Iron deficiency in pea plants effect on catalase, peroxidase, chlorophyll and proteins of leaves

Rı́o, Luis A. del; Gómez, M. A.; Yáñez, J.; Leal, A.; Gorgé, J. López

doi:10.1007/bf02149743

Cited by 30 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lincoln) were grown in the greenhouse in aerated full nutrient solutions under optimum conditions as described previously (9). The Mn SOD was purified to homogeneity from pea leaves by the procedure of Sevilla et al (40).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Manganese Superoxide Dismutase from the Higher Plant Pisum sativum

Sevilla¹,

López-Gorgé²,

Rı́o³

1982

Plant Physiol.

View full text Add to dashboard Cite

A manganese-containin superoxide dismutase (EC 1.15.1.1) was fully characterized from leaves of the higher plant Pisum sativum L., var. Lincoln. The amino acid composition determined for the enzyme was compared with that of a wide spectrum of superoxide dismutases and found to have a highest degree of homology with the mitochondrial manganese superoxide dismutases from rat liver and yeast. The enzyme showed an apparent pH optimum of 8.6 and at 25°C had a maximum stability at alkaline pH values. By kinetic competition experiments, the rate constant for the disproportionation of superoxide radicals by pea leaf manganese superoxide dismutase was found to be 1.61 x 10' molar1-.second-' at pH 7.8 and 25°C. The enzyme was not sensitive to NaCN or to H202, but was inhibited by N,-. The sulfhydryl reagent p-hydroxymercuribenzoate at 1 mm concentration produced a nearly complete inhibition of the manganese superoxide dismutase activity. The metal chelators o.phenanthrollne, EDTA, and diethyldithiocarbamate all inhibited activity slightly in decreasing order of intensity. A comparative study between this higher plant manganese superoxide dismutase and other dismutases from different origins is presented.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of a Manganese Superoxide Dismutase from the Higher Plant Pisum sativum

Sevilla¹,

López-Gorgé²,

Rı́o³

1982

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Tolerance differences between scion/rootstock combinations may be related to differences in ROS production and/or antioxidant system e ciency. The enzymatic antioxidant system response differs according to plant species and the de cient minerals [56][57][58][59][60] . Enzymatic antioxidant systems in common clementine leaves differed depending on the rootstock genotypes ( Fig.…”

Section: Effect Of Rootstock Ploidy Level On Anatomical Properties Ofmentioning

confidence: 99%

Tetraploid Citrumelo 4475 (Citrus paradisi L. Macf. × Poncirus trifoliata L. Raf.) Rootstocks Improve Common Clementine Tolerance to Long-term Nutrient Deficiency (Citrus clementina Hort. ex Tan)

Oustric

Herbette²,

Quilichini

et al. 2020

Preprint

View full text Add to dashboard Cite

Nutrient deficiency alters growth and the production of high-quality nutritious food. In Citrus crops, rootstock technologies have become a key tool for enhancing tolerance to abiotic stress. The use of doubled diploid rootstocks can improve adaptation to lower nutrient inputs. This study investigated leaf structure and ultrastructure and physiological and biochemical parameters of common clementine scions (C) grafted on diploid (2x) and doubled diploid (4x) Carrizo citrange (C/CC2x and C/CC4x) and Citrumelo 4475 (C/CM2x and C/CM4x) rootstocks under optimal fertigation and after seven months of nutrient deficiency. Rootstock ploidy level had no impact on structure but induced changes in the number and/or size of cells and some cell components of common clementine leaves under optimal nutrition. Rootstock ploidy level did not modify gas exchanges in Carrizo citrange but induced a reduction in the leaf net photosynthetic rate in Citrumelo 4475. By assessing foliar damage, changes in photosynthetic processes and malondialdehyde accumulation, we found that C/CM4x were less affected by nutrient deficiency than the other scion/rootstock combinations. Their greater tolerance to nutrient deficiency was probably due to the better performance of the enzyme-based antioxidant system. Nutrient deficiency had similar impacts on C/CC2x and C/CC4x. Tolerance to nutrient deficiency can therefore be improved by rootstock polyploidy but remains dependent on the rootstock genotype.

show abstract

“…These effects are limited to the chloroplasts, affecting even their "ultrastructure" (Pushnik and Miller, 1982), while other cell organdíes such as mitochondria and peroxisomes seem unaffected (Platt-Aloia et al, 1982). Iron deficiency has been investigated in different plant materials such as maize (Stocking, 1975 andAlmela et al, 1983), pea (Del Rio et al, 1978 andAbadia et al, 1989), barley (Pushnik and Miller, 1982), sugar beet (Terry, 1980 andPlatt-Aloia et al, 1983) apricot and pear , and citrus (Alcaraz et al, 1986).…”

Section: Introductionmentioning

confidence: 98%

Iron deficiency in citrus limon: effects on photo‐chlorophyllase synthetic pigments and chlorophyllase activity

Fernández-López

Almela

López-Roca

et al. 1991

Journal of Plant Nutrition

View full text Add to dashboard Cite

The photosynthetic pigment composition of chlorotic leaves of Citrus limon L. cv. Verna, grown in the field under iron deficiency conditions was determined. A Fe-polyflavonoid was used as fertilizer to control iron chloro-sis. The photosynthetic pigment content and the chlorophyllase activity were determined at 20 day intervals during the deficiency recovery period and compared to untreated similar material. The corresponding differences among treated and untreated control material were analyzed. Iron application increased the levels of all pigments, but the extent of the increase depended on the pigment affected. The chlorophylls/carotenoids and ß-carotene/xantophylls ratios were increased as chlorosis diminished. A multivariance analysis was performed with the data obtained which revealed that chlorophyll a and ß-carotene had the highest correlation coefficient. The chlorophyllase activity did not show significant changes, but it was lower in the treated leaves than in the untreated control leaves during all the sampling cycle.

show abstract

Iron deficiency in pea plants effect on catalase, peroxidase, chlorophyll and proteins of leaves

Cited by 30 publications

References 15 publications

Characterization of a Manganese Superoxide Dismutase from the Higher Plant Pisum sativum

Characterization of a Manganese Superoxide Dismutase from the Higher Plant Pisum sativum

Tetraploid Citrumelo 4475 (Citrus paradisi L. Macf. × Poncirus trifoliata L. Raf.) Rootstocks Improve Common Clementine Tolerance to Long-term Nutrient Deficiency (Citrus clementina Hort. ex Tan)

Iron deficiency in citrus limon: effects on photo‐chlorophyllase synthetic pigments and chlorophyllase activity

Contact Info

Product

Resources

About