IONIC REQUIREMENTS OF
            <i>TREPONEMA PALLIDUM</i>
            I

Doak, G. O.; Freedman, Leon D.; Clark, John W.

doi:10.1128/jb.77.3.322-327.1959

Cited by 5 publications

(2 citation statements)

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“…Defined media, designated the all-potassium and all-sodium media, were prepared as described in the previous paper (Doak et al, 1959). A suspension of actively motile treponemes was obtained by extracting sliced syphilitic rabbit testes with inactivated normal rabbit serum.…”

Section: Methodsmentioning

confidence: 99%

“…Five-ml portions of the treponeme suspension were added to a series of tubes containing various amounts of the salts to be tested; the tubes were then incubated in a Brewer anaerobe jar in an atmosphere of nitrogen and carbon dioxide (95 and 5 per cent) at 35 C. The jars were opened at intervals and the percentage of motile treponemes determined in each tube. Five counts were made on each tube, and the average value was calculated by the method given in the footnote to table 1 of the previous paper (Doak et al, 1959).…”

Section: Methodsmentioning

confidence: 99%

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IONIC REQUIREMENTS OF TREPONEMA PALLIDUM II

1959

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Doak et al. (1959) demonstrated the beneficial effects of low concentrations of sodium and potassium ions and the detrimental effect of higher concentrations of sodium ion on the survival in vitro of Treponema pallidum. Since this microorganism has not been cultivated in vitro, it cannot be said that either ion is essential for growth. However, most bacteria require potassium for growth (Lester, 1958), and it is not surprising that this ion prolongs survival time. The beneficial effect of sodium ion was unexpected, although MacLeod demonstrated that certain marine bacteria require sodium (MacLeod et al., 1954; MacLeod and Onofrey, 1956). The red halophile, Pseudomonas salinaria, also requires this ion (Brown and Gibbons, 1955). Many investigators have demonstrated that rubidium ion can replace potassium ion as a growth factor for bacteria and as an activator for isolated enzyme systems (Lester, 1958; Brown and Gibbons, 1955; Kachmar and Boyer, 1953; Black, 1931). An exception lhas recently been found in certain marine bacteria in which rubidium does not replace potassium (MacLeod and Onofrey, 1957). Cesium, in isolated enzyme systems, can partially replace potassium (Pressman and Lardy, 1952), but ammonium ion is known to sometimes replace potassium (Kachmar and Boyer, 1953; Stadtman, 1952; Von Korff, 1953) and at other times to be inhibitory (Black, 1931). Again, in certain isolated enzyme systems inhibited by sodium ion, this same inhibition can be achieved with lithium ion (Kachmar and Boyer, 1953; Black, 1931). The present work

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%