Irbesartan Ameliorates Myocardial Ischaemia/Reperfusion Injury in Rats Via Down Regulation of Apoptosis and the Inflammatory Pathways

Hadi, Najah R.; Al-Amran, Fadhil G.; Mohammad, Bassim I; Zamil, Suhad T

doi:10.1016/j.jacc.2013.08.182

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By apoptosis imaging with 99m Tc-annexin A5, Meijer et al demonstrated that irbesartan does not induce apoptosis in transient ischemia-reperfusion in humans [22] . A recent animal study indicated that irbesartan can ameliorate the myocardial ischemia/reperfusion injury via down-regulation of apoptosis [23] . Thus, the above mentioned previous findings are inconsistent, and no previous study has directly examined the effects of ARBs on apoptosis in atherosclerotic plaques.…”

Section: Discussionmentioning

confidence: 99%

Suppressive Effects of Irbesartan on Inflammation and Apoptosis in Atherosclerotic Plaques of apoE−/− Mice: Molecular Imaging with 14C-FDG and 99mTc-Annexin A5

et al. 2014

View full text Add to dashboard Cite

ObjectivesTo investigate the effects of irbesartan on inflammation and apoptosis in atherosclerotic plaques by histochemical examination and molecular imaging using 14C-FDG and 99mTc-annexin A5.BackgroundIrbesartan has a peroxisome proliferator-activated receptor gamma (PPARγ) activation property in addition to its ability to block the AT1 receptor. Accordingly, irbesartan may exert further anti-inflammatory and anti-apoptotic effects in atherosclerotic plaques. However, such effects of irbesartan have not been fully investigated. Molecular imaging using 18F-FDG and 99mTc-annexin A5 is useful for evaluating inflammation and apoptosis in atherosclerotic plaques.MethodsFemale apoE−/− mice were treated with irbesartan-mixed (50 mg/kg/day) or irbesartan-free (control) diet for 12 weeks (n = 11/group). One week after the treatment, the mice were co-injected with 14C-FDG and 99mTc-annexin A5, and cryostat sections of the aortic root were prepared. Histochemical examination with Movat's pentachrome (plaque size), Oil Red O (lipid deposition), Mac-2 (macrophage infiltration), and TUNEL (apoptosis) stainings were performed. Dual-tracer autoradiography was carried out to evaluate the levels of 14C-FDG and 99mTc-annexin A5 in plaques (%ID×kg). In vitro experiments were performed to investigate the mechanism underlying the effects.ResultsHistological examination indicated that irbesartan treatment significantly reduced plaque size (to 56.4%±11.1% of control), intra-plaque lipid deposition (53.6%±20.2%) and macrophage infiltration (61.9%±20.8%) levels, and the number of apoptotic cells (14.5%±16.6%). 14C-FDG (43.0%±18.6%) and 99mTc-annexin A5 levels (45.9%±16.8%) were also significantly reduced by irbesartan treatment. Irbesartan significantly suppressed MCP-1 mRNA expression in TNF-α stimulated THP-1 monocytes (64.8%±8.4% of un-treated cells). PPARγ activation was observed in cells treated with irbesartan (134%±36% at 3 µM to 3329%±218% at 81 µM) by a PPARγ reporter assay system.ConclusionsRemissions of inflammation and apoptosis as potential therapeutic effects of irbesartan on atherosclerosis were observed. The usefulness of molecular imaging using 18F-FDG and 99mTc-annexin A5 for evaluating the therapeutic effects of irbesartan on atherosclerosis was also suggested.

show abstract

Section: Discussionmentioning

confidence: 99%

Suppressive Effects of Irbesartan on Inflammation and Apoptosis in Atherosclerotic Plaques of apoE−/− Mice: Molecular Imaging with 14C-FDG and 99mTc-Annexin A5

et al. 2014

View full text Add to dashboard Cite

show abstract

“…During the I/R injury, boosted the hs-CRP level, which is closely related to the prognosis, severity and occurrence of atherosclerosis and acute cerebral infraction and it is considered as an important biomarker of cardiovascular disease ( Yi et al, 2019 , Feng et al, 2020 ). During the I/R injury, start the secretion of hs-CRP into the circulation which further increases the atheromatous plaques and instability ( Ito et al, 2003 , Hadi et al, 2013 ). In this study, I/R injury rats exhibited the boosted level of MCP-1 and hs-CRP and sinomenine considerably suppressed the level.…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective effects of sinomenine in myocardial ischemia/reperfusion injury in a rat model

Lu¹,

X²,

et al. 2022

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Anti-apoptotic Effect of MiR-223-3p Suppressing PIK3C2A in Cardiomyocytes from Myocardial Infarction Rat Through Regulating PI3K/Akt Signaling Pathway

et al. 2021

View full text Add to dashboard Cite

Irbesartan Ameliorates Myocardial Ischaemia/Reperfusion Injury in Rats Via Down Regulation of Apoptosis and the Inflammatory Pathways

Cited by 3 publications

References 0 publications

Suppressive Effects of Irbesartan on Inflammation and Apoptosis in Atherosclerotic Plaques of apoE−/− Mice: Molecular Imaging with 14C-FDG and 99mTc-Annexin A5

Suppressive Effects of Irbesartan on Inflammation and Apoptosis in Atherosclerotic Plaques of apoE−/− Mice: Molecular Imaging with 14C-FDG and 99mTc-Annexin A5

Cardioprotective effects of sinomenine in myocardial ischemia/reperfusion injury in a rat model

Anti-apoptotic Effect of MiR-223-3p Suppressing PIK3C2A in Cardiomyocytes from Myocardial Infarction Rat Through Regulating PI3K/Akt Signaling Pathway

Contact Info

Product

Resources

About