Iron absorption in male C282Y heterozygotes

Roe, Mark; Heath, Anne‐Louise M; Oyston, Sarah L.; Macrow, Cate; Hoogewerff, Jurian; Foxall, Robert J.; Dainty, J.; Majsak‐Newman, Gosia; Willis, Gavin; Fairweather‐Tait, Susan J.

doi:10.1093/ajcn/81.4.814

Cited by 44 publications

(33 citation statements)

References 41 publications

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“…Data for thirty (fifteen wild/wild controls, fourteen C282Y/ wild heterozygote) men recruited to take part in an investigation of the effect of HFE genotype on iron absorption (Roe et al 2005) were used to determine the association between serum prohepcidin concentration and iron absorption in healthy men. Meals (high iron bioavailability and fortified cereal products) were labelled extrinsically with stable isotopes of non-haem iron, and absorption was measured from the isotopic enrichment of red blood cells 14 d post-administration using the haemoglobin incorporation technique (Roe et al 2005).…”

Section: Study 1: C282y Heterozygotes and Wild-type Controlsmentioning

confidence: 99%

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Serum prohepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women

et al. 2007

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Serum prohepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women Hepcidin plays a major role in iron homeostasis, but understanding its role has been hampered by the absence of analytical methods for quantification in blood. A commercial ELISA has been developed for serum prohepcidin, a hepcidin precursor, and there is interest in its potential use in the clinical and research arena. We investigated the association between serum prohepcidin concentration and iron absorption in healthy men, and its relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients, and pregnant women. Iron absorption was determined in thirty healthy men (fifteen wild-type, fifteen C282Y heterozygote) using the stable isotope red cell incorporation technique. Iron status was measured in 138 healthy men (ninety-one wild-type, forty-seven C282Y heterozygote), six hereditary haemochromatosis patients, and thirteen pregnant women. Mean serum prohepcidin concentrations were 214 (SD 118) ng/ml [208 (SD 122) ng/ml in wild-type and 225 (SD 109) ng/ml in C282Y heterozygotes] in healthy men, 177 (SD 36) ng/ml in haemochromatosis patients, and 159 (SD 59) ng/ml in pregnant women. There was no relationship between serum prohepcidin concentration and serum ferritin in any subject groups, nor was it associated with efficiency of iron absorption. Serum prohepcidin is not a useful biomarker for clinical or research purposes. Hepcidin plays a major role in iron homeostasis, but full characterisation of its role in healthy humans and patients has been hindered by the absence of analytical methods to quantify circulating levels in the blood. Quantification of mRNA in the liver has been undertaken using reverse transcription and the polymerase chain reaction (Bridle et al. 2003;Gehrke et al. 2003), and polyclonal antibodies to refolded synthetic hepcidin have been produced and used for quantification in urine (Nemeth et al. 2003). Attempts to produce correctly folded synthetic hepcidin have proved difficult because the sequence contains eight cysteine residues that constrain the hepcidin molecule in a hairpin structure (Hunter et al. 2002). Hepcidin in urine has also been quantified using mass spectrometry methods (Kemna et al. 2005;Liang et al. 2006;Tomosugi et al. 2006).Prohepcidin, the sixty-amino-acid product of cleavage of the signal peptide from the hepcidin precursor, is expressed at the basolateral membrane domain of hepatocytes and is found in blood (Kulaksiz et al. 2004). Serum prohepcidin concentrations are significantly lower in patients with hereditary haemochromatosis compared to healthy control subjects (Kulaksiz et al. 2004), increase with declining kidney function (Taes et al. 2004), and are correlated with haematocrit in chronic haemodialysis patients (Hsu et al. 2006), but it is currently unclear whether * Corresponding author: ...

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Section: Study 1: C282y Heterozygotes and Wild-type Controlsmentioning

confidence: 99%

“…Meals (high iron bioavailability and fortified cereal products) were labelled extrinsically with stable isotopes of non-haem iron, and absorption was measured from the isotopic enrichment of red blood cells 14 d post-administration using the haemoglobin incorporation technique (Roe et al 2005).…”

Section: Study 1: C282y Heterozygotes and Wild-type Controlsmentioning

confidence: 99%

Serum prohepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women

et al. 2007

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“…Fourteen days after the labeled meal was consumed, a blood sample was taken and iron absorption from the test meal was determined by measuring the enrichment of Fe-57 and Fe-58 in red blood cells following the method by Roe et al [13]. Absorption was calculated from the enrichment of isotopes in the blood, the total iron concentration in whole blood and an estimate of blood volume [14].…”

Section: Iron Absorption Studymentioning

confidence: 99%

The G277S transferrin mutation does not affect iron absorption in iron deficient women

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“…More recently, iron absorption from fortified cereals and a high iron bioavailability meal was measured in C282Y heterozygotes and found to be similar to that of wildtype controls [20]. Carriers also exhibit no difference in the efficiency of haem iron absorption [13].…”

Section: Diet Genotype and Phenotypementioning

confidence: 99%

Effect of SNPs on iron metabolism

et al. 2007

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Iron absorption in male C282Y heterozygotes

Abstract: There was no overall significant difference between C282Y heterozygotes and wild-type men in iron absorption from either dietary nonheme iron or fortified cereal products.

Cited by 44 publications

References 41 publications

Serum prohepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women

Serum prohepcidin concentration: no association with iron absorption in healthy men; and no relationship with iron status in men carrying HFE mutations, hereditary haemochromatosis patients undergoing phlebotomy treatment, or pregnant women

The G277S transferrin mutation does not affect iron absorption in iron deficient women

Effect of SNPs on iron metabolism

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