Iron deficiency specific cDNA (<i>Ids1</i>) with two homologous cysteine rich mt domains from the roots of barley

Okumura, Nami; Nishizawa, Naoyuki; Umehara, Yosuke; Ohata, Tomoko; Mori, Satoshi

doi:10.1080/01904169209364465

Cited by 17 publications

(15 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ehimehadaka No. 1) were germinated and transplanted to the water-culture as described previously [ 33 ]. Fe was supplied (0.5 mg/1) by a 1 ~o iron citrate stock solution when required.…”

Section: Plant and Culture Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare

et al. 1994

Self Cite

View full text Add to dashboard Cite

A lambda zapII cDNA library was constructed from mRNA isolated from Fe-deficient barley roots and screened with cDNA probes made from mRNA of Fe-deficient and Fe-sufficient (control) barley roots. Seven clones were selected. Among them a clone having the putative full-length mRNA of dioxygenase as judged by northern hybridization was selected and named Ids2 (iron deficiency-specific clone 2). Using a cDNA fragment as probe, two clones from the genomic library (lambda EMBL-III) were isolated and one was sequenced. The predicted amino acid sequence of Ids2 resembled that of 2-oxoglutarate-dependent dioxygenase. Ids2 is expressed in the Fe-deficient barley roots but is not in the leaves. The expression is repressed by the availability of Fe. Ids2 was also strongly expressed under Mn deficiency and weakly under Zn deficiency or excess NaCl (0.5%). The upstream 5'-flanking region of Ids2 has a root-specific cis element of the CaMV 35S promoter and a nodule-specific element of leghemoglobin, a metal regulatory element (MRE) and several Cu regulatory elements (UAS) of yeast metallothionein (CUP1).

show abstract

“…Ehimehadaka No. 1) were germinated and transplanted to the water-culture as described previously [ 33 ]. Fe was supplied (0.5 mg/1) by a 1 ~o iron citrate stock solution when required.…”

Section: Plant and Culture Conditionsmentioning

confidence: 99%

“…1. which encodes class-1 MT [32,33] that is homologous to a number of genes which have recently been isolated from other plant species [3,4,6,15,43]. This paper reports the genomic sequence oflds2 which is also specifically expressed in the roots of Fe-deficient barley.…”

Section: Introductionmentioning

confidence: 95%

A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare

et al. 1994

Self Cite

View full text Add to dashboard Cite

show abstract

“…Animal metallothionein is thought to also act as a reservoir for Zn 2+. Its expression increases in response to elevated [Zn 2 + ], and transcription factors which associate with, and activate expression from, the promoters of animal metallothionein genes in response to Zn 2 + have been identified [1,33,34]. Animal metallothionein also shows programmed changes in expression at certain stages of cellular development.…”

Section: Introductionmentioning

confidence: 99%

Expression of the type 2 metallothionein-like gene MT2 from Arabidopsis thaliana in Zn2+-metallothionein-deficient Synechococcus PCC 7942: putative role for MT2 in Zn2+ metabolism

1996

View full text Add to dashboard Cite

Zn2+ proteins pervade metabolism and are essential for gene expression. However, no proteins have been ascribed the central roles of Zn2+ donation to, or removal from, metalloproteins, or Zn2+ storage in vegetative plant tissue. In animals, such functions have been proposed for metallothioneins. Plants contain multiple metallothionein-like genes but their predicted products, which differ significantly from animal metallothioneins, remain to be isolated from vegetative tissue and their roles are uncertain. The type 2 metallothionein-like gene from Arabidopsis, MT2, was expressed under the control of Zn2+-responsive elements derived from the cyanobacterial metallothionein divergon, smt. Zn2+-dependent expression of MT2 transcripts in Synechococcus PCC 7942 was confirmed by northern analysis. The Arabidopsis MT2 gene partly complemented Zn2+ hypersensitivity in mutants of Synechococcus PCC 7942 which are functionally deficient in an endogenous Zn2+-metallothionein gene, smtA. MT2 was also expressed as a recombinant fusion protein in Escherichia coli, purified and shown to bind Zn2+ in vitro. The mean pH of half displacement of Zn2+ from MT2 was estimated to be 5.05. This suggests that MT2 has a greater affinity for Zn2+ than phytochelatins. The results presented here reveal that MT2 is capable of binding Zn2+ in vitro, conferring tolerance to elevated [Zn2+] in vivo within cyanobacteria and is likely to compete with other polypeptides for cellular Zn2+ in planta.

