iPSC bioprinting for musculoskeletal tissue

Romanazzo, Sara; Kilian, Kristopher A.; Roohani, Iman

doi:10.1016/b978-0-12-823809-7.00008-6

Cited by 1 publication

(1 citation statement)

References 88 publications

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“…Human induced pluripotent stem cells (hiPSCs) offer tremendous potential for 3D bioprinting tissues because of their capacity for generating limitless autologous differentiated cell types. [ 16 , 17 , 18 , 19 ] Furthermore, their ability to spontaneously form 3D hAs that can proliferate, undergo serial passaging, and differentiate into self‐assembled organoids entirely in suspension culture, offers a truly scalable process for engineering organs replete with organ‐like microarchitecture. [ 9 ] Automated stirred tank bioreactors are the industry standard for scalable bioprocessing, and have been developed for the large‐scale production of microbial and mammalian cells, and more recently, embryonic stem cells and hiPSCs.…”

Section: Introductionmentioning

confidence: 99%

Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors

Lee

et al. 2022

Adv Healthcare Materials

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Combining the sustainable culture of billions of human cells and the bioprinting of wholly cellular bioinks offers a pathway toward organ-scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and handling constraints. Here, the suspension culture of human induced pluripotent stem cell-derived aggregates (hAs) is optimized using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, and pluripotency marker expression are maintained over three serial passages in two distinct cell lines. Furthermore, it is demonstrated that the same optimized parameters can be scaled to an automated 1 L stirred tank bioreactor system. This 4-day culture results in a 16.6-to 20.4-fold expansion of cells, generating approximately 4 billion cells per vessel, while maintaining >94% expression of pluripotency markers. The pluripotent aggregates can be subsequently differentiated into derivatives of the three germ layers, including cardiac aggregates, and vascular, cortical and intestinal organoids. Finally, the aggregates are compacted into a wholly cellular bioink for rheological characterization and 3D bioprinting. The printed hAs are subsequently differentiated into neuronal and vascular tissue. This work demonstrates an optimized suspension culture-to-3D bioprinting pipeline that enables a sustainable approach to billion cell-scale organ engineering.

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Section: Introductionmentioning

confidence: 99%

Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors

Lee

et al. 2022

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

iPSC bioprinting for musculoskeletal tissue

Cited by 1 publication

References 88 publications

Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors

Large‐Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors

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