Iron Metabolism. Heme Synthesis
            <i>in Vitro</i>
            by Immature Erythrocytes

Walsh, R. J.; Thomas, E. Donnall; Chow, Sam; Fluharty, R.G.; Finch, C. A.

doi:10.1126/science.110.2859.396

Cited by 106 publications

(33 citation statements)

References 5 publications

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“…The rise in specific activity of the more resistant (younger) erythrocyte fractions 90 days or more after isotope administration is presumably a result of the reutilization of Fe59. Previous investigators have shown that while intravenously administered Fe59 incorporation is limited to newly forming erythrocytes, isotope released from cells as they are destroyed is available for re-incorporation into immature red cells (8,9,14).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Relationship Between the Age of Human Erythrocytes and Their Osmotic Resistance: A Basis for Separating Young and Old Erythrocytes12

Marks¹,

Johnson²

1958

J. Clin. Invest.

136

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A study has been made of the effect of age on the susceptibility of mature human erythrocytes to hemolysis in hypotonic media. The alterations in osmotic fragility associated with red cell aging are pertinent to the relationship between cellular senescence and the maintenance of cell mambrane integrity. In addition, differences in osmotic resistance between young and old erythrocytes could provide a basis for separating the whole red cell population into fractions of differing mean cell age. A method for achieving such a separation would permit investigation of biochemical changes associated with in sivo aging of erythrocytes.On the basis of experiments with red cells of dogs, Cruz, Hahn, Bale and Balfour (2) and Stewart, Stewart, Izzo and Young (3) concluded that young canine erythrocytes were less resistant to hypotonic lysis than older cells. Recent evidence suggests that the young erythrocytes of rabbit (4, 5), rat and (6) and man (7) are more resistant to hemolysis in hypotonic media than are older cells.In the present investigation, Fe59 has been employed to label, in vivo, erythrocytes of a limited age (8-11). It has been found that graded osmotic hemolysis may achieve a separation of red cells into fractions of relatively young and old mean cell ages. The young human erythrocytes are more resistant to hemolysis in hypotonic media than older cells. METHODSThe subjects studied included four persons in good health (Subjects LO, LA, DE and LI) and four patients with neoplastic disease. Among these patients, one had carcinoma of the breast (Subject W), two had car-

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Section: Resultsmentioning

confidence: 99%

“…In the present investigation, Fe59 has been employed to label, in vivo, erythrocytes of a limited age (8)(9)(10)(11). It has been found that graded osmotic hemolysis may achieve a separation of red cells into fractions of relatively young and old mean cell ages.…”

mentioning

confidence: 99%

Relationship Between the Age of Human Erythrocytes and Their Osmotic Resistance: A Basis for Separating Young and Old Erythrocytes12

Marks¹,

Johnson²

1958

J. Clin. Invest.

136

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“…Walsh, Thomas, Chow, Fluharty, and Finch (37) have observed that human reticulocytes can incorporate Fe' into hemoglobin in vitro. Accordingly, blood samples were utilized from several patients with reticulocyte-rich blood for observations of Fe"-labelled reticulocyte survival in vivo.…”

Section: Methodsmentioning

confidence: 99%

The Destruction of Red Cells by Antibodies in Man. I. Observations on the Sequestration and Lysis of Red Cells Altered by Immune Mechanisms1

Jandl¹,

Jones²,

Castle³

1957

J. Clin. Invest.

229

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“…At the surface of the red cell precursor it is released from transferrin onto the cell membrane and is incorporated rapidly into hemoglobin (1)(2)(3)(4). The mechanism of the movement of iron from the cell membrane to the site of hemoglobin synthesis is not understood.…”

Section: Introductionmentioning

confidence: 99%

Studies on the partition of iron in bone marrow cells

Primosigh¹,

Thomas²

1968

J. Clin. Invest.

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A B S T R A C T Canine marrow cells were incubated with transferrin-bound 59Fe, and the partition of cellular iron was studied by chromatographic and gel filtration methods. Splitting-off of iron from the stromal fraction was avoided by lysing the cells in Tris HCl buffer at pH 8.6. Cellular iron was divided into four major compartments: stroma, microsomes, main hemoglobin, and fraction I. The iron in fraction I was found in ferritin, heme proteins, and low molecular weight iron.With incubation times of 3-10 min, 59Fe appeared promptly in the main hemoglobin. The entry of 59Fe into ferritin paralleled that of hemoglobin but was smaller in amount. When the marrow cells were incubated with 59Fe for 15-20 min and reincubated without radioactive iron, movement of 59Fe into main hemoglobin was observed, and essentially all this iron came from the particulate fraction (stroma, mitochondria, and microsomes). In these chase experiments there was no change in the total quantity of 59Fe in ferritin. There was no evidence of a significant hemoglobin precursor other than low molecular weight iron.Depending upon concentration, lead was observed to inhibit cellular iron metabolism at several points: uptake of iron by the cell, movement of iron from stroma to the soluble intracellular compartment, and synthesis of hemoglobin. The most pronounced inhibitory effect of lead was always on hemoglobin synthesis with an increase in ferritin: hemoglobin ratio. Bipyridine appeared to trap intracellular ferrous iron and to inhibit synthesis of both hemoglobin and ferritin.

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Iron Metabolism. Heme Synthesis in Vitro by Immature Erythrocytes

Cited by 106 publications

References 5 publications

Relationship Between the Age of Human Erythrocytes and Their Osmotic Resistance: A Basis for Separating Young and Old Erythrocytes12

Relationship Between the Age of Human Erythrocytes and Their Osmotic Resistance: A Basis for Separating Young and Old Erythrocytes12

The Destruction of Red Cells by Antibodies in Man. I. Observations on the Sequestration and Lysis of Red Cells Altered by Immune Mechanisms1

Studies on the partition of iron in bone marrow cells

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