Iron-deficiency anemia is associated with high concentrations of transferrin receptor in serum

Punnonen, Kari; Irjala, Kerttu; Rajamäki, Allan

doi:10.1093/clinchem/40.5.774

Cited by 129 publications

(53 citation statements)

References 13 publications

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“…Serum levels of soluble transferrin receptor (sTfR) have been extensively reported to be elevated in iron deficiency (Flowers et al 1989;Kohgo et al 1989;Huebers et al 1990;Punnonen, Irjala & Rajamaki 1994) and to be unaffected by chronic disease (Punnonen et al 1994;Kohgo et al 1988;Ferguson et al 1992). The rationale is that transferrin receptors, which mediate cellular uptake of iron, are expressed in proportion to the cell requirement for iron, and that cell-associated transferrin receptor is subject to proteolysis that generates a soluble form of the receptor (sTfR), which circulates in plasma.…”

Section: Introductionmentioning

confidence: 99%

Serum soluble transferrin receptor and the prediction of marrow aspirate iron results in a heterogeneous group of patients

Means

Allen

Sears

et al. 1999

Clinical &Amp; Laboratory Haematology

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Serum soluble transferrin receptor (sTfR) concentration has been evaluated in the diagnosis of iron deficiency in otherwise healthy individuals and in patients with rheumatoid arthritis, but has not been studied in a general population of patients with complicated clinical presentations. In this study, 145 anaemic patients with a variety of medical conditions undergoing diagnostic bone marrow aspiration for any reason were tested by a complete blood count, a panel of biochemical tests to evaluate iron status, bone-marrow aspirate iron stain, and serum sTfR concentration. Sixteen per cent lacked stainable iron in the marrow aspirate. All biochemical parameters differed significantly between patients with or without stainable marrow iron. The sTfR assay was significantly more sensitive but less specific than other iron status assays in identifying the absence of stainable iron. Logistic regression analysis demonstrated that only sTfR and ferritin contributed independently to the prediction of marrow iron status. Serum ferritin alone was highly specific but insensitive. A decision algorithm combining serum ferritin and sTfR was as sensitive as TfR and as specific as serum ferritin. The measurement of serum sTfR, especially in conjunction with serum ferritin, is a valuable addition to the existing methods for predicting the results of marrow aspirate iron stains.

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Section: Introductionmentioning

confidence: 99%

Serum soluble transferrin receptor and the prediction of marrow aspirate iron results in a heterogeneous group of patients

Means

Allen

Sears

et al. 1999

Clinical &Amp; Laboratory Haematology

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“…Consequently, serum TfR measurement was introduced as a useful indicator of bone marrow erythropoiesis (Kohgo et al, 1987;Muta et al, 1987;Shintani et al, 1994). Recent studies have shown that the measurement of serum TfR is a dependable test for the diagnosis of iron deficiency and is useful for differentiating IDA from the anaemia of chronic disease and inflammation (Skikne et al, 1990;Petterson et al, 1994;Punnonen et al, 1994Punnonen et al, , 1997Gimferrer et al, 1997;North et al, 1997). It may be speculated that, similarly to serum TfR, the number of cells carrying TfR would also be ᭧ 1999 Blackwell Science Ltd Correspondence: Professor Ç igdem Altay, Department of Paediatrics, Ihsan Dogramacı Children's Hospital, Hacettepe University, 06100 Ankara, Turkey.…”

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confidence: 99%

Transferrin receptor on peripheral blood lymphocytes in iron deficiency anaemia

Altay

1999

Br J Haematol

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Summary. The effect of iron deficiency anaemia (IDA) on CD71 expression by peripheral blood lymphocytes was studied in 43 children with iron deficiency anaemia. 18 healthy age-matched children were selected as the control group. 11 children with b-thalassaemia trait were also studied. Lymphocytes bearing CD71 were enumerated by flow cytometric analysis of peripheral blood. At diagnosis, CD71 þ peripheral lymphocytes (mean Ϯ SE) was 5·90 Ϯ 0·76% in patients with IDA and 12·60 Ϯ 0·98% in healthy controls (P ¼ 0·000). In b-thalassaemia trait patients the peripheral blood CD71 þ lymphocytes were 7·80 Ϯ 1·20%. In IDA patients there was a statistically significant correlation between the levels of CD71 þ peripheral lymphocytes and haemoglobin value (P ¼ 0·000). In 19 patients studied at days 0 and 30 of oral iron therapy, the number of peripheral blood CD71 þ lymphocytes was shown to be increased from 5·90 Ϯ 0·76% to 12·11 Ϯ 1·21%. In severe IDA presence of a limited number of CD71 þ peripheral blood lymphocytes indicated that severe IDA should be borne in mind when considering conditions responsible for the suppression of lymphocyte proliferation.

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“…Elevated levels of sTfR were found in the serum of patients with sickle cell anemia (Flowers et al, 1989), b-thalassemia with hemoglobin E or H disease (Huebers & Finch, 1987), hereditary spherocytosis and autoimmune hemolytic anemia (Huebers et al, 1990), but sTfR is mainly used for the differentiation of iron deficiency anemia, accompanied by an increase in the sTfR level, from anemia of chronic disease, which has normal sTfR levels (Ferguson et al, 1992;Punnonen, Irjala & Rajamaki, 1994). sTfR levels can increase threefold to sevenfold in iron deficiency anemia and up to eightfold in b-thalassemia/Hb E disease (Thorstensen & Romslo, 1993).…”

Section: S U M M a R Ymentioning

confidence: 99%

Comparison of soluble and placental transferrin receptors as standards for the determination of soluble transferrin receptor in humans

Коган

Филатов

Kara

et al. 2007

Int J Lab Hematology

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Transferrin receptor is a transmembrane protein that mediates iron transport from blood into cells. The extracellular part of this receptor circulates in blood as soluble transferrin receptor (sTfR) and the immunological determination of this parameter is widely used in clinical practice. This study aimed at comparing the properties of sTfR and placental TfR (pTfR) and to evaluate the validity of pTfR as a standard for the determination of sTfR in human serum. sTfR and pTfR were studied by immunofluorescent assay and fast protein liquid chromatography (FPLC) gel filtration. Serum sTfR levels were calculated using sTfR or pTfR as a standard. The immunological activity of pTfR was lower than that of sTfR in all anti-TfR monoclonal antibody pairs. Upon FPLC gel filtration, pTfR eluted in a void volume of the column as a protein with a molecular weight (MW) of >1500 kDa, whereas the MW of sTfR corresponded to 237 kDa. This could be a result of micelle formation by pTfR because of its hydrophobic intracellular part. The serum sTfR levels calculated against sTfR were 2.5 times lower than those calculated against pTfR. Serum sTfR levels are overestimated when pTfR is used as the standard.

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Iron-deficiency anemia is associated with high concentrations of transferrin receptor in serum

Cited by 129 publications

References 13 publications

Serum soluble transferrin receptor and the prediction of marrow aspirate iron results in a heterogeneous group of patients

Serum soluble transferrin receptor and the prediction of marrow aspirate iron results in a heterogeneous group of patients

Transferrin receptor on peripheral blood lymphocytes in iron deficiency anaemia

Comparison of soluble and placental transferrin receptors as standards for the determination of soluble transferrin receptor in humans

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