Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple

Cao, Yang; Du, Peihua; Ji, Jiahao; He, Xiaolong; Zhang, Jiran; Shang, Yuwei; Liu, Huaite; Xu, Jin; Liang, Bowen

doi:10.3390/ijms23179855

Cited by 12 publications

(4 citation statements)

References 50 publications

(75 reference statements)

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“…Our study found that MT treatment increased P and Cd uptake by oat seedlings, promoted root elongation and secondary root production, and improved root vigor, and this change in root morphology facilitated plant adaptation to stress. The same conclusion was reached in a study by Cao et al [62] on apples. In addition, appropriate concentrations of MT can improve the salt tolerance of plants through improving the transport of Na, Ca, and Mg ions by their leaves and roots and reconfiguring the ionic balance.…”

Section: Effect On Mineral Element Contentsupporting

confidence: 83%

The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline–Alkali Stress

Wang,

Liang,

Xiang

et al. 2023

Agronomy

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Exogenous melatonin (MT) regulates plant growth and mitigates stress in response to stress. To analyze the machinery of exogenous melatonin, which improves salt and alkaline tolerance in oats, MT’s function was identified in the oat seed germination stage in our previous study. In this study, morphogenesis, photosynthetic physiology, hormone levels, and ion homeostasis were evaluated using the same MT treatment concentration. The results revealed that compared to the S45 treatment, the 100 μmol·L−1 MT treatment efficiently increased the seedling height and main root length of oat seedlings; promoted secondary root development; enhanced the root volume and root surface area; maintained a higher photosynthetic pigment content (carotenoids; chlorophyll a; chlorophyll b); raised the leaf photosynthetic rate (Pn), intercellular CO2 concentration (Ci), conductance to H2O (Gs), and transpiration rate (Tr); enhanced the light energy absorption and conversion of leaves; increased the leaf GA3, Tryptamine (TAM), and IAA contents; and decreased ABA levels. Hierarchical cluster analysis revealed that MT treatment also increased the contents of P, K, Ca, Mn, Cu, Mg, Fe, Zn, Mo, Cd, Al, Se, Ni, Co, and Ti; decreased the Na/K ratio; and maintained cellular ionic homeostasis in oat seedlings under saline–alkali stress, as compared with the untreated group. These findings showed that MT treatment enhanced the adaptation of oat to saline–alkali stress through regulating the physiological process of seedling growth. This suggests that MT plays a different role in improving saline–alkali tolerance in the germination and seedling stages of oat.

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Section: Effect On Mineral Element Contentsupporting

confidence: 83%

The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline–Alkali Stress

Wang,

Liang,

Xiang

et al. 2023

Agronomy

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“…Transcriptomic analysis revealed that genes engaged in different metabolite pathways, signal transduction, transcription factors, and kinase activity were upregulated in response to stress. Genes involved in energy metabolism encoding mitochondrial ATPase, cytochrome c, ferredoxin, sucrose, and starch metabolism were identified in DEGs between melatonin-treated and untreated plants [63,64]. Earlier findings of Zhao et al [65] confirmed that applying melatonin increases the expression level of genes involved in signal transduction and carbon metabolism of plants under stress, thereby improving the photosynthetic process.…”

Section: Discussionmentioning

confidence: 79%

Melatonin Enhances the Photosynthesis and Antioxidant Enzyme Activities of Mung Bean under Drought and High-Temperature Stress Conditions

