Iron and manganese lipoxygenases of plant pathogenic fungi and their role in biosynthesis of jasmonates

Oliw, Ernst H.

doi:10.1016/j.abb.2022.109169

Cited by 12 publications

(12 citation statements)

References 133 publications

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“…According to Teder et al, FgLOX1 might be involved in jasmonate biosynthesis in F. graminearum; production of fungal jasmonates is supposed as detrimental to plants due to overstimulation of plant jasmonate receptors …”

Section: Discussionmentioning

confidence: 99%

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

Savignac

Atanasova‐Pénichon

Chéreau

et al. 2023

J. Agric. Food Chem.

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Fusarium graminearum is the causal agent of Gibberella ear rot (GER) in maize, a devastating fungal disease resulting in yield reduction and contamination of grains with type B trichothecene (TCTB) mycotoxins. Reducing GER damage requires the implementation of an integrated management strategy in which the use of resistant maize genotypes is a key factor. The present study aimed at providing new phenotyping tools to improve breeding pipelines by investigating the yet understudied contribution of carotenoids to GER resistance. Here, we demonstrated for the first time the efficiency of carotenoid extracts from various maize genotypes to inhibit the production of TCTB by F. graminearum. We further suggested that zeaxanthin could be a key actor of this inhibition efficiency, notably via a negative transcriptional control of several biosynthetic genes of the TCTB pathway. Besides, we demonstrated that zeaxanthin treatments led to profound perturbations in the fungal redox homeostasis by affecting the expression of key genes encoding ROS detoxifying enzymes, several of them being involved in F. graminearum virulence during plant infection. Altogether, our data support the contribution of carotenoids to the mechanisms employed by maize to counteract F. graminearum infection and its production of TCTB.

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Section: Discussionmentioning

confidence: 99%

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

Savignac

Atanasova‐Pénichon

Chéreau

et al. 2023

J. Agric. Food Chem.

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“…Lipoxygenases (LOXs) are a family of enzymes widely represented in plants, animals, fungi and select prokaryotes. [1][2][3] LOXs oxidize polyunsaturated fatty acids to form a diverse array of potent bioactive cell-signaling mediators. For example, 1 the six human LOX isozymes produce a variety of pro-inflammatory oxylipins linked to chronic inflammation, including atherosclerosis, diabetes, stroke, asthma and select cancers.…”

Section: Introductionmentioning

confidence: 99%

“…The lipoxygenase from M. oryzae ( Mo LOX), the focus of this report, is synthesized at the onset of pathogenesis and is secreted by the fungus along with lipases. 3 In vitro studies have shown that Mo LOX oxidizes both polyunsaturated fatty acids and phospholipids, generating unique bis-allylic hydroperoxides ( Scheme 1 ) that are thought to induce oxidative damage and necrosis of rice leaves. 3 , 13 Thus, it has been proposed as one of the prime agents causing the pathology of rice blast disease.…”

Section: Introductionmentioning

confidence: 99%

“…3 In vitro studies have shown that Mo LOX oxidizes both polyunsaturated fatty acids and phospholipids, generating unique bis-allylic hydroperoxides ( Scheme 1 ) that are thought to induce oxidative damage and necrosis of rice leaves. 3 , 13 Thus, it has been proposed as one of the prime agents causing the pathology of rice blast disease. 4 Development of inhibitors for Mo LOX could potentially provide a successful intervention for rice blast disease.…”

Section: Introductionmentioning

confidence: 99%

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¹³C ENDOR Spectroscopy-Guided MD Computations Reveals the Structure of the Enzyme-Substrate Complex of an Active, N-linked Glycosylated Lipoxygenase

