Iridoids from Gentiana loureirii

Wu, Min; Wu, Ping; Liu, Meifang; Xie, Haihui; Jiang, Yueming; Wei, Xiaoyi

doi:10.1016/j.phytochem.2009.03.018

Cited by 32 publications

(25 citation statements)

References 23 publications

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“…These compounds have been isolated from many plants, and many of their bioactivities, including anti-inflammatory, anti-allergic, and antiasthmatic effects, have been reported. [16][17][18][19] However, this is the first report of their allergy-preventive effects in the induction phase of an allergy. Among them chlorogenic acid (1) is regarded as an active substance of LJ, since 1 is a major constituent of the extract.…”

Section: Discussionmentioning

confidence: 99%

Allergy-Preventive Effects of Chlorogenic Acid and Iridoid Derivatives from Flower Buds of Lonicera japonica

Oku

Ogawa

Iwaoka

et al. 2011

Biological & Pharmaceutical Bulletin

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Flower buds of Lonicera japonica THUNB. (Caprifoliaceae), one of the most common traditional Chinese medicines, are used to treat various diseases including arthritis, diabetes mellitus, fever, infections, sores and swelling. 1)Pharmacological studies have indicated that the extract of these flower buds have a broad spectrum of biological activities, including antibacterial, anti-inflammatory, antioxidant, antipyretic, antiviral and hepato-protective effects.1,2) A number of chemical constituents including flavonoids, iridoids and saponins have been obtained from this plant. [3][4][5][6][7][8] In our continuing search for allergy-preventive substances from natural sources, 9) we have been using our previously developed in vivo assay method to estimate effects on complex allergies. 10,11) This time, we found that the 35% EtOH extract of flower buds of L. japonica exhibited allergy-preventive activity. Our in vivo assay method monitors the decrease in blood flow (BF) in the tail vein of mice subjected to hen eggwhite lysozyme (HEL) sensitization alone without the HELchallenge as a guide.10) The BF in HEL-sensitized mice (control group) gradually and significantly decreased to about 70% of that in normal mice on day 9. Thus, the induction phase (promotion stage) of allergy caused by xenobiotics can be dynamically and easily measured using BF monitoring. This BF decrease is considered to be due to the contraction of peripheral blood vessels and an increase in blood viscosity, because no relationship with blood pressure was observed. Although anti-HEL immunoglobulin E (IgE) antibody significantly increased after HEL sensitization, there was no significant increase in the number of leukocytes. Thus, a decrease in BF reflects the promoter process of an allergic reaction. The BF decrease is regulated by various factors such as nitric oxide (NO), thromboxane (TX) A 2 , prostacyclin (PGI 2 ), and endothelin (ET)-1, together with granulocytic elastase (GE), cyclooxygenase (COX)-1 and -2, inducible nitric oxide synthase (iNOS), and constitutive nitric oxide synthase (cNOS).10) In addition, the BF decrease occurs via both pathways of iNOS-independent and -dependent responses.11) Therefore this monitoring system should be useful when searching for substances that can prevent complicated inflammatory allergies involving NO, TXA 2 , PGI 2 , ET-1, GE, COX-1, and COX-2. This paper describes the evaluation of the allergy-preventive effects of a 35% EtOH extract (LJ) of flower buds of L. japonica and the following compounds isolated from LJ: chlorogenic acid (1) and three iridoid derivatives, loganin (2), secoxyloganin (3) and sweroside (4).The structure-activity relationships of iridoid derivatives, morroniside (5), geniposide (6), asperuloside (7), aucubin (8) and catalpol (9) were also examined, as iridoid glucosides (2-4) isolated from LJ showed allergy-preventive effects. MATERIALS AND METHODS General Experimental ProceduresMelting points were determined on a Yanagimoto micro-point apparatus. IR spectra were recorded on a Shim...

