IR spectroscopic characteristics of cell cycle and cell death probed by synchrotron radiation based Fourier transform IR spectromicroscopy

Holman, Hoi‐Ying N.; Martin, Michael; Blakely, Eleanor A.; Bjornstad, Kathleen A.; McKinney, Wayne R.

doi:10.1002/1097-0282(2000)57:6<329::aid-bip20>3.0.co;2-2

Cited by 213 publications

(158 citation statements)

References 19 publications

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“…Absorptions in this spectral region arise from the carbonyl C ¼ O stretching mode of phospholipids. 27,28 It has been described that upon washing with ethanol, phospholipids together with other membrane lipids are removed from the sample and thus their intensity decreases in the spectrum. 15 This corroborates the changes observed above for the CH 2 modes.…”

Section: Discussionmentioning

confidence: 99%

Synchrotron-based FTIR spectra of stained single cells. Towards a clinical application in pathology

Pijanka

Sockalingum

Köhler

et al. 2010

Laboratory Investigation

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Over the last few years, FTIR spectroscopy has become a potential analytical method in tissue and cell studies for cancer diagnosis. This has opened a way towards clinical applications such as a tool that would scan samples to assess the presence or absence of malignant cells in biopsies, or as an aid to help pathologists to better characterise those cells that are suspicious but not diagnostic for cancer. The latter application has the problem that in order to assess these cells pathologists would have already dealt with stained samples. Therefore, it is important to understand how staining would affect the spectra of cells. To this purpose, we have conducted this study in order to clarify, first, how haematoxylin and eosin (H&E) and Papanicolau (Pap) stainings affect the spectra of single cells and, second, whether FTIR spectroscopy could differentiate between stained lung cancer cells and their normal counterparts. Furthermore, different cell preparations (cytospin, and smear) used in cytological diagnosis were assessed. Experiments performed using a bright infrared (IR) source (synchrotron) showed that both H&E and Pap staining induced marked changes in the lipid and amide-II band regions. Despite this, FTIR spectroscopy of already stained cells is capable of differentiating between lung cancer cells and their normal counterparts. The clinical applications of this methodology are discussed.

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Section: Discussionmentioning

confidence: 99%

Synchrotron-based FTIR spectra of stained single cells. Towards a clinical application in pathology

Pijanka

Sockalingum

Köhler

et al. 2010

Laboratory Investigation

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“…At present Fourier Transform Infrared Microspectrosopy (FTIRM) and Confocal Raman Microspectroscopy (CRM) have identified in-situ molecular alterations associated with cell death via apoptosis [1][2][3] or necrosis [4,5], or as a result of proliferative changes in cellular activity [6][7][8] or mitosis [9]. It has also been shown that these modalities can identify molecular changes associated with changes in the phenotype of differentiating embryonic cells [10,11].…”

Section: Introductionmentioning

confidence: 99%

Growth substrate induced functional changes elucidated by FTIR and Raman spectroscopy in in–vitro cultured human keratinocytes

Meade¹,

Lyng²,

Knief³

et al. 2006

Anal Bioanal Chem

109

115

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“…Some of the important considerations already highlighted for IR spectroscopy were also highlighted in this study, particularly the importance of performing Mie scattering correction. Significant differences in Mie scattering contribution between the different cell cycle stages were observed, likely due to changes in amount of cellular content [65], and therefore the RMieS-EMSC correction algorithm [40] was used. The ability to detect changes after 24 hr treatment was highlighted as an advantage with respect to HTS, and the benefit of distinguishing cell cycle without additional staining was confirmed as an ideal next step.…”

Section: Infrared Spectroscopy For Monitoring Drug-cell Interactionmentioning

confidence: 99%

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Jamieson

Byrne²

2017

Vibrational Spectroscopy

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. (2017). Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? Vibrational Spectroscopy, vol. 91, July, pp. 16-30. doi.org/10.1016/ j.vibspec.2016 Vibrational spectroscopy as a tool for studying drug-cell interaction: could high throughput vibrational spectroscopic screening improve drug development? AbstractVibrational spectroscopy is currently widely explored as a tool in biomedical applications. An area at the forefront of this field is the use of vibrational spectroscopy for disease diagnosis, ultimately aiming towards spectral pathology. However, while this field shows promising results, moving this technique into the clinic faces the challenges of widespread clinical trials and legislative approval. While spectral pathology has received a lot of attention, there are many other biomedical applications of vibrational spectroscopy, which could potentially be translated to applications with greater ease. A particularly promising application is the use of vibrational spectroscopic techniques to study the interaction of drugs with cells. Many studies have demonstrated the ability to detect biochemical changes in cells in response to drug application, using both infrared and Raman spectroscopy. This has shown potential for use in high throughput screening (HTS) applications, for screening of efficacy and mode of action of potential drug candidates, to speed up the drug discovery process. HTS is still a relatively new and growing area of research and, therefore, there is more potential for new techniques to move into and shape this field. Vibrational spectroscopic techniques come with many benefits over the techniques used currently in HTS, primarily based on fluorescence assays to detect specific binding interactions or phenotypes. They are label free, and an infrared or Raman spectrum provides a wealth of biochemical information, and therefore could reveal not only information about a specific interaction, but about how the overall biochemistry of a cell changes in response to application of a drug candidate. Therefore, drug mode of action could be elucidated. This review will investigate the potential for vibrational spectroscopy, particularly FTIR and Raman spectroscopy, to benefit the field of HTS and improve the drug development process. In addition to FTIR and Raman spectroscopy, surface enhanced Raman spectroscopy (SERS), coherent anti-Stokes Raman spectroscopy (CARS) and stimulated Raman spectroscopy (SRS), will be investigated as an alternative tool in the HTS process.

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IR spectroscopic characteristics of cell cycle and cell death probed by synchrotron radiation based Fourier transform IR spectromicroscopy

Cited by 213 publications

References 19 publications

Synchrotron-based FTIR spectra of stained single cells. Towards a clinical application in pathology

Synchrotron-based FTIR spectra of stained single cells. Towards a clinical application in pathology

Growth substrate induced functional changes elucidated by FTIR and Raman spectroscopy in in–vitro cultured human keratinocytes

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

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