Ionized jet deposition of silver nanostructured coatings: Assessment of chemico-physical and biological behavior for application in orthopedics

Graziani, Gabriela; Ghezzi, Daniele; Boi, Marco; Baldini, Nicola; Sassoni, Enrico; Cappelletti, Martina; Fedrizzi, Giorgio; Maglio, Melania; Salamanna, Francesca; Tschon, Matilde; Martini, Lucia; Zaffagnini, Stefano; Fini, Milena; Sartori, Maria

doi:10.1016/j.bioadv.2024.213815

Cited by 2 publications

(3 citation statements)

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“…To overcome these issues, the design of implant material has been implemented with bioactive functionalization, with antimicrobial properties or actively enhancing tissue regeneration and implant integration. These materials also serve as carriers for a controlled delivery of bioactive molecules or drugs [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

Maglio,

Fini,

Sartori

et al. 2024

IJMS

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In the field of biomaterials for prosthetic reconstructive surgery, there is the lack of advanced innovative methods to investigate the potentialities of smart biomaterials before in vivo tests. Despite the complex osteointegration process being difficult to recreate in vitro, this study proposes an advanced in vitro tissue culture model of osteointegration using human bone. Cubic samples of trabecular bone were harvested, as waste material, from hip arthroplasty; inner cylindrical defects were created and assigned to the following groups: (1) empty defects (CTRneg); (2) defects implanted with a cytotoxic copper pin (CTRpos); (3) defects implanted with standard titanium pins (Ti). Tissues were dynamically cultured in mini rotating bioreactors and assessed weekly for viability and sterility. After 8 weeks, immunoenzymatic, microtomographic, histological, and histomorphometric analyses were performed. The model was able to simulate the effects of implantation of the materials, showing a drop in viability in CTR+, while Ti appears to have a trophic effect on bone. MicroCT and a histological analysis supported the results, with signs of matrix and bone deposition at the Ti implant site. Data suggest the reliability of the tested model in recreating the osteointegration process in vitro with the aim of reducing and refining in vivo preclinical models.

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Section: Introductionmentioning

confidence: 99%

“…In the modern era, high technological innovations in materials and their manufacturing contribute to the development of intelligent and multifunctional biomaterials, able to interact with the host bone and promote tissue regeneration by inductive and cell-and molecular-mediated effects [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

Maglio,

Fini,

Sartori

et al. 2024

IJMS

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“…The use of coatings in the prevention of bacterial infections is becoming increasingly important as the demand for safe and hygienic medical equipment and surfaces increases ( Pietsch et al, 2020 ; Boi et al, 2022 ). Most of inorganic coatings are functionalized with metallic materials such as titanium dioxide, silver, copper, and zinc oxide, which have natural antimicrobial properties ( Graziani et al, 2021 ; 2024 ; La Torre et al, 2021 ; Ghezzi et al, 2023a ; 2023b ). Metals have been used for centuries in various medical applications and are now gaining attention thanks to their pleiotropic activities as antimicrobial agents ( Harrison et al, 2007 ; Turner, 2017 ; Pormohammad et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

New strontium-based coatings show activity against pathogenic bacteria in spine infection

Ghezzi,

Graziani,

Cappelletti

et al. 2024

Front. Bioeng. Biotechnol.

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Infections of implants and prostheses represent relevant complications associated with the implantation of biomedical devices in spine surgery. Indeed, due to the length of the surgical procedures and the need to implant invasive devices, infections have high incidence, interfere with osseointegration, and are becoming increasingly difficult to threat with common therapies due to the acquisition of antibiotic resistance genes by pathogenic bacteria. The application of metal-substituted tricalcium phosphate coatings onto the biomedical devices is a promising strategy to simultaneously prevent bacterial infections and promote osseointegration/osseoinduction. Strontium-substituted tricalcium phosphate (Sr-TCP) is known to be an encouraging formulation with osseoinductive properties, but its antimicrobial potential is still unexplored. To this end, novel Sr-TCP coatings were manufactured by Ionized Jet Deposition technology and characterized for their physiochemical and morphological properties, cytotoxicity, and bioactivity against Escherichia coli ATCC 8739 and Staphylococcus aureus ATCC 6538P human pathogenic strains. The coatings are nanostructured, as they are composed by aggregates with diameters from 90 nm up to 1 μm, and their morphology depends significantly on the deposition time. The Sr-TCP coatings did not exhibit any cytotoxic effects on human cell lines and provided an inhibitory effect on the planktonic growth of E. coli and S. aureus strains after 8 h of incubation. Furthermore, bacterial adhesion (after 4 h of exposure) and biofilm formation (after 24 h of cell growth) were significantly reduced when the strains were cultured on Sr-TCP compared to tricalcium phosphate only coatings. On Sr-TCP coatings, E. coli and S. aureus cells lost their organization in a biofilm-like structure and showed morphological alterations due to the toxic effect of the metal. These results demonstrate the stability and anti-adhesion/antibiofilm properties of IJD-manufactured Sr-TCP coatings, which represent potential candidates for future applications to prevent prostheses infections and to promote osteointegration/osteoinduction.

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Ionized jet deposition of silver nanostructured coatings: Assessment of chemico-physical and biological behavior for application in orthopedics

Cited by 2 publications

References 63 publications

An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

New strontium-based coatings show activity against pathogenic bacteria in spine infection

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