Abnormal cholesterol concentrations are related to dyslipidemia, cardiovascular disease, anemia, hypothyroidism, cerebral hemorrhages, and cancer. [2] The primary sources of human cholesterol are diet and synthesis; thus, cholesterol levels can represent the diet and serum spectrum, and are important in food quality control and clinical diagnosing. Various methods, such as colorimetric, enzymatic, fluorometric, chromatographic, and electrochemical approaches, have been proposed for cholesterol quantification. [3,4] Among those methods, indirect cholesterol quantification through an optical analysis of the hydrogen peroxide (H 2 O 2 ) produced by cholesterol oxidation catalyzed by cholesterol oxidase (ChO x ) has proved to be popular and effective. [5,6] In the human body, H 2 O 2 production occurs in response to various enzymatic reactions, and thus it has been suggested as a potential biomarker. [7] Apart from its well-known cytotoxic effects, H 2 O 2 plays a crucial role in many physiological and biological processes, and has significant applications in textiles, cosmetics, agriculture, heavy industry, room decontamination, and food processing. [8] The conventional optical detection methods use natural enzymes (such as horseradish peroxide, HRP), which catalyze H 2 O 2 and produce hydroxyl radicals that oxidize specific substrates and generate readable signals. [3,[9][10][11][12] Although natural enzymes have the advantages of high sensitivity and selectivity, they suffer from complicated immobilization mechanisms, high costs, slow catalytic activity, and low reliability under harsh environmental conditions. [13] When they are used in sensing methods, natural enzymes are particularly vulnerable to interfering substances. [13,14] Therefore, the use of artificial enzymes has gained considerable interest because of their ability to overcome the shortcomings of natural enzymes. [15][16][17][18][19][20][21][22] Since the first artificial enzyme (Fe 3 O 4 ) was discovered, a range of materials has been found to have intrinsic enzymelike activity, including noble metals, transition metals/metal oxides, carbon variants, composites, and metal-organic frameworks. [23][24][25][26] Nanozymes based on metal-carbon hybrids have proven to be particularly effective. [27] Bao et. al. prepared a Artificial enzymes have attracted considerable attention because of their significant advantages over natural enzymes. Herein, the authors report a simple hydrothermal strategy for producing a bimetallic metal -N-, S-doped carbon (NS-C@PtRu) hybrid derived from L-cysteine-mediated self-assembly of PtRu. The peroxidase-like activity and colloidal stability of the NS-C@PtRu are tuned by varying the amount of L-cysteine, the source material for the N-, S-doped carbon. The NS-C@PtRu exhibits good dispersibility, synergistic interactions (metal-metal, metal-carbon), non-metal (N, S) doping, more catalytic active sites, improved noble metallic catalyst performance, and high utilization efficiency. As an excellent peroxidase mimic, NS-C@PtRu ca...