Iridin Attenuates the Migration and Attachment Independent Growth of Lung Adenocarcinoma Cells

Abstract

Lung adenocarcinoma is the leading cause of lung cancer related deaths worldwide. The low survival rates associated with this cancer are being attributed to metastasis and recurrence which contribute to the failure of treatment. In this study we studied the ability of Iridin, anisoflavone isolated from Iris Kashmirianato ablate the metastatic progression in human lung adenocarcinoma. Human lung adenocarcinoma cells, A549 were treated with various concentrations of Iridin for 24 h to evaluate their effect on their proliferation by MTT assay. Only those concentrations which showed least effect on the proliferation of A549 cells were used to examine their effect on migration and attachment independence. The effect of such concentrations on the migration and attachment independence of A549 cells was determined by wound healing and soft agar colony formation assays respectively. The results demonstrated that treatment with Iridinled to significant inhibition in the migration as well as colony formation in lung adenocarcinoma cells. Taken together, these findings present a preliminary account of anti-metastatic activity of Iridin which warrants further investigation to decipher its mechanistic action.