Iron metabolism genes in Antarctic notothenioids: A review

Scudiero, Rosaria; Trinchella, Francesca; Parisi, Elio

doi:10.1016/j.margen.2008.12.001

Cited by 6 publications

(9 citation statements)

References 45 publications

(52 reference statements)

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“…1A and 2), are generally found in white-blooded notothenioids compared with red-blooded species. From the results presented herein, it is clear that similarities (e.g., Tf) and differences (e.g., Ft) in concentrations of Fe-binding proteins among white-and red-blooded species are in general agreement with previous reports describing relative expression of mRNA for Fe-binding proteins (51,52).…”

Section: Discussionsupporting

confidence: 91%

“…We suggest that similarities in total contents of plasma non-heme Fe and of Fe bound to Tf reflect Fe uptake from the diet that may, in fact, be comparable among notothenioid species. Similar rates of Fe acquisition in white-and redblooded notothenioids is also supported by a comparison of Asc levels (this study) and divalent metal transporter (DMT) in notothenioids (51,52). Asc can reduce ferric iron to ferrous iron, and although this is not the sole mechanism by which Fe is reduced (35,67), Asc has been shown to increase significantly the uptake of Fe in a marine teleost (11).…”

Section: Discussionsupporting

confidence: 68%

“…To date, DMT1 in the intestine of Antarctic fishes has yet to be quantified; however, mRNA levels of DMT1 in the majority of tissues examined (kidney, liver, and spleen) are not significantly different among white-and red-blooded notothenioids (51,52). The exception is found in the hematopoietic head kidney, which in red-blooded T. bernacchii contains elevated levels of DMT1 mRNA compared with the head kidney from the icefish C. rastrospinosus (51,52).…”

Section: Discussionmentioning

confidence: 97%

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Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes

Kuhn

O’Brien

Crockett

2016

American Journal of Physiology-Regulatory, Integrative and Comp

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The family Channichthyidae or “icefishes” (suborder Notothenioidei) represents the only vertebrates lacking hemoglobin (Hb) as adults. Several icefish species also do not express cardiac myoglobin (Mb). We address how levels of proteins involved in iron (Fe) processing (transport, sequestration, and export) vary among white- and red-blooded notothenioids, and whether absence of Hb and/or Mb in channichthyids is accompanied by expansion of contents of Fe-binding proteins to protect against unchaperoned Fe. Levels of transferrin (Tf), ferritin (Ft), ceruloplasmin (Cp), and non-heme Fe were quantified in plasma, serum, and/or nonhematopoietic tissues (cardiac ventricle, skeletal muscle, and liver) from species of white-blooded ( Chaenocephalus aceratus, Champsocephalus gunnari, Chionodraco rastrospinosus, Pseudochaenichthys georgianus) (the first two species not expressing Mb) and red-blooded ( Notothenia coriiceps, Gobionotothen gibberifrons) notothenioids. We also measured levels of ascorbate (Asc), a mediator of Fe uptake. While plasma concentrations of Tf and tissue levels of Asc are similar among species, concentrations of plasma Asc are lower in white-blooded species. Concentrations of Ft and non-heme Fe and activities of Cp are also generally reduced in icefishes compared with red-blooded notothenioids. The presence of cardiac Mb in some icefish species does not appear to influence levels of proteins involved in Fe processing. To address further the question of Fe sequestration within a physiological context, we account for well-characterized differences in blood volume and heart mass among white- and red-blooded notothenioids. We report that total contents of plasma Tf are greater, while ventricle non-heme Fe is at least at parity in white- vs. red-blooded species.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes

Kuhn

O’Brien

Crockett

2016

American Journal of Physiology-Regulatory, Integrative and Comp

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“…(Buonocore et al, 2016). Furthermore, cDNAs of iron metabolism proteins have been cloned, e.g., ceruloplasmin (Cp), transferrin (Tf ), ferritin (Fth1) and divalent metal transporter 1 (Dmt1) (Scudiero et al, 2007(Scudiero et al, , 2009, but no specific information is available on how they are regulated before an infectious process. Furthermore, there is no information on nutritional immunity associated with iron in Antarctic fish or if this type of immune response could be activated by the detection of pathogen-associated molecular patterns (PAMPs), which activate the immune system, but do not require iron.…”

Section: Introductionmentioning

confidence: 99%

LPS Modulates the Expression of Iron-Related Immune Genes in Two Antarctic Notothenoids

et al. 2020

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The non-specific immunity can induce iron deprivation as a defense mechanism against potential bacterial pathogens, but little information is available as to its role in Antarctic fish. In this study the response of iron metabolism related genes was evaluated in liver and head kidney of the Antarctic notothenoids Notothenia coriiceps and Notothenia rossii 7 days after lipopolysaccharide (LPS) injection. Average plasma Fe 2+ concentration was unaffected by treatment in any of the species. The gene expression response to LPS varied between tissues and species, being stronger in N. coriiceps and more prominent in the head kidney than liver. The reaction to LPS was marked by increased individual variability in most genes analyzed, even when the change in expression was not statistically significant, suggesting different individual sensitivity and coping responses in these wild fish. We found that iron related genes had an attenuated and homogenous response to LPS but there was no detectable relationship between plasma Fe 2+ and gene expression. However, overall in both tissues and species LPS exposure set a multilevel response that concur to promote intracellular accumulation of iron, an indication that Antarctic Notothenoids use innate nutritional immunity as a resistance mechanism against pathogens.

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“…Recently, many nucleotidic sequences of H-chain ferritins were characterized from Antarctic teleosts [19][20][21]; on the contrary, sequences of the M-chain ferritins from Antarctic teleosts were only from the direct amino acid sequencing of the native proteins [15,22]. No L subunits were found in Antarctic fish.…”

Section: Introductionmentioning

confidence: 99%

Middle ferritin genes from the icefish Chionodraco rastrospinosus: Comparative analysis and evolution of fish ferritins

Scudiero

Esposito

Trinchella

2013

Comptes Rendus. Biologies

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Iron metabolism genes in Antarctic notothenioids: A review

Cited by 6 publications

References 45 publications

Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes

Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes

LPS Modulates the Expression of Iron-Related Immune Genes in Two Antarctic Notothenoids

Middle ferritin genes from the icefish Chionodraco rastrospinosus: Comparative analysis and evolution of fish ferritins

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