified. The vitamins C and, E in addition to GSH and GGT were also accurately detected. Out of 100 positive ELISA-anti-HCV antibodies, 72(72%) were also positive for RT-PCR considering the assay threshold for the procedure was >13 IU/ ml. The mean viral load in these patients was 545806 ±1009799 IU/ml (1997176 ±3802206) Copies/ml. There was an observed difference (P≤ 0.005) inactivity of TSB, GGT, Protein, Albumin in sera of HCV patients (2.87 ± 1.41, 51.1 ± 60.0 , 6.44 ±2.00 , 3.37 ±0.71) when compared with healthy subjects (0.99 ± 0.46 ; 25.2 ± 12.0 , 7.09 ±0.66 , 3.94 ±0.59) respectively while the Globulin detected evinced no significant difference (P˃ 0.005) in HCV patients (2.90±0.80) as compared with healthy subjects (3.14 ±0.42). Besides there was a significant difference (P≤ 0.005) in serum vitamin C, vitamin E and GSH, between HCV patients groups (57.2 ±46.1, 34.7±14.6, 282.4 ±150.5) and healthy subject groups (73.0 ± 18.0, 44.6 ± 15.6, 353.5± 59.37) respectively. The prevalence of HCV infection was much higher among β-thalassemic patients compared with the healthy blood donors. It is recommended to use nucleic acid based-tests for screening blood donors. The current study shows that there is no correlation between ELISA and viral load in hepatitis C virus infection. On the other hand, Real-Time PCR is a confirmatory diagnostic test and is considered as the golden test for the diagnosis and follow up of hepatitis C virus infection. The HCV infection significantly increases ALT, AST, ALP, GGT, and TSB level above the normal range while vitamins C, E, and GSH decreased considerably

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Effect of procyanidins on lipid metabolism and inflammation in rats exposed to alcohol and iron

Lobo

Liu

Song

et al. 2020

Heliyon

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Background Lifestyle involving uncontrolled alcohol consumption coupled regularly with red meat and other iron sources has detrimental effects on the liver, which in the long term, results in Alcoholic Liver Disease (ALD). Procyanidin has lately garnered increasing attention and has become the focus of research owing to its antioxidant properties. This study explores the anti-inflammatory effects of procyanidins, in preventing ALD, by analyzing the biological activities of the compound on liver injury caused by excessive alcohol and iron. Method Male SPF Wistar rats were placed in 4 groups; the control Group A (basic diet); the model Group B (excess alcohol 8–12 mL/kg/d and iron 1000 mg/kg diet); the low dose procyanidin Group C (model group diet plus 60 mg/kg/d of procyanidin); and the high dose procyanidin Group D (model group diet plus 120 mg/kg/d of procyanidin). Serum biochemical markers for liver damage were measured spectrophotometrically. The NFκB and IκB mRNA expression levels were determined using RT-PCR; the NFκB p65 and IκB protein expression levels were assessed via western blotting, while ELISA was used to detect serum inflammatory factors. Results The pathological score of the model Group B, low and high dose procyanidin Groups C and D were 6.58 ± 0.90,4.69 ± 0.70 and 2.00 ± 0.73, respectively (P < 0.05). The results showed that high alcohol and iron contents in the model group led to significant damage of liver structure, increased low-density lipoproteins (LDLs), steatosis, and increased levels of inflammatory cytokines. High amounts of procyanidins led to the preservation of the liver structure, production of high-density lipoproteins, and reduction in serum inflammatory cytokines while also significantly decreasing the expression levels of NFκB p65. Conclusion The results prove that procyanidins have hepatoprotective potential and could be effective in reversing histopathology, possibly by alleviating inflammation and improving lipid metabolism.

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Iron overload correlates with serum liver fibrotic markers and liver dysfunction: Potential new methods to predict iron overload‐related liver fibrosis in thalassemia patients

Cited by 13 publications

References 54 publications

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

Hepcidin and Erythroferrone Correlate with Hepatic Iron Transporters in Rats Supplemented with Multispecies Probiotics

The Role of Oxidative Stress Markers in Î’-Thalassemic Iraqi Patients Infected With Hepatitis C Virus Diagnosed by Rt-PCR

Effect of procyanidins on lipid metabolism and inflammation in rats exposed to alcohol and iron

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