Ipsilateral common iliac artery plus femoral artery clamping for inducing sciatic nerve ischemia/reperfusion injury in rats: a reliable and simple method

Nouri, Mohsen; Rahimian, Reza; Fakhfouri, Gohar; Mohammadi-Rick, Sanaz; Barzegar-Fallah, Anita; Asadi-Amoli, Fahimeh; Dehpour, Ahmad Reza

doi:10.1186/1749-7221-3-27

Cited by 7 publications

(4 citation statements)

References 7 publications

(9 reference statements)

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“…The rat model of SN I/R injury was established as described in a previous study ( 33 ). Briefly, the rats were fasted for 12 h, with ad libitum access to water, prior to experiments.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

Chen

2016

Experimental and Therapeutic Medicine

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Inhibition of the N-methyl-D-aspartate (NMDA) receptor by MK-801 reduces ischemia/reperfusion (I/R) injury in the central nervous system. However, few previous studies have evaluated the neuroprotective effects of MK-801 against peripheral I/R injury. The present study aimed to investigate the protective effects of MK-801 pretreatment against I/R injury in the rat sciatic nerve (SN). Sprague-Dawley rats were subjected to a sham surgery (n=8) or to a 5-h ischemic insult by femoral artery clamping (I/R and I/R+MK-801 groups; n=48 per group). I/R+MK-801 rats were intraperitoneally injected with MK-801 (0.5 ml or 1 mg/kg) at 15 min prior to reperfusion. The rats were sacrificed at 0, 6, 12, 24, 72 h, or 7 days following reperfusion. Plasma malondialdehyde (MDA) and nitric oxide (NO) concentrations, and SN inducible NO synthase (iNOS) protein expression levels, were measured using colorimetry. In addition, the protein expression levels of tumor necrosis factor-α (TNF-α) were measured using immunohistochemistry, and histological analyses of the rat SN were conducted using light and electron microscopy. Alterations in the mRNA expression levels of TNF-α and TNF-α converting enzyme (TACE) in the rat SN were detected using reverse transcription-quantitative polymerase chain reaction. In the I/R group, plasma concentrations of NO (175.3±4.2 µmol/l) and MDA (16.2±1.9 mmol/l), and the levels of iNOS (2.5±0.3) in the SN, peaked at 24 h post-reperfusion. At 24 h, pretreatment with MK-801 significantly reduced plasma NO (107.3±3.6 µmol/l) and MDA (11.8±1.6 mmol/l), and SN iNOS (1.65±0.2) levels (all P<0.01). The mRNA expression levels of TNF-α and TACE in the SN were significantly reduced in the I/R+MK-801 group, as compared with the I/R group (P<0.05). Furthermore, MK-801 pretreatment was shown to have alleviated histological signs of I/R injury, including immune cell infiltration and axon demyelination. The results of the present study suggested that pretreatment with MK-801 may alleviate I/R injury of the SN by inhibiting the activation of TNF-α and reducing the levels of iNOS in the SN.

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“…The rat model of SN I/R injury was established as described in a previous study ( 33 ). Briefly, the rats were fasted for 12 h, with ad libitum access to water, prior to experiments.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

Chen

2016

Experimental and Therapeutic Medicine

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“…Novel neuropathic pain model has been proposed in complex regional pain syndrome (CRPS) produced by prolonged hindpaw ischemia and reperfusion in rat [ 8 ]. Ischemic-reperfusion event is well documented to induce potent injury in the targeted organs, which were indicated in the myocardial, renal, liver, lung, stomach and neuronal cells [ 9 - 11 ]. Ischemic-reperfusion process leads to change in the microvascular environment which in turn causes neuronal edema, breakdown of blood-nerve barrier, nerve fiber degeneration, neuronal excitation, decreased nerve conduction velocity, membranous lipid peroxidation, accumulation of free radical, alteration of enzymatic reaction, ion fluxes etc [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Pharmacological evaluation of tacrolimus (FK-506) on ischemia reperfusion induced vasculatic neuropathic pain in rats

Muthuraman

Sood

2014

J Brachial Plex Peripher Nerve Inj

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BackgroundIschemia reperfusion (I/R) is common in various pathological conditions like diabetic complication, rheumatic arthritis, necrotizing vascular occlusive disease and trauma.MethodsWe have evaluated the effect of tacrolimus (1, 2 and 3 mg/kg, p.o. for 10 consecutive days) on femoral arterial ischemic reperfusion (I/R) induced neuropathic pain in rats. Behavioral parameters (i.e. hot plate, radiant heat, acetone drop, tail heat hyperalgesia, tail flick and tail cold allodynia tests) were assessed at different time intervals (i.e. 0, 1, 4, 7, 10, 13 and 16th day) and biochemical analysis in serum and tissue samples were also performed along with histopathological studies.ResultsBehavioral pain assessment revealed increase in the paw and tail withdrawal threshold in tacrolimus treated groups against hyperalgesic and allodynic stimuli as compared to the sham control group. We observed a decrease in the serum nitrate and thiobarbituric acid reactive substance (TBARS) levels along with reduction in tissue myeloperoxidase (MPO) and total calcium levels, whereas, rise in tissue reduced glutathione levels in tacrolimus treated groups. However, significant results were obtained in medium and high dose treated group as compared to sham control group. Histopathological study had revealed the increase in the neuronal edema and axonal degeneration in the I/R group whereas, tacrolimus ameliorate these effects.ConclusionOur results indicate the anti-oxidative, anti-inflammatory and calcium modulatory actions of tacrolimus. Therefore, it can be used as a therapeutic agent for the treatment of vascular inflammatory related neuropathic pain.

