Ionized calcium and 1,25-dihydroxyvitamin D concentration in serum of patients with sarcoidosis

Hamada, Kunio; Nagai, Sonoko; Tsutsumi, Takanobu; Izumi, Tatsuro

doi:10.1183/09031936.98.11051015

Cited by 32 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serum levels of 1,25(OH) 2 D 3 are inversely correlated with Ca 2þ levels [Holick, 1996;Hamada et al, 1998]. Our findings reveal that levels of 1,25(OH) 2 D 3 causing increased DNA, Lowry protein, and PG encompass the range found by Bikle et al [1985] in hypocalcemic serum.…”

Section: Discussionmentioning

confidence: 53%

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes

Genge

Ishikawa

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

Time- and dosage-dependent effects of 1,25(OH)(2)D(3) and 24,25(OH)(2)D(3) on primary cultures of pre- and post-confluent avian growth plate (GP) chondrocytes were examined. Cultures were grown in either a serum-containing culture medium designed to closely mimic normal GP extracellular fluid (DATP5) or a commercially available serum-free media (HL-1) frequently used for studying skeletal cells. Hoechst DNA, Lowry protein, proteoglycan (PG), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activity and calcium and phosphate mineral deposition in the extracellular matrix were measured. In preconfluent cultures grown in DATP5, physiological levels of 24,25(OH)(2)D(3) (0.10-10 nM) increased DNA, protein, and LDH activity significantly more than did 1,25(OH)(2)D(3) (0.01-1.0 nM). However, in HL-1, the reverse was true. Determining ratios of LDH and PG to DNA, protein, and each other, revealed that 1,25(OH)(2)D(3) specifically increased PG, whereas 24,25(OH)(2)D(3) increased LDH. Post-confluent cells were generally less responsive, especially to 24,25(OH)(2)D(3). The positive anabolic effects of 24,25(OH)(2)D(3) required serum-containing GP-fluid-like culture medium. In contrast, effects of 1,25(OH)(2)D(3) were most apparent in serum-free medium, but were still significant in serum-containing media. Administered to preconfluent cells in DATP5, 1,25(OH)(2)D(3) caused rapid, powerful, dosage-dependent inhibition of Ca(2+) and Pi deposition. The lowest level tested (0.01 nM) caused >70% inhibition during the initial stages of mineral deposition; higher levels of 1,25(OH)(2)D(3) caused progressively more profound and persistent reductions. In contrast, 24,25(OH)(2)D(3) increased mineral deposition 20-50%; it required >1 week, but the effects were specific, persistent, and largely dosage-independent. From a physiological perspective, these effects can be explained as follows: 1,25(OH)(2)D(3) levels rise in hypocalcemia; it stimulates gut absorption and releases Ca(2+) from bone to correct this deficiency. We now show that 1,25(OH)(2)D(3) also conserves Ca(2+) by inhibiting mineralization. The slow anabolic effects of 24,25(OH)(2)D(3)are consistent with its production under eucalcemic conditions which enable bone formation. These findings, which implicate serum-binding proteins and accumulation of PG in modulating accessibility of the metabolites to GP chondrocytes, also help explain some discrepancies previously reported in the literature.

show abstract

Section: Discussionmentioning

confidence: 53%

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes

Genge

Ishikawa

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…The level of calcitriol is positively correlated with the disease activity (21,22). Elevated levels of vitamin 1, 25(OH)2D downregulate dendritic cells leading to attenuation of the immune response.…”

