Iron availability in mixed cultures of sulfate‐reducing bacteria

Hauser, J.; Holder, G. A.

doi:10.1002/bit.260280114

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…to Desulfovibrio cultures resulted in exponential rather than the typical linear growth; these results were generally supported by mixed culture studies performed by Hauser and Holder [6]. Correspondingly, the importance of nanomolar to micromolar iron concentrations has been documented for Desulfovibrio spp., particularly for the synthesis of iron-rich proteins such as cytochrome c3 [13,18].…”

Section: Introductionsupporting

confidence: 72%

The role of iron in enhancing anaerobic toluene degradation in sulfate-reducing enrichment cultures

Beller¹,

Reinhard²

1995

Microb Ecol

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Ferrous iron enhanced the toluene degradation rate of sulfidogenic enrichment cultures inoculated with contaminated subsurface soil from an aviation fuel storage facility near the Patuxent River (Md.). Ferrous iron had an analogous effect on the degradation rate of benzoic acid, a transient metabolite of anaerobic toluene degradation in these cultures, when benzoic acid was used as a sole carbon and energy source. Two hypotheses were proposed to explain iron's effect: (a) Iron may have prevented sulfide toxicity via precipitation of sulfide as FeS, and (b) iron might have been a limiting nutrient required for degradation (i.e., amendments of iron could have compensated for iron removed from solution by precipitation as FeS). To test these hypotheses, substrate degradation rates were compared in the presence of FeSO4 (a sulfate source that both precipitates sulfide species and precludes iron limitation) versus ZnSO4 (a sulfate source that precipitates sulfide species but does not preclude iron limitation) versus MgSO4 (a sulfate source that neither precipitates sulfide nor precludes iron limitation). For both toluene and benzoic acid, FeSO4 and ZnSO4 were comparable in their enhancement of substrate degradation rates and were superior to MgSO4 in that respect. Thus, iron appears to ameliorate sulfide toxicity, not nutritional iron limitation, in these cultures. The observation that ethylenediaminetetraacetic acid, a chelating agent capable of retaining iron in solution in the presence of sulfide, did not stimulate the cultures is consistent with this conclusion. The implications of these results for bioremediation of fuel-contaminated aquifers that contain sulfate-reducing bacteria are discussed.

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Section: Introductionsupporting

confidence: 72%

The role of iron in enhancing anaerobic toluene degradation in sulfate-reducing enrichment cultures

Beller¹,

Reinhard²

1995

Microb Ecol

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“…The culture medium used in all the assays contained a source of essential oligoelements such as B, Co, Cu, Mn and Zn as well as a high concentration of iron, which is consistent with the high requirement of these bacteria for this element. SRB are known to require relatively high levels of iron in culture media in order to compensate for iron precipitation by sulfide (Postgate 1984;Hauser and Holder 1986;Marchal et al 2001). Additionally, metallic elements such as nickel and chromium are present in the chemical composition of stainless steel to improve corrosion resistance.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Desulfovibrio desulfuricans biofilms with stainless steel surface and its impact on bacterial metabolism

et al. 2006

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Aims: To study the influence of some metallic elements of stainless steel 304 (SS 304) on the development and activity of a sulfate‐reducing bacterial biofilm, using as comparison a reference nonmetallic material polymethylmethacrylate (PMMA). Methods and Results: Desulfovibrio desulfuricans biofilms were developed on SS 304 and on a reference nonmetallic material, PMMA, in a flow cell system. Steady‐state biofilms were metabolically more active on SS 304 than on PMMA. Activity tests with bacteria from both biofilms at steady state also showed that the doubling time was lower for bacteria from SS 304 biofilms. The influence of chromium and nickel, elements of SS 304 composition, was also tested on a cellular suspension of Des. desulfuricans. Nickel decreased the bacterial doubling time, while chromium had no significant effect. Conclusions: The following mechanism is hypothesized: a Des. desulfuricans biofilm grown on a SS 304 surface in anaerobic conditions leads to the weakening of the metal passive layer and to the dissolution in the bulk phase of nickel ions that have a positive influence on the sulfate‐reducing bacteria metabolism. This phenomenon may enhance the biocorrosion process. Significance and Impact of the Study: A better understanding of the interactions between metallic surfaces such as stainless steel and bacteria commonly implied in the corrosion phenomena which is primordial to fight biocorrosion.

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“…Sulfate loading rates(4) and conversion efficiency of sulfuric (0) and acetic acid (A) in a 1-L fixed-bed reactor(Fig. 1)with sintered glass beads as carrier material.…”

mentioning

confidence: 99%

Biological sulfuric acid transformation: Reactor design and process optimization

Stucki

Hanselmann

Hürzeler

1993

Biotech & Bioengineering

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As an alternative to the current disposal technologies for waste sulfuric acid, a new combination of recycling processes was developed. The strong acid (H(2)SO(4)) is biologically converted with the weak acid (CH(3)COOH) into two volatile weak acids (H(2)S, H(2)CO(3)) by sulfate-reducing bacteria. The transformation is possible without prior neutralization of the sulfuric acid. The microbially mediated transformation can be followed by physiochemical processes for the further conversion of the H(2)S.The reduction of sulfate to H(2)S is carried out under carbon-limited conditions at pH 7.5 to 8.5. A fixed-bed biofilm column reactor is used in conjunction with a separate gas-stripping column which was installed in the recycle stream. Sulfate, total sulfide, and the carbon substrate (in most cases acetate) were determined quantitatively. H(2)S and CO(2) are continually removed by stripping with N(2). Optimal removal is achieved under pH conditions which are adjusted to values below the pK(a)-values of the acids. The H(2)S concentration in the stripped gas was 2% to 8% (v/v) if H(2)SO(4) and CH(3)COOH are fed to the recycle stream just before the stripping column.Microbiol conversion rates of 65 g of sulfate reduced per liter of bioreactor volume per day are achieved and bacterial conversion efficiencies for sulfate of more than 95% can be maintained if the concentration of undissociated H(2)S is kept below 40 to 50 mg/L. Porous glass spheres, lava beads, and polyurethane pellets are useful matrices for the attachment of the bacterial biomass. Theoretical aspects and the dependence of the overall conversion performance on selected process parameters are illustrated in the Appendix to this article.

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Iron availability in mixed cultures of sulfate‐reducing bacteria

Cited by 15 publications

References 11 publications

The role of iron in enhancing anaerobic toluene degradation in sulfate-reducing enrichment cultures

The role of iron in enhancing anaerobic toluene degradation in sulfate-reducing enrichment cultures

Interaction of Desulfovibrio desulfuricans biofilms with stainless steel surface and its impact on bacterial metabolism

Biological sulfuric acid transformation: Reactor design and process optimization

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