Ions, metabolites, and cells: Water as a reporter of surface conditions during bacterial growth

Jarisz, Tasha A.; Lane, Sarah; Gozdzialski, Lea; Hore, Dennis K.

doi:10.1063/1.5023748

Cited by 5 publications

(22 citation statements)

References 82 publications

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“…Moreover, when the same E. coli fractions were monitored with SFG spectroscopy, but with the cells removed, only samples from the late exponential and stationary phases showed an increase in interfacial water signal. 11 These fractions contain EPS with a higher uronic and nucleic acid content, and therefore have a greater capacity to sequester ions from the surface microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Time Evolution of Bacterial Extracellular Polymer Composition Studied with a Versatile Infrared Transmission Array Scanner

2019

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The chemical composition of extracellular polymeric substances (EPS) secreted by E. Coli K12 in solution was monitored as a function of time over various stages of bacterial growth. We have observed that the spectra become enhanced at 1132 cm −1 and 980 cm −1 , attributed to an increasing fraction of uronic and nucleic acids within the polymer matrix. Both of these acidic molecules have been shown to sequester cations from solution in order to neutralize their negative charge. We discuss the implication for this composition profile change in terms of previously observed restructuring of interfacial water molecules during the earliest stages of bacterial adhesion. To monitor each fraction of EPS under the same conditions, we illustrate the design and operation of a high throughput array scanner fitted to a Fourier-transform infrared spectrometer.

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Section: Discussionmentioning

confidence: 99%

Time Evolution of Bacterial Extracellular Polymer Composition Studied with a Versatile Infrared Transmission Array Scanner

2019

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“…The third case, the bacterium adheres to the surface and then begins secreting EPS that attract other bacterium to adhere to the surface. These models have been investigated using nonlinear vibrational spectroscopy, where recently it was discovered that the interfacial water above silica substrate appears to be change over the stages of bacterial growth, suggesting that changes in the microenvironment may play a role in bacterial adhesion [14,15].…”

Section: Adhesion Modelsmentioning

confidence: 99%

“…A motivation for this imaging study was to complement previous nonlinear vibrational spectroscopy work that suggested that the interfacial water between water-silica, the bacterial microenvironment, are sensitive to microenvironment changes during this 24 h growth period [14]. The same spectroscopy experiment was done with only bulk media, which the bacteria were grown in, over time, to determine whether factors contributed by changing bulk media, had an impact.…”

Section: Cell Adhesion At the Microenvironment Levelmentioning

confidence: 99%

“…16−22 Although specific markers from cells have not been observed, the inherent surface specificity of SFG enables cell adhesion to be indirectly monitored via changes in vibrational fingerprints of other molecules at the surface. We have recently used nonlinear vibrational spectroscopy to study the adhesion of E. coli K12 to a silica surface, 22 were proposed to account for this monotonic signal intensity increase. First, in the presence of cells, a unique microenvironment is established as a result of cell and EPS adhesion, resulting in a pH and ionic strength distinct from that of the bulk solution.…”

Section: ■ Introductionmentioning

confidence: 99%

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Monitoring Early Stages of Bacterial Adhesion at Silica Surfaces through Image Analysis

et al. 2020

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Understanding bacterial adhesion and biofilm formations on abiotic surfaces are important biological processes that affect the growth of bacteria, with its far-spreading impacts on in everyday life, either as a benefactor or as an inhibitor. To study these bacterial interactions, tools to probe these interfaces are also important to provide further means for discovery of the adhesion mechanisms. In this thesis, a customized imaging platform was developed, utilizing brightfield microscopy to study E. coli K12 on silica surfaces over the stages of bacteria growth. Results observed bacteria adhering onto silica surfaces in a preferential pattern to already existing bacteria-adhered colonies. This suggest that bacteria, once adhered to the surface, enhance attraction of other planktonic bacteria. The platform was designed to enable concurrent Raman measurements, with further optimization required in order to enhance the Raman signals from individual cells. Results from this study provide strong evidence to link changes in interfacial water structure with previous surface vibrational spectroscopy experiments, where the surface coverage of bacteria was found to reach a maximum earlier in the stages of growth compared to the surface water response, indicating that adhesion alone is not the primary contributing factor to modification of the surface microenvironment.

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“…As is well known, water plays a key role in mediating molecular interactions at silica surfaces [19]. Experimental and theoretical studies of bio-inorganic hybrids are normally performed in aqueous systems because water helps preserving the structure and biologic functionality of proteins [16,35,36,37,38]. Actually, the confinement of biosystems within nanosized water aggregates is by itself of high current interest, as it has recently emerged as a new technology to experimentally detect conformational changes of protein active sites [39].…”

Section: Introductionmentioning

confidence: 99%

Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels

Giussani

Tabacchi

Coluccia

et al. 2019

IJMS

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Incorporation of biological systems in water nanodroplets has recently emerged as a new frontier to investigate structural changes of biomolecules, with perspective applications in ultra-fast drug delivery. We report on the molecular dynamics of the digestive protein Pepsin subjected to a double confinement. The double confinement stemmed from embedding the protein inside a water nanodroplet, which in turn was caged in a nanochannel mimicking the mesoporous silica SBA-15. The nano-bio-droplet, whose size fits with the pore diameter, behaved differently depending on the protonation state of the pore surface silanols. Neutral channel sections allowed for the droplet to flow, while deprotonated sections acted as anchoring piers for the droplet. Inside the droplet, the protein, not directly bonded to the surface, showed a behavior similar to that reported for bulk water solutions, indicating that double confinement should not alter its catalytic activity. Our results suggest that nanobiodroplets, recently fabricated in volatile environments, can be encapsulated and stored in mesoporous silicas.

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Ions, metabolites, and cells: Water as a reporter of surface conditions during bacterial growth

Cited by 5 publications

References 82 publications

Time Evolution of Bacterial Extracellular Polymer Composition Studied with a Versatile Infrared Transmission Array Scanner

Time Evolution of Bacterial Extracellular Polymer Composition Studied with a Versatile Infrared Transmission Array Scanner

Monitoring Early Stages of Bacterial Adhesion at Silica Surfaces through Image Analysis

Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels

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