Section: The Role Of Vitamin 125(oh)2d In Sarcoidosismentioning

confidence: 98%

The role of vitamin D in multisystem sarcoidosis / Uloga vitamina D u sarkoidozi

Mihailović-Vučinić¹,

Ignjatović²,

Dudvarski-Ilić³

et al. 2012

Journal of Medical Biochemistry

View full text Add to dashboard Cite

Summary: Recently published data indicate that vitamin D abnormalities are common in sarcoidosis patients. The purpose of this study was to compare serum vitamin 25(OH)D levels among sarcoidosis patients with different clinical courses of the disease. The study also included the first observations on cognitive functions (i.e. depression and fatigue syndrome) in relation to vitamin D deficiency in sarcoidosis patients. At the Biochemical Laboratory of the Clinical Center of Serbia, Belgrade, vitamin D25(OH)D was measured using the Elecsys ® Vitamin D total test. A total of 226 patients with biopsy-positive sarcoidosis were analyzed. The average median value of serum vitamin D was 9.47 mg/L, suggesting severe deficiency. Statistically significant correlation was found in patients with chronic disease and low levels of serum vitamin 25(OH)D (Chi-Square=6.044; df=2; p=0.014). The patient group with vitamin D serum levels higher than 20 mg/L showed higher levels of the mean forced vi tal capacity (FVC) by 380 mL, and forced expiratory volume in one second (FEV1) by 220 mL, when compared to the patient group with lower serum vitamin D. A statistically significant role was established for serum vitamin 25(OH)D levels as the predictor of fatigue (R 2 =0.878; p=0.038 (b=0.216)) and depression in patients with sarcoidosis (R 2 =0.80; p=0.000 (b=0.391)). The insufficiency of 25(OH)D seems to be an important factor in predicting the course of chronic disease, significant lung function impairments and cognitive failures such as fatigue and depression. The fact that the majo rity of the analyzed sarcoidosis patients had totally deficient serum 25(OH)D levels made this finding even more notable. Keywords: vitamin D, deficiency, sarcoidosisKratak sadr`aj: Nedavno objavljena istra`ivanja kod obole lih od sarkoidoze govore o ~estim abnormalnim vrednostima vitamina D. Cilj ove studije bio je da se uporedi nivo vita mina 25(OH)D kod obolelih od sarkoidoze sa razli~itim klini~kim tokom bolesti. Tako|e, ova studija predstavlja prva za pa `anja o vezi izme|u kognitivnih funkcija (odnosno ose}a ja depresije i zamora) i deficita vitamina D kod obolelih od sarko idoze. U Biohemijskoj laboratoriji Klini~kog centra Srbije vitamin D -25(OH)D meren je kori{}enjem testa Elec sys ® Vitamin D. Analizirano je 226 bolesnika sa sarkoidozom potvr|enom biopsijom. Prose~na srednja vrednost vitamina D u serumu bila je 9,47 mg/L, {to ukazuje na ozbiljan nedostatak. Statisti~ki zna~ajna korelacija na |e na je kod pa cije nata sa hroni~nom formom bolesti i niskim nivoom vitamina 25(OH)D u serumu (Xi-kvad rat=6,044; df=2; p=0,014). Grupa pacijenata sa nivoom vitamina D u serumu ve}im od 20 mg/L pokazuje ve}i nivo srednjeg for siranog vitalnog kapaciteta (FVC) za 380 mL i forsiranog ekspi ratornog volumena u prvoj sekundi (FEV1) za 220 mL u od nosu na grupu pacijenata sa ni`im nivoom D vitamina. Utvr|eno je da nivo vitamina 25(OH)D u serumu ima stati sti~ki zna~ajnu ulogu kao prediktor zamora i depresije kod obolelih od sarkoidoze. Insuficijencija 25(OH)D ...

show abstract

“…Hypercalcemia occurs in 10%of the patients with sarcoidosis whereashypercalcinuria occurs more commonly, being present in up to 60% (9). In our previous report, 44% of patients with sarcoidosis had hypercalcemia or hypercalcinuria ( 10). Several cases associated with abnormal renal function in the absence of hypercalcemia, hypercalcinuria, nephrocalcinosis and urolithiasis as in case 2 have been reported.…”

Section: Discussionmentioning

confidence: 95%

Sarcoidosis with Granulomatous Interstitial Nephritis. Report of Three Cases.

et al. 2001

View full text Add to dashboard Cite

Three cases of sarcoidosis with granulomatous interstitial nephritis are reported. Patients were all male and over 50 years of age. They simultaneously had evidence of multiorgan involvement of sarcoidosis including lung and skin and/or eye. In addition, distinct features were found in each case: a granulomatous infiltration mimicking unilateral renal tumor (case 1); renal insufficiency solely due to granulomatous interstitial nephritis (case 2); and renal insufficiency with calcemic nephropathy and granulomatous interstitial nephritis (case 3). Prednisolone therapy resulted in disappearance of the pseudotumor in case 1 and partial improvementof renal function in cases 2 and 3. In cases 2 and 3, however, plasma creatinine did not return to normal values and a second renal biopsy in case 2 demonstrated residual interstitial fibrosis and few granulomas, suggesting that steroid therapy did not achieve complete reversal of changes. (Internal Medicine 40: 241-245, 2001)

show abstract

Ionized calcium and 1,25-dihydroxyvitamin D concentration in serum of patients with sarcoidosis

Cited by 32 publications

References 28 publications

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes

The role of vitamin D in multisystem sarcoidosis / Uloga vitamina D u sarkoidozi

Sarcoidosis with Granulomatous Interstitial Nephritis. Report of Three Cases.

Contact Info

Product

Resources

About

Ionized calcium and 1,25-dihydroxyvitamin D concentration in serum of patients with sarcoidosis

Cited by 32 publications

References 28 publications

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D3 on mineralizing growth plate chondrocytes

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D3 on mineralizing growth plate chondrocytes

The role of vitamin D in multisystem sarcoidosis / Uloga vitamina D u sarkoidozi

Sarcoidosis with Granulomatous Interstitial Nephritis. Report of Three Cases.

Contact Info

Product

Resources

About

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes

Effects of 24R,25‐ and 1α,25‐dihydroxyvitamin D₃ on mineralizing growth plate chondrocytes