show abstract

“…Comparison of 2D profiles of proteins in barley (Hordeum vulgare L.) roots under Fesufficient and Fe-deficient conditions (Suzuki et al, 1995(Suzuki et al, , 1997 allowed us to identify a 36-kD protein that was specifically induced by Fe deficiency. In addition, several genes related to the Fe-deficiency response have been reported: Ids1 (Okumura et al, 1991), Ids2 (Okumura et al, 1994), and Ids3 (Nakanishi et al, 1993). In this study, we characterized several other proteins induced by Fedeficiency stress in barley roots, one of which was identified as FDH.…”

mentioning

confidence: 99%

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

et al. 1998

Self Cite

View full text Add to dashboard Cite

To identify the proteins induced by Fe deficiency, we have compared the proteins of Fe-sufficient and Fe-deficient barley (Hordeum vulgare L.) roots by two-dimensional polyacrylamide gel electrophoresis. Peptide sequence analysis of induced proteins revealed that formate dehydrogenase (FDH), adenine phosphoribosyltransferase, and the Ids3 gene product (for Fe deficiency-specific) increased in Fe-deficient roots. FDH enzyme activity was detected in Fe-deficient roots but not in Fe-sufficient roots. A cDNA encoding FDH (Fdh) was cloned and sequenced. Fdh expression was induced by Fe deficiency. Fdh was also expressed under anaerobic stress and its expression was more rapid than that induced by Fe deficiency. Thus, the expression of Fdh observed in Fe-deficient barley roots appeared to be a secondary effect caused by oxygen deficiency in Fe-deficient plants.In Fe-deficient calcareous soils graminaceous plants secrete mugineic acid family phytosiderophores, which are natural Fe chelators, from the roots (Takagi, 1976) to solubilize Fe required for plant growth. This Fe-acquisition mechanism in graminaceous plants is called strategy II and in nongraminaceous plants it is called strategy I (Takagi et al., 1984;Marschner et al., 1986). The pathway of the biosynthesis of mugineic acid family phytosiderophores has been established Nishizawa, 1987, 1989;Shojima et al., 1989Shojima et al., , 1990Mori et al., 1990;Ma and Nomoto, 1993). Among the enzymes involved in this biosynthetic pathway, Higuchi et al. (1994Higuchi et al. ( , 1996 purified nicotianamine synthase and Kanazawa et al. (1994) purified nicotianamine aminotransferase. Comparison of 2D profiles of proteins in barley (Hordeum vulgare L.) roots under Fesufficient and Fe-deficient conditions (Suzuki et al., 1995(Suzuki et al., , 1997 allowed us to identify a 36-kD protein that was specifically induced by Fe deficiency. In addition, several genes related to the Fe-deficiency response have been reported: Ids1 (Okumura et al., 1991), Ids2 (Okumura et al., 1994), and Ids3 (Nakanishi et al., 1993. In this study, we characterized several other proteins induced by Fedeficiency stress in barley roots, one of which was identified as FDH. FDH was induced not only by Fe deficiency but also by anaerobic stress. The relationship between Fe deficiency and anaerobic stress in barley roots is discussed. MATERIALS AND METHODS Plant Material and Growth ConditionsSeeds of barley (Hordeum vulgare L. cv Ehimehadaka no. 1) were germinated at room temperature on paper towels soaked with distilled water. Plants were transferred 4 d after germination to a plastic net floating on tap water at pH 5.5 in a greenhouse under natural light. On d 10, plants were transferred to a continuously aerated nutrient solution of the following composition: 0.7 mm K 2 SO 4 , 0.1 mm KCl, 0.1 mm KH 2 PO 4 , 2.0 mm Ca(NO 3 ) 2 , 0.5 mm MgSO 4 , 10 m H 3 BO 3 , 0.5 m MnSO 4 , 0.2 m CuSO 4 , 0.5 m ZnSO 4 , 0.01 m (NH 4 ) 6 Mo 7 O 24 , and 0.1 mm Fe-EDTA. The pH of the culture solution was adjusted to 5.5 da...

show abstract

Iron deficiency specific cDNA (Ids1) with two homologous cysteine rich mt domains from the roots of barley

Cited by 17 publications

References 13 publications

A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare

A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare

Expression of the type 2 metallothionein-like gene MT2 from Arabidopsis thaliana in Zn2+-metallothionein-deficient Synechococcus PCC 7942: putative role for MT2 in Zn2+ metabolism

Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots1

Contact Info

Product

Resources

About