Ionophore A23187-induced leukotriene biosynthesis in equine granulocytes—neutrophils, but not eosinophils require exogenous arachidonic acid

Lindberg, Annelie; Tornhamre, Susanne; Mugnai, S.; Lindgren, Jan

doi:10.1016/s0005-2760(98)00005-8

Cited by 15 publications

(25 citation statements)

References 35 publications

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“…Equine neutrophils, unlike equine eosinophils or human neutrophils, require the addition of exogenous arachidonic acid for LT production. [19][20][21] Although arachidonic acid is likely to be present in inflammatory exudates, activated equine neutrophils collected from horses with heaves do not facilitate cholinergic bronchoconstriction, 8 suggesting that their production of cysteinyl LT may not be of a great magnitude. While there is evidence of increased LTD 4 production in airway secretion of humans with asthma, there are no quantitative studies in which the concentration of LTD 4 in lung secretions of horses with heaves have been measured.…”

Section: Discussionmentioning

confidence: 99%

Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction

Lavoie¹,

Léguillette²,

Pasloske³

et al. 2002

ajvr

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L-708,738 was bioavailable after oral administration and sustained concentrations in plasma during the dosing period that exceeded in vitro efficacy values. However, airway function did not improve, suggesting that either drug concentrations in the lungs were subtherapeutic or that cysteinyl LT may not be important mediators of airway inflammation in heaves. Results provide the first evidence of cysteinyl LT1 receptors in airways of horses.

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Section: Discussionmentioning

confidence: 99%

Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction

Lavoie¹,

Léguillette²,

Pasloske³

et al. 2002

ajvr

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“…4 were negligible (< 17 pmol/g of tissue, 5 pmol/ml tissue bath fluid) in both types of tissues in RAO-affected and control horses. Incubation of both tissue types with supplementation of exogenous arachidonic acid (8 µM) also failed to trigger substantial LT formation.…”

Section: Resultsmentioning

confidence: 83%

“…4 Briefly, tissue bath fluid and neutrophil suspension supernatant were centrifuged and evaporated to dryness. The samples were dissolved in mobile-phase high-performance liquid chromatography (HPLC) solution and recentrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…Once formed, LTC 4 is actively transported to the extracellular space, where it is metabolized to LTD 4 and LTE 4 by successive elimination of a γ-glutamyl residue and glycine, respectively. 3 Neutrophils can produce LTB 4 4 but lack the capacity to convert LTA 4 to LTC 4 , 5 whereas eosinophils produce LTC 4 . 4 The cysteinyl LT, LTC 4 and LTD 4 , are potent bronchoconstrictors and mucus secretagogues, and they provoke vascular leakage.…”

mentioning

confidence: 99%

“…3 Neutrophils can produce LTB 4 4 but lack the capacity to convert LTA 4 to LTC 4 , 5 whereas eosinophils produce LTC 4 . 4 The cysteinyl LT, LTC 4 and LTD 4 , are potent bronchoconstrictors and mucus secretagogues, and they provoke vascular leakage. 3 Furthermore, LTA 4 can be exported from neutrophils and further metabolized by surrounding cells to LTC 4 or LTB 4 via transcellular mechanisms.…”

mentioning

confidence: 99%

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Evaluation of leukotriene biosynthetic capacity in lung tissues from horses with recurrent airway obstruction

Lindberg¹,

Näsman-Glaser²,

Lindgren³

et al. 2002

ajvr

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Equine neutrophils and their role in ischemia reperfusion injury and lung inflammation

Anderson

Singh

2018

Cell Tissue Res

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Horses are susceptible to a multitude of inflammatory conditions that are characterized by a strong neutrophilic response. Here, we review basic equine neutrophil biology and explore the role of neutrophils in inflammatory conditions with emphasis on intestinal ischemia and reperfusion injury and lung inflammation. In addition, unique aspects of equine neutrophil biology have been highlighted. Neutrophils comprise the highest proportion of circulating white blood cells in equine blood. The concentration of circulating equine neutrophils is a primary indicator of systemic inflammation. Additionally, equine neutrophils exposed to various stimulants develop "toxic" changes characterized as cytoplasmic basophilia, presence of Döhle bodies, cytoplasmic vacuolation and toxic granulation. In contrast to human neutrophils, equine neutrophils fail to undergo chemotaxis in response to the peptide N-formyl-methionyl-leucyl-phenylalanine and are dependent on the addition of arachidonic acid due to reduced activity of phospholipase A to synthesize leukotrienes as part of the arachidonic acid pathway. Understanding the biologic function of neutrophils in horses is integral to developing methods to modulate inflammation associated with ischemia reperfusion injury and lung disease.

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Ionophore A23187-induced leukotriene biosynthesis in equine granulocytes—neutrophils, but not eosinophils require exogenous arachidonic acid

Cited by 15 publications

References 35 publications

Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction

Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction

Evaluation of leukotriene biosynthetic capacity in lung tissues from horses with recurrent airway obstruction

Equine neutrophils and their role in ischemia reperfusion injury and lung inflammation

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