Sharma

Whittington

Jabed

et al. 2022

Preprint

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Lipoxygenases (LOXs) are enzymes responsible for producing important cell signaling mediators and have been extensively studied for their potential clinical relevance as well as to advance our understanding of enzyme catalysis. The common inability to capture and characterize LOX-substrate complexes by Xray co-crystallography requires the development of alternative structural methods. We previously reported the integration of13C/1H electron nuclear double resonance (ENDOR) spectroscopy and molecular dynamics (MD) to visualize the complex structure of the paradigmatic LOX from soybean, SLO, with substrate linoleic acid (LA). However, this required substitution of the catalytic mononuclear, nonheme iron by the structurally faithful, yet inactive Mn2+ion as a spin-probe. Unlike canonical Fe-LOXs from plants and animals, LOXs from pathogenic fungi contain active mononuclear manganese metallocentres. Here, we report the ground-state active-site structure of the native, fully glycosylated fungal LOX fromM. oryzae, MoLOX complexed with LA obtained through the13C/1H ENDOR-guided MD approach. The Mn-oxygen-to-LA donor carbon distance (DAD) forMoLOX-LA, 3.4 ± 0.3 Å, matches the distance in the single representative X-ray co-structure of an animal 8R-LOX with its natural fatty acid substrate, and slightly elongated from that of the SLO-LA complex, 3.1 ± 0.2 Å, despite its ‘carboxylate-out’ substrate binding orientation versus ‘carboxylate-in’ for SLO. The results provide unique insight into the evolutionary divergence of the ground-state DAD in the LOX family, which influences the activation barrier for hydrogen tunneling, and give a structural basis for guiding development ofMoLOX inhibitors. The work highlights the robustness of ENDOR-guided MD approach to describe LOX-substrate structures that elude conventional X-ray techniques.

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“…In vascular plants, LOXs were found to be essential for the formation of the phytohormone jasmonoyl isoleucine and are therefore crucial for male fertility, wound response and defense against insects and pathogens among others ( Wasternack and Feussner, 2018 ; Viswanath et al., 2020 ). LOX products were further found to be precursors of green leaf volatiles and divinyl ethers which are involved in defense against herbivores and pathogens ( Christensen and Kolomiets, 2011 ; Oliw, 2022 ). In addition to free PUFAs, some LOXs were also found to accept esterified acyl chains as substrate, for example, the rabbit reticulocyte LOX, cucumber lipid body LBLOX, and Pseudomonas aeruginosa LOX (PaLOX) ( Schewe et al., 1986 ; Feussner et al., 1998 ; Banthiya et al., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa

Djian¹,

Feussner²,

Herrfurth³

et al. 2022

Front. Plant Sci.

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Green microalgae can accumulate neutral lipids, as part of a general lipid remodeling mechanism under stress such as nitrogen starvation. Lobosphaera incisa is of special interest because of its unique TAG acyl chain composition, especially 20:4 (n-6) can reach up to 21% of dry weight after nitrogen starvation. In order to identify factors that may influence the accumulation of polyunsaturated fatty acids (PUFAs), we identified recently a linoleate 13-lipoxygenase (LiLOX). It shares highest identity with plastidic enzymes from vascular plants and is induced upon nitrogen starvation. Here, we confirmed the localization of LiLOX in the stroma of plastids via transient expression in epithelial onion cells. In order to further characterize this enzyme, we focused on the identification of the endogenous substrate of LiLOX. In this regard, an ex vivo enzymatic assay, coupled with non-targeted analysis via mass spectrometry allowed the identification of MGDG, DGDG and PC as three substrate candidates, later confirmed via in vitro assays. Further investigation revealed that LiLOX has preferences towards the lipid class MGDG, which seems in agreement with its localization in the galactolipid rich plastid. Altogether, this study shows the first characterization of plastidic LOX from green algae, showing preference for MGDGs. However, lipidomics analysis did neither reveal an endogenous LiLOX product nor the final end product of MGDG oxidation. Nevertheless, the latter is a key to understanding the role of this enzyme and since its expression is highest during the degradation of the plastidic membrane, it is tempting to assume its involvement in this process.

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Iron and manganese lipoxygenases of plant pathogenic fungi and their role in biosynthesis of jasmonates

Cited by 12 publications

References 133 publications

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

¹³C ENDOR Spectroscopy-Guided MD Computations Reveals the Structure of the Enzyme-Substrate Complex of an Active, N-linked Glycosylated Lipoxygenase

Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa

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Iron and manganese lipoxygenases of plant pathogenic fungi and their role in biosynthesis of jasmonates

Cited by 12 publications

References 133 publications

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

Carotenoids Occurring in Maize Affect the Redox Homeostasis of Fusarium graminearum and Its Production of Type B Trichothecene Mycotoxins: New Insights Supporting Their Role in Maize Resistance to Giberella Ear Rot

13C ENDOR Spectroscopy-Guided MD Computations Reveals the Structure of the Enzyme-Substrate Complex of an Active, N-linked Glycosylated Lipoxygenase

Plastidic membrane lipids are oxidized by a lipoxygenase in Lobosphaera incisa

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¹³C ENDOR Spectroscopy-Guided MD Computations Reveals the Structure of the Enzyme-Substrate Complex of an Active, N-linked Glycosylated Lipoxygenase