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“…It is clinically used for the treatment of asthma by reducing the eosinophilic inflammation in the airways (7,41). Anti-inflammatory and antioxidant protective effects of montelukast against I/R injury of kidneys, ovaries, liver, brain and skin flaps have recently been shown in several experimental studies (6,19,24,25,31,42). Therefore, the present study was designed to investigate the role of montelukast on spinal cord I/R injury in an experimental model.…”

Section: Korkmaz K Et Al: Montelukast and Spinal Cord Injurymentioning

confidence: 99%

Effect of montelukast on spinal cord ischemia/reperfusion injury

Korkmaz¹,

Gedik²,

Budak³

et al. 2014

Turkish Neurosurgery

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AIm: Paraplegia due to ischemia-reperfusion (I/R) injury of the spinal cord is a devastating complication of thoracoabdominal aortic surgery. Cysteinyl leukotrienes are potent mediators of inflammation that are associated with I/R injury. The present study was designed to investigate the role of montelukast, a selective reversible CysLT1 receptor antagonist, on spinal cord I/R injury in an experimental model. mAterIAl and methOds: Twenty-one male Sprague-Dawley rats were randomly assigned to three groups (n=7 per group) as G1 (no aortic occlusion and montelukast administration), G2 (45 min. aortic occlusion; no montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast administration). After neurologic evaluation using the Motor Deficit Index (MDI) score at the 48th hour of reperfusion, lumbar spinal cords were removed for histopathological evaluation and immunohistochemical staining for HSP70, interleukin-6 and myeloperoxidase (MPO).results: All rats in the G1 group had a normal neurological status and their MDI score was 0 (p<0.05). The MDI score of G3 was significantly lower than G2 group (2.8 vs. 5.5; p<0.05). Vacuolar congestion was found to be significantly lower in G1 than the other groups (p=0.0001). The interleukin-6 receptor level was found to be significantly lower in G3 group than the control group (p=0.013). There was no statistically significant difference found among the groups in terms of the degree of HSP70 and MPO staining.COnClusIOn: Increased generation of leukotrienes in postischemic organs play an important role in I/R injury. The findings of the current study demonstrated that montelukast improved motor recovery and decreased IL-6 levels in spinal cord I/R injury. KeywOrds:Spinal cord ischemia, Montelukast, Reperfusion injury, Rat, Experimental, CysLT1 receptor ÖZ AmAÇ: Çalışma, deneysel bir modelde, seçici tersinir bir CysLT1 reseptör antagonisti olan montelukastın, spinal kord iskemi-reperfüzyon (I/R) hasarı üzerindeki rolünü incelemek için tasarlandı. yÖntem ve GereÇler: Yirmi bir erkek Sprague-Dawley tipi sıçan, rasgele üç gruba (her grupta n = 7) ayrıldı; G1 (aort oklüzyonu ve montelukast uygulaması yok), G2 (45 dakika aort oklüzyonu; montelukast uygulaması yok) ve G3 (45 dk aort oklüzyonu, 10 mg / kg montelukast uygulaması). Reperfüzyonun 48. saatindeki Motor Defisit Indeksi (MID) skorlaması kullanılarak yapılan nörolojik değerlendirmeden sonra, lomber spinal kordlar, histopatolojik değerlendirme ve HSP70, interlökin-6 ve miyeloperoksidaz (MPO) için immünhistokimyasal boyama amacıyla çıkartıldı.BulGulAr: G1 grubundaki tüm sıçanların nörolojik durumu normaldi ve MDI skorları 0\'dı (p <0.05). G3\'ün MDI skoru, G2 grubuna göre anlamlı derecede düşük bulundu (2.8 vs 5.5; p <0.05 ). G1'de vaküoler konjesyon, diğer gruplara göre anlamlı derecede düşük bulundu (p = 0.0001). İnterlökin-6 reseptörü düzeyi, G3 grubunda kontrol grubuna göre istatistiksel olarak anlamlı düzeyde daha düşük bulundu (p = 0.013). HSP70 ve MPO boyanma derecesi açısından gruplar arasınd...

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Ipsilateral common iliac artery plus femoral artery clamping for inducing sciatic nerve ischemia/reperfusion injury in rats: a reliable and simple method

Cited by 7 publications

References 7 publications

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

Pharmacological evaluation of tacrolimus (FK-506) on ischemia reperfusion induced vasculatic neuropathic pain in rats

Effect of montelukast on spinal cord ischemia/reperfusion injury

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