iPSCs and Exosomes: Partners in Crime Fighting Cardiovascular Diseases

Germena, Giulia; Hinkel, Rabea

doi:10.3390/jpm11060529

Cited by 9 publications

(8 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…hiPSC-exosomes from pluripotent stem cells may inherit their advantages, which may enable more treatment Frontiers in Bioengineering and Biotechnology frontiersin.org approaches for diseases (Jung et al, 2017;Wang, 2021;Zhou et al, 2021). Exosomes derived from hiPSCs have the potential to treat skin and eye diseases, and especially cardiovascular diseases (Germena and Hinkel, 2021). With the wide applications of hiPSC-exosomes, the establishment of a safety evaluation system is urgent and important to boost the progress of research and clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells

Yin

Song

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

As a new cell-free therapy, exosomes have provided new ideas for the treatment of various diseases. Human induced pluripotent stem cells (hiPSCs) cannot be used in clinical trials because of tumorigenicity, but the exosomes derived from hiPSCs may combine the advantages of iPSC pluripotency and the nanoscale size of exosomes while avoiding tumorigenicity. Currently, the safety and biodistribution of hiPSC-exosomes in vivo are unclear. Here, we investigated the effects of hiPSC-exosomes on hemolysis, DNA damage, and cytotoxicity through cell experiments. We also explored the safety of vein injection of hiPSC-exosomes in rabbits and rats. Differences in organ distribution after nasal administration were compared in normal and Parkinson’s disease model mice. This study may provide support for clinical therapy and research of intravenous and nasal administration of hiPSC-exosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells

Yin

Song

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Previous investigations highlighted that exosomes could act as messengers for intercellular communications in cardiovascular diseases and cell-derived exosomes can serve as a potential alternative to stem cell therapy that mediates heart regeneration representing a rising niche for assessment and therapy of cardiovascular diseases [25][26][27]. In addition, previous studies have shown that exosomes derived from mesenchymal stem cells (MSCs) could inhibit myocardial inflammation, fibrosis, and apoptosis in AF mice [12].…”

Section: Discussionmentioning

confidence: 99%

Exosomes from Bone Marrow Mesenchymal Stem Cells with Overexpressed Nrf2 Inhibit Cardiac Fibrosis in Rats with Atrial Fibrillation

Fan

et al. 2022

Cardiovascular Therapeutics

View full text Add to dashboard Cite

Background. Even though nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling has been associated with the pathogenesis of multiple heart conditions, data on roles of Nrf2 within atrial fibrillation (AF) still remain scant. The present investigation had the aim of analyzing Nrf2-overexpressing role/s upon bone mesenchymal stem cell- (BMSC-) derived exosomes in rats with AF. Methods. Exosomes were collected from control or Nrf2 lentivirus-transduced BMSCs and then injected into rats with AF through the tail vein. AF duration was observed using electrocardiography. Immunohistochemical staining was then employed for assessing Nrf2, HO-1, α-SMA, collagen I, or TGF-β1 expression profiles within atrial myocardium tissues. Conversely, Masson staining was utilized to evaluate atrial fibrosis whereas apoptosis within myocardia was evaluated through TUNEL assays. In addition, TNF-α, IL-1β, IL-4, or IL-10 serum expression was assessed through ELISA. Results. Results of the current study showed significant downregulation of Nrf2/HO-1 within AF rat myocardia. It was found that injection of the control or Lv-Nrf2 exosomes significantly alleviated and lowered AF timespans together with reducing cardiomyocyte apoptosis. Moreover, injection of Lv-Nrf2 exosomes essentially lowered AF-driven atrial fibrosis and also inhibited inflammatory responses in the rats with AF. Conclusion. Delivery of BMSC-derived exosomes using overexpressed Nrf2 inhibited AF-induced arrhythmias, myocardial fibrosis, apoptosis, and inflammation via Nrf2/HO-1 pathway triggering.

show abstract

“…Generally, the function of exosomes depends on their cell of origin, and exosomes from young blood may rejuvenate aging tissues via various processes. A significant advantage of exosomes is that they do not form teratomas [ 10 , 11 ]. For example, intravenous administration of mesenchymal stem cell (MSC)-derived exosomes decreased myocardial and brain infarction areas [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

iPSC-derived exosomes promote angiogenesis in naturally aged mice

Li,

Zhang,

Wang

et al. 2023

Aging

View full text Add to dashboard Cite

Heterochronic parabiosis has shown that aging individuals can be rejuvenated by a youthful circulatory system; however, the underlying mechanisms remain unclear. Here, we evaluated the effect of exosomes isolated from mouse induced pluripotent stem cells (iPSCs) on angiogenesis in naturally aged mice. To achieve this, the angiogenic capacity of aortic ring, the total antioxidant capacity (TAOC), p53 and p16 expression levels of major organs, the proliferation of adherent bone marrow cells, and the function and content of serum exosomes in aged mice administered iPSC-derived exosomes were examined. Additionally, the effect of iPSC-derived exosomes on injured human umbilical vein endothelial cells (HUVECs) was assessed. The angiogenic capacity of aortic rings and clonality of bone marrow cells from young mice were significantly higher than those from aged mice; moreover, the organs of aged mice had a higher expression of aging genes and lower total TAOC. However, in vitro and in vivo experiments showed that the administration of iPSC-derived exosomes significantly improved these parameters in aged mice. The synergistic effect of both in vivo and in vitro treatments of aortic rings with iPSC-derived exosomes improved the angiogenic capacity of aortic rings from aged mice to levels similar to that of young mice. Compared with untreated aged mice, serum exosomal protein content and their promoted effect on endothelial cell proliferation and angiogenesis were significantly higher in untreated young mice and aged mice treated with iPSC-derived exosomes. Overall, these results showed that iPSC-derived exosomes may rejuvenate the body by anti-aging the vascular system.

show abstract

iPSCs and Exosomes: Partners in Crime Fighting Cardiovascular Diseases

Cited by 9 publications

References 85 publications

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells

Exosomes from Bone Marrow Mesenchymal Stem Cells with Overexpressed Nrf2 Inhibit Cardiac Fibrosis in Rats with Atrial Fibrillation

iPSC-derived exosomes promote angiogenesis in naturally aged mice

Contact Info

Product

Resources

About