iPSCs: A Comparison between Animals and Plants

Sang, Ya Lin; Cheng, Zhi Juan

doi:10.1016/j.tplants.2018.05.008

Cited by 27 publications

(19 citation statements)

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“…To substantiate the importance of autophagy in preserving phenotypic plasticity, we examined iPSC formation and organ regeneration—well‐known examples of somatic cell reprogramming—in autophagy‐deficient plants. Plant PSCs can be induced in vitro by adjusting the ratios of auxin and cytokinin to trigger dedifferentiation and proliferation of an unorganized mass of pluripotent cells (callus) (Sugimoto et al , ; Sang et al , ). When we placed WT, atg2‐2 , and atg5‐1 root explants in callus‐inducing medium (CIM) to induce PSC and callus formation (Valvekens et al , ), WT root explants dedifferentiated into PSC and formed visible calli, but atg2 and atg5 root explants did not (Fig A).…”

Section: Resultsmentioning

confidence: 99%

“…This allows somatic cells to redifferentiate into other cell types, organs, and even whole organisms in plants (Takahashi & Yamanaka, ; Papp & Plath, ; Ikeuchi et al , ; Li & Belmonte, ; Li et al , ). Similar to temporary reprogramming, reprogramming into other cell types is orchestrated by evolutionarily conserved processes and involves major changes in the transcriptome and epigenetic landscape (Roche et al , ; Sang et al , ; Iwafuchi‐Doi, ). Despite the wealth of knowledge on initial transcriptional and epigenetic changes driving somatic reprogramming events, how proteostasis mechanisms delete current cellular states to allow installment of new programs remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

et al. 2020

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Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell‐state switches in both plants and animals, but how cells rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during temporary reprogramming in plants triggered by phytohormones, immune, and danger signals. Quantitative proteomics following sequential reprogramming revealed that autophagy is required for timely decay of previous cellular states and for tweaking the proteome to acclimate to the new conditions. Signatures of previous cellular programs thus persist in autophagy‐deficient cells, affecting cellular decision‐making. Concordantly, autophagy‐deficient cells fail to acclimatize to dynamic climate changes. Similarly, they have defects in dedifferentiating into pluripotent stem cells, and redifferentiation during organogenesis. These observations indicate that autophagy mediates cell‐state switches that underlie somatic cell reprogramming in plants and possibly other organisms, and thereby promotes phenotypic plasticity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

et al. 2020

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“…Plant hormones play pivotal roles in regeneration (Birnbaum and Sánchez Alvarado, 2008;Sena and Birnbaum, 2010;Perianez-Rodriguez et al, 2014;Pulianmackal et al, 2014;Su and Zhang, 2014;Xu and Huang, 2014;Ikeuchi et al, 2016;Kareem et al, 2016;Sang et al, 2018). It is well known that cytokinin induces shoot regeneration whereas auxin promotes root regeneration (Skoog and Miller, 1957).…”

Section: Introductionmentioning

confidence: 99%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by woundinduced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

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“…This allows somatic cells to redifferentiate into other cell 14 types, organs and even whole organisms in plants (Ikeuchi et al, 2015;Li & Belmonte, 15 2017;Papp & Plath, 2013;Li et al, 2017;Takahashi & Yamanaka, 2006). Similar to 16 temporary reprogramming, reprogramming into other cell types is orchestrated by 17 evolutionarily conserved processes and involves major changes in the transcriptome 18 and epigenetic landscape (Iwafuchi-Doi, 2019;Roche et al, 2017;Sang et al, 2018). 19…”

mentioning

confidence: 99%

“…4). This indicates that the loss of cellular homeostasis 162 in atg mutants translates into higher heterogeneity, and this increased heterogeneity 163 might stem from their decreased ability to cope with daily variations in temperature, Plant pluripotent stem cells (PSCs) can be induced in vitro by adjusting the ratios of 173 auxin and cytokinin to trigger dedifferentiation and proliferation of an unorganized 174 mass of pluripotent cells (callus) (Sang et al, 2018;Sugimoto et al, 2010). When we 175 placed WT, atg2-2 and atg5-1 root explants in callus-inducing medium (CIM) to induce 176 PSC and callus formation ( (Valvekens et al, 1988)), WT root explants dedifferentiated 177 into PSC and formed visible calli, but atg2 and atg5 root explants did not (Fig.…”

mentioning

confidence: 99%

Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

Rodriguez

Chevalier

Olsen

et al. 2019

Preprint

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Somatic cells acclimate to changes in the environment by temporary reprogramming.Much has been learned about transcription factors that induce these cell-state switches in both plants and animals, but how cells rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during temporary reprogramming in plants triggered by phytohormones, immune and danger signals.Quantitative proteomics following sequential reprogramming revealed that autophagy is required for timely decay of previous cellular states and for tweaking the proteome to acclimate to the new conditions. Signatures of previous cellular programs thus persist in autophagy deficient cells, affecting cellular decision-making. Concordantly, autophagy deficient cells fail to acclimatize to dynamic climate changes. Similarly, they have defects in dedifferentiating into pluripotent stem cells, and redifferentiation during organogenesis. These observations indicate that autophagy mediates cell state switches that underlie somatic cell reprogramming in plants and possibly other organisms, and thereby promotes phenotypic plasticity.

show abstract

iPSCs: A Comparison between Animals and Plants

Cited by 27 publications

References 64 publications

Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells

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