Iron(III) oxidized nucleophilic coupling of catechol with o-tolidine/p-toluidine followed by 1,10-phenanthroline as new and sensitivity improved spectrophotometric methods for iron present in chemicals, pharmaceutical, edible green leaves, nuts and lake water samples

Ex Vivo and in Vivo Study of Sucrosomial® Iron Intestinal Absorption and Bioavailability

“…Then, at 30 min and 1, 2, 3, 4 and 5 h after gavage (about 400 μL corresponding to 5 mg/kg of iron-containing SRM, FITC-labeled SRM or FP), samples were analyzed for Fe 3+ or fluorescence. Each blood sample was immediately centrifuged (2040× g , 20 min, 4 °C) and the serum separated (150 μL) and analyzed for Fe 3+ , as previously described [ 22 ].…”

“…The area under the curve (AUC) of Fe 3+ or FITC-labeled SRM concentration in the blood versus time and over the level of 238 µg/dL, which corresponds to the mean basal Fe 3+ concentration found in rats at time 0 before administration of the formulations under study, was calculated by the linear trapezoidal rule between time 0 and 5 h. After completion of the bioavailability study, animals were sacrificed and liver, spleen and bone marrow were withdrawn and homogenized for 10 min at 8000 rpm with a PBS made to pH 3 [ 23 ]. The homogenate was centrifuged for 5 min at 10,000 rpm and the supernatant analyzed for Fe 3+ content [ 22 ].…”

Hydrothermal synthesis of nitrogen-doped carbon quantum dots from microcrystalline cellulose for the detection of Fe3+ ions in an acidic environment

“…In order to evaluate the accuracy of this uorescence quenching method, a standard method of phenanthroline spectrophotometry was also carried out for ref. 52. Fig.…”

Sucrosomial® iron absorption studied by in vitro and ex-vivo models

“…The study of Fe 3+ release in simulated intestinal fluid was not attempted, as the ferric ion is insoluble at the relevant pH. The withdrawn samples of receiving phase were analyzed spectrophotometrically for Fe 3+ (Shyla et al, 2012) after centrifugation at 14,000 rpm for 10 min. Blank runs showed the absence of significant interference with the spectrophotometric measurements.…”