Iron-based mimetic enzyme sensor for NO photorelease from sodium nitroprusside

Huang, Peter H.; Zhang, Birong; Dang, Xueping; Chen, Huaixia; Zheng, Dongyun

doi:10.1016/j.jelechem.2020.113890

Cited by 8 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sodium nitroprusside (Na 2 [Fe(CN) 5 NO]), a widely used exogenous NO donor 34 , 35 , was dissolved in distilled water as a 10 mM stock solution immediately before the experiments to avoid direct light exposure. The titration experiment was performed by addition of one- to eleven-fold aqueous solution of Na 2 [Fe(CN) 5 NO] to dimethyl sulfoxide (DMSO)-phosphate-buffered saline (PBS) (v/v = 1:1) solution of PtAu 2 - 1 cluster (the concentration of 1.0 × 10 -5 M).…”

Section: Methodsmentioning

confidence: 99%

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Chen¹,

Cao²,

Zheng³

et al. 2023

Theranostics

View full text Add to dashboard Cite

Rationale: Inflammatory osteolysis, characterized by abundant immune cell infiltration and osteoclast (OC) formation, is a common complication induced by bacterial products and/or wear particles at the bone-prosthesis interface that severely reduces long-term stability after implantation. Molecular nanoclusters are ultrasmall particles with unique physicochemical and biological properties that have great potential as theranostic agents for treating inflammatory diseases. Methods: In this study, heterometallic PtAu 2 nanoclusters with sensitive nitric oxide-responsive phosphorescence turn-on characteristics and strong binding interactions with cysteine were designed, making them desirable candidates for the treatment of inflammatory osteolysis. Results: PtAu 2 clusters exhibited satisfactory biocompatibility and cellular uptake behavior, with potent anti-inflammatory and anti-OC activities in vitro . In addition, PtAu 2 clusters alleviated lipopolysaccharide-induced calvarial osteolysis in vivo and activated nuclear factor erythroid 2-related factor 2 (Nrf2) expression by disrupting its association with Kelch-like ECH-associated protein 1 (Keap1), thereby upregulating the expression of endogenous anti-inflammatory and anti-oxidative products. Conclusion: Through the rational design of novel heterometallic nanoclusters that activate the endogenous anti-inflammatory system, this study provides new insights into the development of multifunctional molecular therapeutic agents for inflammatory osteolysis and other inflammatory diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Chen¹,

Cao²,

Zheng³

et al. 2023

Theranostics

View full text Add to dashboard Cite

show abstract

“…Furthermore, the substrate specific kinetic parameter is essential to check the performance of the catalyst. [ 40 ] According to the Lineweaver‐Burk plot (Figure S17, Supporting Information), the calculated Michaelis‐Menten constant ( K m ) for Fe‐GDY is 0.14 µ m . [ 41 ] Compared with some previously reported H 2 O 2 sensors constructed with iron‐based catalysts (e.g., Hb enzyme chemically modified with MWCNT and Nafion film, K m = 2 m m [ 42 ] and iron‐chitosan/MWCNT complex, K m = 0.23 m m [ 43 ] ), Fe‐GDY exhibits higher catalytic activity and better binding effect for H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

Boosting Photo‐Electro‐Fenton Process Via Atomically Dispersed Iron Sites on Graphdiyne for InVitro Hydrogen Peroxide Detection

Zheng

et al. 2023

Small

View full text Add to dashboard Cite

Hydrogen peroxide (H2O2) is essential in oxidative stress and signal regulation of organs of animal body. Realizing in vitro quantification of H2O2 released from organs is significant, but faces challenges due to short lifetime of H2O2 and complex bio‐environment. Herein, rationally designed and constructed a photoelectrochemical (PEC) sensor for in vitro sensing of H2O2, in which atomically dispersed iron active sites (Hemin) modified graphdiyne (Fe‐GDY) serves as photoelectrode and catalyzes photo‐electro‐Fenton process. Sensitivity of Fe‐GDY electrode is enhanced 8 times under illumination compared with dark condition. The PEC H2O2 sensor under illumination delivers a wide linear range from 0.1 to 48 160 µm and a low detection limit of 33 nm, while demonstrating excellent selectivity and stability. The high performance of Fe‐GDY is attributed to, first, energy levels matching of GDY and Hemin that effectively promotes the injection of photo‐generated electrons from GDY to Fe3+ for reduced Fe2+, which facilitates the Fe3+/Fe2+ cycle. Second, the Fe2+ actively catalyzes H2O2 to OH‐ through the Fenton process, thereby improving the sensitivity. The PEC sensor demonstrates in vitro quantification of H2O2 released from different organs, providing a promising approach for molecular sensing and disease diagnosis in organ levels.

show abstract

“…During the oxidation process, NO is captured by Ce (IV) in the center of the spatial stereostructure of the CeMOF. The subsequent reaction process is as follows Based on the Lineweaver–Burk plot, the calculated Michaelis–Menten constant ( K m ) of the CeMOF is 1.3 nM (Figure c) and that of CeO 2 is 10.88 nM (Figure S4), while the K m of an iron-based mimetic enzyme NO sensor is 4610 nM, which means that CeMOF as a NO nanozyme with significant catalytic activity exhibits a good binding effect with the substrate. ECSA is used to evaluate the activity of a catalyst, and the value of double-layer capacitance ( C dl ) directly reflects ECSA (Figure S5a,b).…”

Section: Resultsmentioning

confidence: 99%

Nanoporous Ce-Based Metal–Organic Framework Nanoparticles for NO Sensing

Wang

Yin

Rao

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

As a typical reactive nitrogen species, nitric oxide (NO) regulates various physiological processes in tissues. It is challenging to meet the requirements of low detection limit (a few nM) and wide detection range (larger than 1 μM) in detecting NO released from living cells. Herein, we prepare a nanoscale Ce-based metal–organic framework (CeMOF) biomimetic NO sensor, which obtains a high sensitivity (3.10 μA–1 μM–1 cm–2), a long detection range (6.00 nM to 312.67 μM), and a low detection limit (2.00 nM). Further, a CeMOF nanoparticle (NP) screen-printed electrode (SPE) chip is fabricated for cell adhesion and culture. By culturing cells on the surface, the CeMOF NPs/SPE achieves monitoring of NO molecules released from living HeLa and 293A cells. The Michaelis–Menten constant (K m) of the CeMOF biomimetic NO sensor is 1.3 nM, which indicates its excellent catalytic activity, which can be ascribed to the Ce coordination in CeMOF NPs to create high-density active catalytic centers for NO oxidation.

show abstract

Iron-based mimetic enzyme sensor for NO photorelease from sodium nitroprusside

Cited by 8 publications

References 42 publications

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Boosting Photo‐Electro‐Fenton Process Via Atomically Dispersed Iron Sites on Graphdiyne for InVitro Hydrogen Peroxide Detection

Nanoporous Ce-Based Metal–Organic Framework Nanoparticles for NO Sensing

Contact Info

Product

Resources

About

Iron-based mimetic enzyme sensor for NO photorelease from sodium nitroprusside

Cited by 8 publications

References 42 publications

Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Boosting Photo‐Electro‐Fenton Process Via Atomically Dispersed Iron Sites on Graphdiyne for InVitro Hydrogen Peroxide Detection

Nanoporous Ce-Based Metal–Organic Framework Nanoparticles for NO Sensing

Contact Info

Product

Resources

About

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis

Ultrasmall PtAu₂ nanoclusters activate endogenous anti-inflammatory and anti-oxidative systems to prevent inflammatory osteolysis