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Section: Discussionmentioning

confidence: 99%

Allergy-Preventive Effects of Chlorogenic Acid and Iridoid Derivatives from Flower Buds of Lonicera japonica

Oku

Ogawa

Iwaoka

et al. 2011

Biological & Pharmaceutical Bulletin

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“…2a, b). It is known that the predominant compounds in R. glutinosa roots are iridoid glucosides, which are a group of compounds with poor antioxidant properties (Es-Safi et al 2007;Wu et al 2009). Table 4 showed significant linear correlations between total phenolic and flavonoid levels and the antioxidant capacities of the R. glutinosa extracts tested.…”

Section: Shoot Rooting and Plant Acclimatizationmentioning

confidence: 99%

Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

2014

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Rehmannia glutinosa Libosch., a valuable medicinal plant, was successfully propagated in vitro using shoot tip explants. Shoot multiplication was performed in glass tubes and in a nutrient sprinkle bioreactor. A mixture of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 of 6-benzylaminopurine in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 8.2 shoots per explant after 4 weeks of culture in glass tubes. The number of shoots increased to 21 per explant when the same combination of growth regulators was used in a nutrient sprinkle bioreactor. The shoots rooted with a frequency of 93 % after 6 weeks of culture on MS agar medium supplemented with IAA (0.1 mg L -1 ) before being acclimatized in the greenhouse. The antioxidant activities of methanolic extracts from the leaves and roots of the in vitro-regenerated plants of R. glutinosa cultivated in the greenhouse were evaluated using four in vitro assays: scavenging of free radicals (DPPH and ABTS), transition metal reduction and total antioxidant activity phosphomolybdenum test. In all cases, the methanolic extract from leaves demonstrated better antioxidant activity than those taken from roots. A strong correlation was found between total phenolic and flavonoid content, and the antioxidant capacity of the studied extracts.

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“…These plants are widely used in traditional medicine in many countries for stimulation of appetite and gastric secretion, gastro-duodenal protection, liver protection, antifungal treatment, and in some cases for gynaecological diseases [13]. Experimental studies have showed, that Gentiana contains as main components compounds such as secoiridoid-glycosides (gentiopicroside, sweroside, swertiamarin, amarogentin), xanthone glycosides (gentioside and its isomer), terpenes, xanthones (gentisin, isogentisin), secoiridoids (gentiopicrine), alkaloids (gentianine, gentiabetin, gentianidine, gentialutin), anthocyanins (delphinidine) and flavone C-glucosides [14][15][16][17][18][19][20]. Iridoids are the most common constituents of genus Gentiana, and they have been found to exhibit a wide range of bioactivities including antibacterial, anticancer, antifungal, antioxidant, anti-inflammatory, anticoagulant, choleretic, cardioprotective, hepatoprotective, neuroprotective, hypoglycaemic, antispasmodic, immunomodulatory and purgative properties [16,[21][22].…”

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confidence: 99%

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Hudecová¹,

Hašplová²,

Kellovska³

et al. 2012

neo

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Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single-and double-strand breaks, as well as a DNA base loss resulting in apurinic/apyrimidinic (AP) sites. The aim of this study was to induce an adaptive response (AR) by zeocin in freshly isolated human lymphocytes from blood and to observe whether plant extracts could modulate this response. The AR was evaluated by the comet assay. The optimal conditions for the AR induction and modulation were determined as: 2 h-intertreatment time (in PBS, at 4°C) given after a priming dose (50 µg/ml) of zeocin treatment. Genotoxic impact of zeocin to lymphocytes was modulated by plant extracts isolated from Gentiana asclepiadea (methanolic and aqueous haulm extracts, 0.25 mg/ml) and Armoracia rusticana (methanolic root extract, 0.025 mg/ml). These extracts enhanced the AR and also decreased DNA damage caused by zeocin (after 0, 1 and 4 h-recovery time after the test dose of zeocin application) to more than 50%. These results support important position of plants containing many biologically active compounds in the field of pharmacology and medicine.

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Iridoids from Gentiana loureirii

Cited by 32 publications

References 23 publications

Allergy-Preventive Effects of Chlorogenic Acid and Iridoid Derivatives from Flower Buds of Lonicera japonica

Allergy-Preventive Effects of Chlorogenic Acid and Iridoid Derivatives from Flower Buds of Lonicera japonica

Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

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