Ionized Ti Surfaces Increase Cell Adhesion Properties of Mesenchymal Stem Cells

Ferroni, Letizia; Gardin, Chiara; Bressan, Eriberto; Guirado, José Luis Calvo; Isola, Maurizio; Piattelli, Adriano; Zavan, Barbara

doi:10.1166/jbt.2015.1326

Cited by 7 publications

(8 citation statements)

References 25 publications

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“…Interestingly, as a result of this research, it has emerged that YB promotes cell differentiation through the activation of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway [35][36][37][38]. However, several differences have been pointed out between that study and our study.…”

Section: Discussioncontrasting

confidence: 57%

“…Pang and colleagues reported that YB powder is rich in calcium ions, and it is the main reason for its well-known hemostatic properties. In fact, calcium ions can stimulate the local production of hemostatic agents; moreover, the calcium ions interact with cytokines and growth factors, so stimulating the local bone formation [32][33][34][35]. Mesenchymal stem cells (MSCs) are located in several areas of the human body.…”

Section: Discussionmentioning

confidence: 99%

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The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

et al. 2019

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Bone regeneration involves several biological processes that consistently impact the quality of tissue healing. An important step consists of the local recruitment and differentiation of mesenchymal stem cells that migrate in the site to regenerate from bone marrow. Mesenchymal stem cells (MSCs) may be pushed towards osteogenic commitment by specific substances, often naturally present in plants. Yunnan Baiyao (YB) is a Chinese herbal medicine, mainly working through the synergic effect of terpenoid and steroidal saponins. YB is well known for its numerous biomedical effects, including the ability to favor improved bone tissue healing. In our in vitro study, we used adipose mesenchymal stem cells (ADSCs) as a study-model: We selected samples to harvest and isolate ADSCs and investigate their viability; moreover, we performed bone-related gene expression to evaluate the differentiation of MSCs. To confirm this behavior, we analyzed alkaline phosphate activity and calcium deposition, with ADSCs cultured in basal and osteogenic media, with YB at different concentrations in the medium, and at different time-points: 7, 14 and 21 days. Our results indicate that the synergic effect of terpenoid and steroidal saponins slightly favor the late ADSCs differentiation towards the osteoblasts phenotype. In osteogenic committed cells, the treatment with the lower dose of YB promoted the up-regulation of the alkaline phosphatase gene (ALPL) at day seven and 14 (p < 0.01); at day 21, the alkaline phosphatase (ALP) activity showed a slight increase, although in basal condition it maintains low rates. We assume that such molecular synergy can promote the osteogenic commitment of adipose mesenchymal stem cells, thus improving the timing and the quality of bone healing.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

et al. 2019

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“…Cell proliferation rate was investigated through the MTT-based assay according to the method previously described [81]. Briefly, 2 × 10 4 cells at p1, p3, and p5 were seeded onto 24-well plates in BM.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Potential of Autologous Adipose-Derived Stem Cells for the Treatment of Liver Disease

Gardin

Ferroni

Bellin

et al. 2018

IJMS

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Currently, the most effective therapy for liver diseases is liver transplantation, but its use is limited by organ donor shortage, economic reasons, and the requirement for lifelong immunosuppression. Mesenchymal stem cell (MSC) transplantation represents a promising alternative for treating liver pathologies in both human and veterinary medicine. Interestingly, these pathologies appear with a common clinical and pathological profile in the human and canine species; as a consequence, dogs may be a spontaneous model for clinical investigations in humans. The aim of this work was to characterize canine adipose-derived MSCs (cADSCs) and compare them to their human counterpart (hADSCs) in order to support the application of the canine model in cell-based therapy of liver diseases. Both cADSCs and hADSCs were successfully isolated from adipose tissue samples. The two cell populations shared a common fibroblast-like morphology, expression of stemness surface markers, and proliferation rate. When examining multilineage differentiation abilities, cADSCs showed lower adipogenic potential and higher osteogenic differentiation than human cells. Both cell populations retained high viability when kept in PBS at controlled temperature and up to 72 h, indicating the possibility of short-term storage and transportation. In addition, we evaluated the efficacy of autologous ADSCs transplantation in dogs with liver diseases. All animals exhibited significantly improved liver function, as evidenced by lower liver biomarkers levels measured after cells transplantation and evaluation of cytological specimens. These beneficial effects seem to be related to the immunomodulatory properties of stem cells. We therefore believe that such an approach could be a starting point for translating the results to the human clinical practice in future.

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“…The mutagenic potential of experimental and control Ti implants was evaluated with the Ames test by using the Salmonella Mutagenicity Complete Test Kit (Moltox, Molecular toxicology Inc., Boone, NC, USA), as described in Ferroni et al [30] Briefly, Ti implants were extracted for (24 ± 2) h at (37 ± 1)°C, using nutrient broth (blank) as the extraction vehicle. The same extraction conditions were set for Aluminium oxide ceramic rod (VITA In-Ceram Alumina CA-12, CE 0124, lot 15,320) (negative control), and ICR 191 Acridine (Moltox, 60-101) and Sodium Azide (Moltox, 60-103) (positive controls).…”

Section: Ames Testmentioning

confidence: 99%

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

Gardin

Ferroni

Piattelli

et al. 2016

Stem Cell Rev and Rep

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Surface topography of Titanium (Ti) dental implants strongly influences osseointegration. In the present work, we have analyzed the influence of two Ti implant surfaces characterized by similar microtopography but different nanotopography and chemistry on the osteoblastic phenotype of Dental Pulp Stem Cells (DPSCs). The effect on osteogenic differentiation, extracellular matrix (ECM) and cell adhesion molecules production have been evaluated by means of molecular biology analyses. The morphology of the cells grown onto these surfaces has been analyzed with SEM and immunofluorescence (IF), and the safety of the surfaces has been tested by using karyotype analysis, Ames test and hemocompatibility assay. Results showed that starting from 15 days of DPSCs culture, a substantial expression of osteoblast specific markers and a strong increase of cell adhesion molecules can be detected. In particular, when DPSCs are seeded on the Ti implants expression of microRNA (miRNA)-196a, which is involved in osteoblastic commitment of stem cells, and of Vascular Cell Adhesion Molecule 1 (VCAM1), a factor involved in angiogenesis, is strongly enhanced.

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Ionized Ti Surfaces Increase Cell Adhesion Properties of Mesenchymal Stem Cells

Cited by 7 publications

References 25 publications

The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

The Synergic Effect of Terpenoid and Steroidal Saponins Can Improve Bone Healing, by Promoting the Osteogenic Commitment of Adipose Mesenchymal Stem Cells: An In Vitro Study

Therapeutic Potential of Autologous Adipose-Derived Stem Cells for the Treatment of Liver Disease

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

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