Ions Regulate Spore Attachment, Germination, and Fungal Growth

Shaw, Brian D.; Hoch, H. C.

doi:10.1007/978-3-540-70618-2_9

Cited by 6 publications

(5 citation statements)

References 140 publications

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“…Also, it may be that the highest sporulation obtained with MBSP could be due to a higher presence of free Ca +2 ions in the medium, which coincides with results described by Mosley et al (1989), Shaw and Hoch (2007), Koslova et al (2010), andHernández et al (2011).…”

Section: Silgadosupporting

confidence: 84%

In vitro selection of phosphate solubilizing strains of Trichoderma spp.

2016

Int. J. Biosci.

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Phosphorous (P) is considered to be the macronutrient with the lowest availability for plants in the majority of tropical soils, and is thus a limiting factor for vegetal growth. The application of Phosphoric Rock (PR) has been widely studied in order to satisfy its requirement in crops, but the quantity of liberated P is too low to satisfy demand. A viable alternative for managing this problem is the use of rhizospheric microorganisms that solubilize P (PSM) in chemically unavailable forms. In the search for microorganisms that solubilize phosphates, studies report that Trichoderma spp., in addition to their effects as pathogen biocontrollers, also exhibit phosphodissolvent capacities. The objective of this investigation was to evaluate in vitro strains of Trichoderma spp. isolated from rhizospheres of beans and corn with phosphate solubilizing abilities. Different strains of Trichoderma were isolated and selected from rhizospheric soil and rhizospheres. Three medium types were evaluated utilizing both solid and liquid media in order to determine their biometric characteristics, relative efficiency of solubilization of PR, bioacidulant capacity of the culture medium, and concentration of P in solution. In liquid media, these fungi were inoculated with and without PR, the quantity of soluble P was quantified, and the pH was taken. There were significant differences with respect to mycelial growth between solid media with and without the presence of PR, solubilization halos, and relative efficiency of solubilization in relation to incubation time. Liquid media with and without PR showed significant differences with respect to the quantity of soluble P. The pH of these media was inversely proportional to the quantity of soluble P, as the probable principle mechanism of dissolution of PR is the production of organic acids.

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Section: Silgadosupporting

confidence: 84%

In vitro selection of phosphate solubilizing strains of Trichoderma spp.

2016

Int. J. Biosci.

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“…pH has a strong influence on cell growth and metabolism (Schmid et al, ; Shaw and Hoch, ). It plays significant roles in the production of biomass, as well as cell metabolites such as exopolysaccharides (Shu and Lung, ; Wang and McNeil, ).…”

Section: Resultsmentioning

confidence: 99%

Effect of Media Components and Growth Conditions for Improved Linoleic Acid Production by Beauveria Species

Sanghi

Chirmade

et al. 2019

J Americ Oil Chem Soc

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Beauveria species are well-known insect pathogenic fungi, and Beauveria bassiana is used as a biopesticide against various pests in agriculture. However, the Beauveria species has not been reported as producers of microbial oils. In this study, Beauveria spp. MTCC 5184 was used to produce microbial oil with high linoleic acid (LA) content. Ten experiments were performed to evaluate the effects of several media parameters, such as carbon and nitrogen sources, pH, various concentrations of carbon and nitrogen, growth duration, and oleic acid (OLA) supplementation for maximum LA and dry biomass production by the fungus. Several of these parameters had a significant impact on the production of LA, as well as dry biomass. The glucose yeast extract (GYE) medium supplemented with 1.5% (w/v) peptone yielded maximum LA (0.32 AE 0.01 g L −1 ) and biomass (5.51 AE 0.26 g L −1 ). However, through the addition of 1.0% (w/v) OLA, the precursor of LA, LA production was enhanced 12-fold (1.24 AE 0.03 g L −1 ), and the biomass production increased by 5-fold (11.05 AE 0.46 g L −1 ) in comparison to those in the basal (GYE) medium. Using lactose as the sole carbon source produced the lowest LA (0.05 AE 0.00 g L −1 ) and biomass (1.04 AE 0.10 g L −1 ). The results of this study will be useful for the commercial exploitation of this fungus for the production of LA-rich microbial oil for use in the production of lubricants, greases, paints, cosmetics, etc.Keywords Linoleic acid Á Beauveria Á MTCC 5184 Á Oleic acid Á Gas chromatography Á Media optimization J Am Oil Chem Soc (2019) 96: 945-954.

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“…delica . Calcium is a non-specific signaling ion that also plays a central role in ion signaling pathways (for review see [ 54 ]) and in several important development processes of oomycetes such as encystment, adhesion and germination [ 55 – 57 ]. A variety of specialized structures such as those aiding ingresses of the fungal pathogen into its host, or adsorption of nutrients (appressoria, hyphodamia, haustoria, etc.)…”

Section: Discussionmentioning

confidence: 99%

Specialized attachment structure of the fish pathogenic oomycete Saprolegnia parasitica

et al. 2018

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The secondary cysts of the fish pathogen oomycete Saprolegnia parasitica possess bundles of long hooked hairs that are characteristic to this economically important pathogenic species. Few studies have been carried out on elucidating their specific role in the S. parasitica life cycle and the role they may have in the infection process. We show here their function by employing several strategies that focus on descriptive, developmental and predictive approaches. The strength of attachment of the secondary cysts of this pathogen was compared to other closely related species where bundles of long hooked hairs are absent. We found that the attachment of the S. parasitica cysts was around three times stronger than that of other species. The time sequence and influence of selected factors on morphology and the number of the bundles of long hooked hairs conducted by scanning electron microscopy study revealed that these are dynamic structures. They are deployed early after encystment, i.e., within 30 sec of zoospore encystment, and the length, but not the number, of the bundles steadily increased over the encystment period. We also observed that the number and length of the bundles was influenced by the type of substrate and encystment treatment applied, suggesting that these structures can adapt to different substrates (glass or fish scales) and can be modulated by different signals (i.e., protein media, 50 mM CaCl2 concentrations, carbon particles). Immunolocalization studies evidenced the presence of an adhesive extracellular matrix. The bioinformatic analyses of the S. parasitica secreted proteins showed that there is a high expression of genes encoding domains of putative proteins related to the attachment process and cell adhesion (fibronectin and thrombospondin) coinciding with the deployment stage of the bundles of long hooked hairs formation. This suggests that the bundles are structures that might contribute to the adhesion of the cysts to the host because they are composed of these adhesive proteins and/or by increasing the surface of attachment of this extracellular matrix.

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Ions Regulate Spore Attachment, Germination, and Fungal Growth

Cited by 6 publications

References 140 publications

In vitro selection of phosphate solubilizing strains of Trichoderma spp.

In vitro selection of phosphate solubilizing strains of Trichoderma spp.

Effect of Media Components and Growth Conditions for Improved Linoleic Acid Production by Beauveria Species

Specialized attachment structure of the fish pathogenic oomycete Saprolegnia parasitica

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