Kuppusamy,

Alagarswamy,

Karuppusami

et al. 2023

Plants

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Mung bean, a legume, is sensitive to abiotic stresses at different growth stages, and its yield potential is affected by drought and high-temperature stress at the sensitive stage. Melatonin is a multifunctional hormone that plays a vital role in plant stress defense mechanisms. This study aimed to evaluate the efficiency of melatonin under individual and combined drought and high-temperature stress in mung bean. An experiment was laid out with five treatments, including an exogenous application of 100 µM melatonin as a seed treatment, foliar spray, and a combination of both seed treatment and foliar spray, as well as absolute control (ambient condition) and control (stress without melatonin treatment). Stresses were imposed during the mung bean’s reproductive stage (31–40 DAS) for ten days. Results revealed that drought and high-temperature stress significantly decreased chlorophyll index, Fv/Fm ratio, photosynthetic rate, stomatal conductance, and transpiration rate through increased reactive oxygen species (ROS) production. Foliar application of melatonin at 100 µM concentration enhanced the activity of antioxidant enzymes such as superoxide dismutase, catalase, and ascorbate peroxidase and the concentration of metabolites involved in osmoregulation and ion homeostasis; thereby, it improves physiological and yield-related traits in mung bean under individual and combined stress at the reproductive stage.

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“…Genomic data aided with ionomics, particularly in combinations with forward and reverse genetic approaches, can detect cellular changes during abiotic stress conditions [ 265 ]. Recent reports showed that ionomics studies revealed the mechanisms of ion uptake under abiotic stresses in plants [ 266 , 267 , 268 ]. Moreover, transport, compartmentalization and exclusion of ions during adverse environmental conations were also monitored by ionomics.…”

Section: Proteogenomics Lipidomics Ionomics and Interactomicsmentioning

confidence: 99%

“…Moreover, transport, compartmentalization and exclusion of ions during adverse environmental conations were also monitored by ionomics. Although ionomics is a new field and there are only a few reports of ionomics studies under abiotic stress available, the trends are growing for ionomics studies [ 266 , 267 , 268 ]. In a general sense, ionomics are related to the ion content of an organism (here in plants) that is required for its growth and developmental processes under different environments and growth stages [ 269 ].…”

Section: Proteogenomics Lipidomics Ionomics and Interactomicsmentioning

confidence: 99%

Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses

Roychowdhury

Das²,

Gupta

et al. 2023

Genes

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The present day’s ongoing global warming and climate change adversely affect plants through imposing environmental (abiotic) stresses and disease pressure. The major abiotic factors such as drought, heat, cold, salinity, etc., hamper a plant’s innate growth and development, resulting in reduced yield and quality, with the possibility of undesired traits. In the 21st century, the advent of high-throughput sequencing tools, state-of-the-art biotechnological techniques and bioinformatic analyzing pipelines led to the easy characterization of plant traits for abiotic stress response and tolerance mechanisms by applying the ‘omics’ toolbox. Panomics pipeline including genomics, transcriptomics, proteomics, metabolomics, epigenomics, proteogenomics, interactomics, ionomics, phenomics, etc., have become very handy nowadays. This is important to produce climate-smart future crops with a proper understanding of the molecular mechanisms of abiotic stress responses by the plant’s genes, transcripts, proteins, epigenome, cellular metabolic circuits and resultant phenotype. Instead of mono-omics, two or more (hence ‘multi-omics’) integrated-omics approaches can decipher the plant’s abiotic stress tolerance response very well. Multi-omics-characterized plants can be used as potent genetic resources to incorporate into the future breeding program. For the practical utility of crop improvement, multi-omics approaches for particular abiotic stress tolerance can be combined with genome-assisted breeding (GAB) by being pyramided with improved crop yield, food quality and associated agronomic traits and can open a new era of omics-assisted breeding. Thus, multi-omics pipelines together are able to decipher molecular processes, biomarkers, targets for genetic engineering, regulatory networks and precision agriculture solutions for a crop’s variable abiotic stress tolerance to ensure food security under changing environmental circumstances.

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Ionomic Combined with Transcriptomic and Metabolomic Analyses to Explore the Mechanism Underlying the Effect of Melatonin in Relieving Nutrient Stress in Apple

Cited by 12 publications

References 50 publications

The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline–Alkali Stress

The Physiological Mechanism of Melatonin Enhancing the Tolerance of Oat Seedlings under Saline–Alkali Stress

Melatonin Enhances the Photosynthesis and Antioxidant Enzyme Activities of Mung Bean under Drought and High-Temperature Stress Conditions

Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses

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